An Attempt to Establish the Lipase Gene Sequence of <i>Alcaligenes </i>Sp. JG3 Using Internal Primer

Abstract

Alcaligenes sp. JG3 is a local strain bacterium from Indonesia, isolated from cultivated corn field of Central Java. This bacterium is able to produce lipase with fairly high activity. In order to do lipase gene sequence characterization, two sets of primer pair were used in this study (primer Fjg3 5’- ATGACCGAGCTGACTGTAG-3’, Rjg3 5’-TCAGGAGGGGTAAATCCAC-3’ and internal primer Fi 5’-TGACCCATGACCAGGCGGAA-3’ and Ri 5’-TTCGCCTGGTCATGGGTCA-3’). The complete lipase JG3 gene sequence consists of 1081 bp from start codon ATG to the stop codon of TGA. Lipase JG3 had high similarity to another lipase from genus Alcaligenaceae which was up to 90%. However, the 3D protein visualization analysis indicated that this lipase JG3 also has the characteristic of ABC transporter protein.