An attempt to analyze various thyroid stimulators by the receptor assay using hTSH radioimmunoassay.

Ochi, Yukio; Hachiya, Takashi; Miyazaki, Tadayoshi; Kajita, Yoshihiro; Yoshimura, Manabu

doi:10.1507/endocrj1954.26.1

Cited by 2 publications

(2 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1979] or thyroid carcinoma [Lahneche, 1977;Savoie and Massin, 1977;Beierwaltes, 1978b;Attie e\ al., 1979]. Furthermore, the incorporation or secretion of radioiodine by the thyroid has been used as an end point in some thyrotropin (TSH) bioassays [Inada et al, 1979;Ochi et al, 1979;Harada et al, 1979]. While it has long been recognized that TSH stimulates radioactive iodine incorporation and release by the thyroid [McKenzie.…”

Section: Introductionmentioning

confidence: 99%

Dose-Related Effect of Growth Hormone on Thyroidal Radioiodine Uptake

Ching¹

1981

Horm Res

View full text Add to dashboard Cite

Even though growth hormone was reported to play a role in mammalian calorigenesis, few studies have examined its effect on thyroid function. Consequently, the modulatory effect of bovine growth hormone (BGH) on thyroid incorporation of ¹³¹I was studied in hypophysectomized (H) rats. Not surprisingly, H recipients fed a low iodine diet consistently displayed greater thyroidal uptakes of radioactive iodine than recipients given regular diets. Furthermore, thyroid radioactive counts increased predictably in direct proportion to the quantity of bovine thyrotropin administered. Larger doses of BGH suppressed the thyroidal uptake of ¹³¹I whereas smaller doses either had no effect or enhanced thyroidal activity. Thus, these results affirm that radiolabelled and non-labelled iodine compete in thyroid metabolic processes and furthermore demonstrate that high doses of BGH inhibit thyroid activity.

show abstract

Section: Introductionmentioning

confidence: 99%

Dose-Related Effect of Growth Hormone on Thyroidal Radioiodine Uptake

Ching¹

1981

Horm Res

View full text Add to dashboard Cite

show abstract

“…However, the McKenzie bioassay for TSH is still being used in cases where information concerning biological acti¬ vity is deemed necessary (Rousset et al 1977;Harada et al 1979;Ochi et al 1979). Past studies have explored the mechanisms of this bioassay (Florsheim et al 1970) and examined the effect of TSH in plasma and pituitary extracts (Rerup & Melander 1965;Hershman 1970).…”

mentioning

confidence: 99%

Regarding the validity of the endpoint response of the mouse (McKenzie) bioassay for thyrotrophin (TSH)

Ching¹

1981

Acta Endocrinologica

View full text Add to dashboard Cite

The release of radiolabelled thyroid hormone into the circulation in low iodine fed mice has been used extensively as a bioassay for thyroid stimulating hormone (TSH). However, the specificity of several bioassays of pituitary hormones have been subject to question. Consequently, the validity of the assay endpoint for TSH in the mouse was re-evaluated with respect to the effect of luteinizing hormone (LH) whose chemical composition closely resembles that of TSH. Mice, prepared for bioassay of TSH received injections of purified LH or \g=a\ or \ g = b \ subunits of LH. Identical doses of LH and LH subunits were quantified by LH and TSH radioimmunoassays and the results compared with those obtained by the bioassay. Microgram quantities of LH and subunits of LH elicited appreciable responses in the TSH bioassay but produced only negligible effects in the TSH radioimmunoassay. The response of the TSH bioassay of LH and \g=a\ or \ g = b \ subunits of LH was 40\p=n-\56%that obtained with LH radioimmunoassay. However, the pituitary concentrations obtained by TSH bioassay when compared with those obtained by radioimmunoassays for TSH, LH, or growth hormone (GH) paralleled closely the TSH radioimmunoassay data, although in terms of quantitative estimates, there was a 15-fold discrepancy between the TSH assays. Estimations of pituitary concentrations of LH lead to the conclusion that, at the doses normally employed, most crude rat pituitary extracts do not contain sufficient quantities of LH to alter significantly bioassayable (McKenzie) estimates of TSH.

show abstract

An attempt to analyze various thyroid stimulators by the receptor assay using hTSH radioimmunoassay.

Cited by 2 publications

References 7 publications

Dose-Related Effect of Growth Hormone on Thyroidal Radioiodine Uptake

Dose-Related Effect of Growth Hormone on Thyroidal Radioiodine Uptake

Regarding the validity of the endpoint response of the mouse (McKenzie) bioassay for thyrotrophin (TSH)

Contact Info

Product

Resources

About