An attempt to determine discriminate use of hydrogen isotopes by bacteria

Abstract

The hydrogenase system of Hydrogenomonas facilis could be induced by either hydrogen or deuterium and the rate of deuterium uptake by the induced cells was the same as that of hydrogen. An attempt has been made to detect discriminate use of hydrogen and deuterium by bacteria. No discrimination could be detected during hydrogenation of methylene blue by Escherichia coli, under the experimental conditions.