An ATP-Binding Cassette Transporter Gene from Cucumis sativus L., CsABC19, Is Involved in Propamocarb Stress in Arabidopsis thaliana

Meng, Jingjing; Qin, Zhiwei; Zhou, Xiuyan; Xin, Ming

doi:10.1007/s11105-016-0976-0

Cited by 10 publications

(16 citation statements)

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“…Not only may these genes function in stress responses, but in stress tolerance as well [ 43 , 44 ]. Gene-expression patterns are often associated with gene function [ 24 ]. In a previous study, the transcriptome changes in cucumber fruit of cultivar ‘D0351’ in response to PM treatment were analyzed by our research group.…”

Section: Discussionmentioning

confidence: 99%

“…Young plants at 34 days after transplanting at the 3-leaf stage were used for expression analysis of CsDIR16 in response to PM treatment. 8 mM PM solution was sprayed to similar sizes plants for 1 min until the surface of the leaves and fruits began to drip [ 21 , 23 , 24 ]. The leaves, stems, roots, and fruit peels (ca.…”

Section: Methodsmentioning

confidence: 99%

“…Transcriptomic analysis revealed that CsABC19 and CsWRKY30 are 2 positive regulators of plant tolerance to PM stress. Overexpression of CsABC19 and CsWRKY30 in Arabidopsis enhanced tolerance to PM stresse and decreased PM residues [ 24 , 25 ]. CsDIR16 is another key gene involved in responses to PM stress, as revealed by transcriptomic analysis.…”

Section: Introductionmentioning

confidence: 99%

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Expression and functional analysis of the Propamocarb-related gene CsDIR16 in cucumbers

et al. 2018

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BackgroundCucumber downy mildew is among the most important diseases that can disrupt cucumber production. Propamocarb, also known as propyl-[3-(dimethylamino)propyl]carbamate (PM), is a systemic carbamate fungicide pesticide that is widely applied in agricultural production because of its high efficiency of pathogens control, especially cucumber downy mildew. However, residual PM can remain in cucumbers after the disease has been controlled. To explore the molecular mechanisms of PM retention, cucumber cultivars ‘D9320’ (with the highest residual PM content) and ‘D0351’ (lowest residual PM content) were studied. High-throughput tag-sequencing (Tag-Seq) results showed that the CsDIR16 gene was related to PM residue, which was verified using transgenic technology.ResultsWe investigated the activity of a dirigent cucumber protein encoded by the CsDIR16 in gene response to stress induced by PM treatment. Gene-expression levels of CsDIR16 were up-regulated in the fruits, leaves, and stems of ‘D0351’ plants in response to PM treatment. However, in cultivar ‘D9320’, CsDIR16 levels were down-regulated in the leaves and stems after PM treatment, with no statistically significant differences observed in the fruits. Induction by jasmonic acid, abscisic acid, polyethylene glycol 4000, NaCl, and Corynespora cassiicola Wei (Cor) resulted in CsDIR16 up-regulation in ‘D0351’ and ‘D9320’. Expression after salicylic acid treatment was up-regulated in ‘D0351’, but was down-regulated in ‘D9320’. CsDIR16 overexpression lowered PM residues, and these were more rapidly reduced in CsDIR16(+) transgenic ‘D9320’ plants than in wild-type ‘D9320’ and CsDIR16(−) transgenic plants.ConclusionsAnalyses of the CsDIR16-expression patterns in the cucumber cultivars with the highest and lowest levels of PM residue, and transgenic validation indicated that CsDIR16 plays a positive role in reducing PM residues. The findings of this study help understand the regulatory mechanisms occurring in response to PM stress in cucumbers and in establishing the genetic basis for developing low-pesticide residue cucumber cultivars.Electronic supplementary materialThe online version of this article (10.1186/s12870-018-1236-2) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Expression and functional analysis of the Propamocarb-related gene CsDIR16 in cucumbers

et al. 2018

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“…For example, several studies have concentrated on the regulatory effects of cytochrome P450, glutathione, and the ABC transporter protein on pesticide metabolism in plants (Shiota et al, 1996;Brazier-Hicks et al, 2008;Meng et al, 2016). The GC technique has been widely used to analyze pesticide residues of relatively small molecular weight, high volatility, and heat-stability (González-Rodríguez et al, 2008;Swarnam and Velmurugan, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…Genes encoding resistant proteins participating in the propamocarb stress response were identified from cucumber variety D0351, which has low levels of propamocarb residue (Wu et al, 2013a,b). Overexpression of the CsABC19 gene enhanced the growth ability and resistance of Arabidopsis thaliana subjected to propamocarb stress (Meng et al, 2016). Studies of pesticide residues in vegetables have been limited to the detection of microscopic residues, and few studies have investigated the correlation between heredity of cucumber variety and pesticide residues.…”

Section: Introductionmentioning

confidence: 99%

Genetic analysis of molecular markers for propamocarb residue in Cucumis sativus using quantitative trait locus mapping

Xin

Wang

et al. 2016

Genet. Mol. Res.

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ABSTRACT. The use of pesticides to protect plants against harmful organisms, such as pathogenic microorganisms, is one of the most effective ways to improve agricultural production. However, the continuous use of pesticides might present a risk to human health, animals, and the environment. In this study, two cucumber (Cucumis sativus) varieties containing different levels of pesticide residues, D9320 and D0351, were selected to establish an F 2 population. A genetic model and genetic linkage map were constructed. The results showed that the heredity of pesticide residues was dominated by an additive effect and was significantly influenced by non-additive factors in cucumber. QCp1 was detected as a quantitative trait locus (QTL) that might be involved in regulating the levels of pesticide residue in 2 M. Xin et al. Genetics and Molecular Research 15 (4): gmr15048776 cucumber. Moreover, the cucumber genetic map was compared with the LG6 map, and the results indicated that this QTL was closely related to the level of pesticide residue in cucumber.

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Visualization and metabolome for the migration and distribution behavior of pesticides residue in after-ripening of banana

Wang

Tian

et al. 2023

Journal of Hazardous Materials

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An ATP-Binding Cassette Transporter Gene from Cucumis sativus L., CsABC19, Is Involved in Propamocarb Stress in Arabidopsis thaliana

Cited by 10 publications

References 50 publications

Expression and functional analysis of the Propamocarb-related gene CsDIR16 in cucumbers

Expression and functional analysis of the Propamocarb-related gene CsDIR16 in cucumbers

Genetic analysis of molecular markers for propamocarb residue in Cucumis sativus using quantitative trait locus mapping

Visualization and metabolome for the migration and distribution behavior of pesticides residue in after-ripening of banana

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