An atmospheric pressure ionization source using a high voltage target compared to electrospray ionization for the LC/MS analysis of pharmaceutical compounds

Lubin, Arnaud; Vries, Ronald de; Cabooter, Deirdre; Augustijns, Patrick; Cuyckens, Filip

doi:10.1016/j.jpba.2017.05.003

Cited by 16 publications

(16 citation statements)

References 25 publications

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“…Furthermore, several studies have demonstrated the differences on the ionization of specific classes of compounds and differences effects of the matrix, observed between different sources [6–11]. It is therefore of high interest for LC-MS/MS pesticide residue analysis, to evaluate the performances of newly introduced ionization sources in order to highlight their benefits and limitations in comparison with the source that is most commonly applied, i.e., electrospray ionization (ESI) [12].…”

Section: Introductionmentioning

confidence: 99%

Comparison of electrospray and UniSpray, a novel atmospheric pressure ionization interface, for LC-MS/MS analysis of 81 pesticide residues in food and water matrices

et al. 2019

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In mass spectrometry, the type and design of ionization source play a key role on the performance of a given instrument. Therefore, it is of paramount importance to evaluate newly developed sources for their suitability to analyze food contaminants like pesticide residues. Here, we carried out a head-to-head comparison of key extraction and analytical performance parameters of an electrospray ionization (ESI) source with a new atmospheric pressure ionization source, UniSpray (US). The two interfaces were evaluated in three matrices of different properties (coffee, apple, and water) to determine if multiresidue analysis of 81 pesticides by QuEChERS extraction and LC-MS/MS analysis could be improved. Depending on the matrix and irrespective of the chemical class, US provided a tremendous gain in signal intensity (22- to 32-fold in peak area, 6- to 7-fold in peak height), a threefold to fourfold increase in signal-to-noise ratio, a mild gain in the range of compounds that can be quantified, and up to twofold improvement of recovery. UniSpray offered comparable linearity and precision of the analyses with ESI, and did not affect the ion ratio. A gain in sensitivity of many compounds was observed with US, but in general, the two ionization interfaces did not show significant difference in LOD and LOQ. UniSpray suffered less signal suppression; the matrix effect was in average 3 to 4 times more pronounced, but showed better values than ESI. With no effect on recovery efficiency, US improved the overall process efficiency 3 to 4 times more than ESI. Graphical abstract Electronic supplementary material The online version of this article (10.1007/s00216-019-01886-z) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Comparison of electrospray and UniSpray, a novel atmospheric pressure ionization interface, for LC-MS/MS analysis of 81 pesticide residues in food and water matrices

et al. 2019

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“…Acetonitrile of analytical grade, dimethyl sulfoxide (DMSO) (for HPLC, >99.7%) and formic acid were purchased from Merck (Darmstadt, Germany). All MOTS-c peptides including human (h), mouse (m), human (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13) , human (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12) and rat (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14) analogues (purity ≥95%) were obtained from Phoenix Pharmaceuticals, Inc. (Burlingame, CA, USA). As internal standard (ISTD), six-fold 13 Tris-(2-carboxyethyl)phosphine hydrochloride (≥98%, TCEP) was from Carl Roth GmbH + Co. KG (Karlsruhe, Germany).…”

Section: Chemicals and Materialsmentioning

confidence: 99%

“…The reconstituted analytes were analyzed by means of high‐performance liquid chromatography coupled via UniSpray™ (US) ionization to triple quadrupole tandem mass spectrometry. US represents a new atmospheric pressure ionization (API) technique which is attributed to improved intensity gain (sensitivity) in comparison with ESI sources and reduced matrix effects for plasma samples . Supplementing the analytical capacity, animal variants as well as truncated derivatives, which are commercially available, were characterized by high‐resolution mass spectrometry (HRMS).…”

Section: Introductionmentioning

confidence: 99%

“…US represents a new atmospheric pressure ionization (API) technique which is attributed to improved intensity gain (sensitivity) in comparison with ESI sources and reduced matrix effects for plasma samples. 9,10 Supplementing the analytical capacity, animal variants as well as truncated derivatives, which are commercially available, were characterized by high-resolution mass spectrometry (HRMS). In vitro metabolic studies and stability tests were conducted to identify potential metabolic degradation and oxidation products.…”

Section: Introductionmentioning

confidence: 99%

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Development of a mass spectrometry based detection method for the mitochondrion‐derived peptide MOTS‐c in plasma samples for doping control purposes

Knoop

Thomas

Thevis

2019

Rapid Comm Mass Spectrometry

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Rationale The mitochondrial open reading frame of 12S rRNA type‐c (MOTS‐c) peptide was recently discovered and described to control metabolic homeostasis through AMPK activation along with AICAR accumulation. Consequently, it appears advisable to monitor the potential use of synthetic MOTS‐c in sports, and a detection method suitable for sports drug testing purposes is necessary. Methods For the detection of MOTS‐c in doping control plasma samples, a test method employing liquid chromatography and mass spectrometry (LC/MS) was developed. Following optimization, the assay was comprehensively validated and additional parameters such as the (long‐term) stability and in vitro metabolism of the peptide were evaluated. In order to determine endogenous MOTS‐c reference limits, the results generated by LC/MS‐based detection were compared with those obtained with a commercially available enzyme‐linked immunosorbent assay (ELISA). Results The LC/MS‐based test method was fully validated for quantitative results interpretation according to the World Anti‐Doping Agency's International Standard for Laboratories (WADA's ISL). It was found to be specific and sensitive, enabling a lower limit of detection (LLOD) for hMOTS‐c in plasma at 100 pg/mL. Following optimization, animal MOTS‐c analogues and four metabolites as well as two oxidation products were implemented. However, endogenous levels of a reference population of 20 healthy subjects studied by ELISA experiments (45.9–218.5 ng/mL) could not be confirmed by LC/MS. Conclusions A mass spectrometric detection assay for MOTS‐c in human plasma samples was developed and successfully validated according to WADA's ISL, providing an additional tool for future doping control purposes. Besides MOTS‐c, the assay also includes four in vitro derived metabolites and two oxidation products, which might further improve the traceability of the drug. The analytical approach was compared with a commercially available ELISA, and considerable differences in measured MOTS‐c levels were observed.

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“…One of the main current techniques for small molecular analysis is liquid chromatography-mass spectrometry (LC-MS). It has some limitations such as matrix effects and the unavoidable loss of pathological information or active ingredients during sample preparation [4,5]. Progress in research can be hindered by analytical limitations, which often demands rapid and direct analysis as well as high-throughput sample screening.…”

Section: Introductionmentioning

confidence: 99%

Magnetic silica nanoparticles for use in matrix-assisted laser desorption ionization mass spectrometry of labile biomolecules such as oligosaccharides, amino acids, peptides and nucleosides

Yang

Wishnok

et al. 2019

Microchim Acta

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Magnetic silica nanoparticles for use in matrix-assisted laser desorption ionization mass spectrometry of labile biomolecules such as oligosaccharides, amino acids, peptides and nucleosides The MIT Faculty has made this article openly available. Please share how this access benefits you. Your story matters. Citation Yang, H. et al. "Magnetic silica nanoparticles for use in matrixassisted laser desorption ionization mass spectrometry of labile biomolecules such as oligosaccharides, amino acids, peptides and nucleosides.

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An atmospheric pressure ionization source using a high voltage target compared to electrospray ionization for the LC/MS analysis of pharmaceutical compounds

Cited by 16 publications

References 25 publications

Comparison of electrospray and UniSpray, a novel atmospheric pressure ionization interface, for LC-MS/MS analysis of 81 pesticide residues in food and water matrices

Comparison of electrospray and UniSpray, a novel atmospheric pressure ionization interface, for LC-MS/MS analysis of 81 pesticide residues in food and water matrices

Development of a mass spectrometry based detection method for the mitochondrion‐derived peptide MOTS‐c in plasma samples for doping control purposes

Magnetic silica nanoparticles for use in matrix-assisted laser desorption ionization mass spectrometry of labile biomolecules such as oligosaccharides, amino acids, peptides and nucleosides

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