An attenuated Salmonella enterica serovar Typhimurium strain lacking the ZnuABC transporter induces protection in a mouse intestinal model of Salmonella infection

Pesciaroli, Michele; Aloisio, Fabio; Ammendola, Serena; Pistoia, Claudia; Petrucci, Paola; Tarantino, Michela; Francia, Massimiliano; Battistoni, Andrea; Pasquali, Paolo

doi:10.1016/j.vaccine.2010.12.111

Cited by 31 publications

(26 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Unvaccinated piglets showed a progressive increase of S. Typhimurium shedding throughout the observational period, while piglets vaccinated either intramuscularly or orally showed a reduced and self-limiting shedding of wt S. Typhimurium, although different degrees between groups were noted. These findings, obtained in an experimental setting resembling farm conditions, corroborate and extend that reported earlier both in mice [1,17,21] and in pigs [20]. On that account, it is interesting to point out that the infection, acquired by direct contact with piglets shedding wt S. Typhimurium, showed marked differences to the pattern observed when we infected animals by an oral challenge [19,20].…”

Section: Discussionsupporting

confidence: 90%

“…The disease is usually characterized by self-limiting gastroenteritis in humans, but may occasionally cause fever and systemic infection [1,2]. Salmonellosis along with being a public health concern, is a economic problem as it also affects livestock breeding [3,4].…”

Section: Introductionmentioning

confidence: 99%

“…This Salmonella strain is attenuated due to its inability to efficiently recruit zinc in the infected host and it is protective in both mouse and pig models of salmonellosis [1,[17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Parenteral administration of attenuated Salmonella Typhimurium ΔznuABC is protective against salmonellosis in piglets

Ruggeri

Pesciaroli

Gaetarelli

et al. 2014

Vaccine

Self Cite

View full text Add to dashboard Cite

a b s t r a c tA major cause of salmonellosis in humans is the contamination of pork products. Infection in pigs can be controlled using bio-security programs, but they are not sufficient in countries where a high level of infection is recorded. In this context, the use of vaccines can represent a valid supplementary method of control. Recently, we have demonstrated that an attenuated strain of Salmonella enterica serovar Typhimurium (Salmonella Typhimurium znuABC) is protective against systemic and enteric salmonellosis in mouse and pig infection models, candidating this strain as an oral attenuated vaccine. In this study, we compared the efficacy of this attenuated Salmonella Typhimurium strain when administered orally or parenterally. Furthermore, in order to reproduce a pseudo-natural infection model, vaccinated pigs were allocated in the same pen with animals shedding virulent Salmonella Typhimurium. Animals were monitored weekly after vaccination and contact with infected piglets. Diarrhea and ataxia were recorded and Salmonella shedding was tested individually through bacterial culture. After four weeks of cohousing, piglets were euthanized, after which lymph nodes reactivity and gross lesions of the gut sections were scored at necropsy. Organs were submitted to microbiological and histological analyses.The data reported herein show that parenterally vaccinated animals do not shed the attenuated strain, and at the same time the absence of symptoms and decrease in virulent strain shedding in feces from day 6 after challenge demonstrated protection against infection induced by virulent Salmonella Typhimurium. In conclusion, our findings suggest that this is an alternative route of Salmonella Typhimurium znuABC administration, without ignoring the advantages associated with oral vaccination.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Parenteral administration of attenuated Salmonella Typhimurium ΔznuABC is protective against salmonellosis in piglets

Ruggeri

Pesciaroli

Gaetarelli

et al. 2014

Vaccine

Self Cite

View full text Add to dashboard Cite

show abstract

“…Metal homeostasis in bacteria is a delicate balance, especially since metals like zinc and iron have been found to be essential for the pathogenicity of these organisms (Cerasi et al 2014;Gradassi et al 2013;Pesciaroli et al 2011). The fact that protozoan grazing might be a strong force on keeping or gaining resistances against copper or zinc in nature together with the data suggesting that the concentrations necessary to maintain resistance plasmids within a population are well below the minimal inhibitory concentration (MIC) of the nonplasmid containing susceptible stain (Gullberg et al 2014) might explain the prevalence of resistance mechanisms.…”

Section: Copper and Zinc Resistance In Gram-positive Bacteriamentioning

confidence: 99%

Survival in amoeba—a major selection pressure on the presence of bacterial copper and zinc resistance determinants? Identification of a “copper pathogenicity island”

Hao

Lüthje

Qin

et al. 2015

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

The presence of metal resistance determinants in bacteria usually is attributed to geological or anthropogenic metal contamination in different environments or associated with the use of antimicrobial metals in human healthcare or in agriculture. While this is certainly true, we hypothesize that protozoan predation and macrophage killing are also responsible for selection of copper/zinc resistance genes in bacteria. In this review, we outline evidence supporting this hypothesis, as well as highlight the correlation between metal resistance and pathogenicity in bacteria. In addition, we introduce and characterize the Bcopper pathogenicity island^identified in Escherichia coli and Salmonella strains isolated from copper-and zinc-fed Danish pigs.

show abstract

“…Oral inoculation with this strain, protected mice against a challenge with 2 £ 10 8 CFU of pathogenic S, Typhimurium, reducing colonization in the spleen and cecum and the number of virulent bacteria in the gut, as well as decreasing intestinal inflammation. 71,72 S. Typhimurium Dhfq STMDhfq. This attenuated strain was obtained by the deletion of the hfq gene, encoding a small RNA chaperone involved in post-transcriptional regulation of gene expression in enterobacteria.…”

Section: Vaccines In Preclinical Stages Of Developmentmentioning

confidence: 99%

Vaccines for viral and bacterial pathogens causing acute gastroenteritis: Part II: Vaccines forShigella,Salmonella,enterotoxigenicE. coli(ETEC) enterohemorragicE. coli(EHEC) andCampylobacter jejuni

O’Ryan

Vidal

Canto

et al. 2015

Human Vaccines & Immunotherapeutics

View full text Add to dashboard Cite

An attenuated Salmonella enterica serovar Typhimurium strain lacking the ZnuABC transporter induces protection in a mouse intestinal model of Salmonella infection

Cited by 31 publications

References 31 publications

Parenteral administration of attenuated Salmonella Typhimurium ΔznuABC is protective against salmonellosis in piglets

Parenteral administration of attenuated Salmonella Typhimurium ΔznuABC is protective against salmonellosis in piglets

Survival in amoeba—a major selection pressure on the presence of bacterial copper and zinc resistance determinants? Identification of a “copper pathogenicity island”

Vaccines for viral and bacterial pathogens causing acute gastroenteritis: Part II: Vaccines forShigella,Salmonella,enterotoxigenicE. coli(ETEC) enterohemorragicE. coli(EHEC) andCampylobacter jejuni

Contact Info

Product

Resources

About