An Attempt to Increase the Heat Resistance of <i>Microbacterium lacticum</i>

Anagnostopoulos, Gerasimos; Seaman, A.; Woodbine, M.

doi:10.1111/j.1365-2672.1966.tb03469.x

Cited by 5 publications

(4 citation statements)

References 6 publications

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“…Several early workers described delayed development of colonies on agar following heat treatment and attributed this to extended lag (Nelson & Baker 1954;Lawton & Nelson 1955;Heather & Vanderzant 1957). The length of delay in colony formation was proportional to the severity of the heat stress (Anagnostopoulos et al 1966).…”

Section: Discussionmentioning

confidence: 99%

The effect of sublethal injury by heating, freezing, drying and gamma‐radiation on the duration of the lag phase of Salmonella typhimurium

Mackey

Derrick

1982

Journal of Applied Bacteriology

115

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The duration of the lag phase of Salmonella typhimurium surviving heat, freezing, drying and gamma‐radiation was used to indicate the time needed to repair sublethal injury. Following equivalent lethal treatments, heat and freeze‐injured cells needed longer to repair than those injured by drying or gamma‐radiation. Measurement of repair on membrane filters showed that in a heat‐injured population having a lag time of 9 h, some individual cells needed up to 14 h to recover maximum tolerance to 3% NaCl.

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Section: Discussionmentioning

confidence: 99%

The effect of sublethal injury by heating, freezing, drying and gamma‐radiation on the duration of the lag phase of Salmonella typhimurium

Mackey

Derrick

1982

Journal of Applied Bacteriology

115

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“…In addition, to provide further detail of strain 2C T , a measurement of the concentration and type of lactic acid produced after incubation at 30 °C in TSB for 48 h in the presence of 2.5 g l −1 glucose was conducted using d-lactic acid/l-lactic acid kit (r-Biopharm). Heat resistance in milk has been observed within the genus Microbacterium [37][38][39]. An experimental trial on strain 2C T was performed using partially skimmed UHT milk as substrate, testing three temperature/time conditions: 75 °C/15 s, 85 °C/15 s, and 95 °C/15 s, starting from a cell concentration of 10 5 c.f.u.…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

Microbacterium paulum sp. nov., isolated from microfiltered milk

Bellassi

Fontana

Callegari

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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A novel Gram-stain-positive, strictly aerobic, short rod-shaped bacterium, designated 2CT, was isolated from freshly packaged microfiltered milk. This strain was able to grow within the NaCl concentration range of 0–5 % (w/v), temperature range of 8–37 °C (optimally at 30 °C) and at pH 6.0–10.0. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 2CT was closely related to species of the genus Microbacterium , with the highest sequence similarity (99.2 %) to Microbacterium lacticum DSM 20427T as well as Microbacterium flavum DSM 18909T (=YM18-098T). The phylogenetic tree based on 16S rRNA genes showed that strain 2CT clustered with M. flavum DSM 18909T. However, the phylogenetic tree based on concatenated 16S rRNA and four housekeeping genes showed that strain 2CT clustered with M. lacticum DSM 20427T. Furthermore, the phylogenomic tree showed that strain 2CT clustered with M. lacticum DSM 20427T and M. flavum DSM 18909T. The major respiratory quinones were MK-10, MK-11 and MK-12. The predominant cellular fatty acids were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0. The polar lipid composition of strain 2CT consisted of diphosphatidylglycerol, phosphatidylglycerol, three unidentified glycolipids and two unidentified lipids. The cell-wall peptidoglycan type was a variant of B1α {Gly} [l-Lys] d-Glu-l-Lys, with the amino acids lysine, glycine, alanine and glutamic acid. The whole-cell sugars consisted of galactose, glucose, ribose and minor amounts of rhamnose. In addition, strain 2CT showed a glycolyl-type cell wall. The genomic DNA G+C content was 69.8mol%, while the average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values with the closely related Microbacterium species were below the recognized thresholds of 95–96 % ANI and 70 % DDH for species definition. Based on the phenotypic and genotypic data, strain 2CT (=LMG 32277T=CECT 30329T) is considered to represent a new species, for which the name Microbacterium paulum sp. nov. is proposed.

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“…The length of the delay in colony formation on agar plates, i.e. the time of lag, is dependent on the severity of the [heat] stress given (Kaufman et al 1959;Jackson and Woodbine 1963;Anagnostopoulos et al 1966;Payne 1978). The greatest variation in lags was observed with populations which had undergone the greatest level of stress.…”

Section: Introductionmentioning

confidence: 99%

“…the time of lag, is dependent on the severity of the [heat] stress given (Kaufman et al . 1959; Jackson & Woodbine 1963; Anagnostopoulos et al . 1966; Payne 1978).…”

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confidence: 99%

An Investigation into the differences between the Bioscreen and the traditional plate count disinfectant test methods

Lambert

Ouderaa

1999

Journal of Applied Microbiology

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Investigations of biocide efficacy by automated methods involving optical density measurements, e.g. using the recently published 'Bioscreen' method, are complicated by the fact that a poor correlation often exists between the log reductions obtained using the automated method vs those obtained by the traditional plate count methods. It was hypothesized that the differences observed between the two methods were due to the level of cell injury, which was masked by the optical density methods but which was recognized by the plate counts. Comparisons of log reductions following a disinfection test always showed the Bioscreen method to be overestimating the log reductions with respect to the plate counts. A correlation between colony size on the plates and the 'Bioscreen' results for a fixed disinfectant concentration and contact time was found using Global Imaging software. The results obtained also suggested that the observed colony area was dependent on the amount of injury incurred by a microbe during the disinfection process. A mathematical model of injury was developed which predicted the observed differences between the Bioscreen and the traditional plate method. The model further suggested a possible reason for biocidal lags.

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An Attempt to Increase the Heat Resistance of Microbacterium lacticum

Cited by 5 publications

References 6 publications

The effect of sublethal injury by heating, freezing, drying and gamma‐radiation on the duration of the lag phase of Salmonella typhimurium

The effect of sublethal injury by heating, freezing, drying and gamma‐radiation on the duration of the lag phase of Salmonella typhimurium

Microbacterium paulum sp. nov., isolated from microfiltered milk

An Investigation into the differences between the Bioscreen and the traditional plate count disinfectant test methods

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