An Atypical psbA Gene Encodes a Sentinel D1 Protein to Form a Physiologically Relevant Inactive Photosystem II Complex in Cyanobacteria

Wegener, Kimberly M.; Nagarajan, Aparna; Pakrasi, Himadri B.

doi:10.1074/jbc.m114.604124

Cited by 34 publications

(46 citation statements)

References 57 publications

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“…Murray, () reported rD1 is a non‐functional version due to the absence of key amino acid residues essential for binding the oxygen evolving CaMn 4 O 5 clusters. Wegener and colleagues () also suggested that in Cyanothece this protein is incorporated into PSII complexes to eliminate their ability to evolve oxygen. Taking into account a large‐scale disassembly of PSII and the overall decrease in intracellular Chl during this phase, the abundance of PSII complexes containing rD1 was negligible as compared to the level of functional PSII complexes during the light phase.…”

Section: Discussionmentioning

confidence: 99%

Diel regulation of photosynthetic activity in the oceanic unicellular diazotrophic cyanobacterium Crocosphaera watsonii WH8501

Masuda

Bernát

Bečková

et al. 2018

Environmental Microbiology

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The oceanic unicellular diazotrophic cyanobacterium Crocosphaera watsonii WH8501 exhibits large diel changes in abundance of both Photosystem II (PSII) and Photosystem I (PSI). To understand the mechanisms underlying these dynamics, we assessed photosynthetic parameters, photosystem abundance and composition, and chlorophyll-protein biosynthesis over a diel cycle. Our data show that the decline in PSII activity and abundance observed during the dark period was related to a light-induced modification of PSII, which, in combination with the suppressed synthesis of membrane proteins, resulted in monomerization and gradual disassembly of a large portion of PSII core complexes. In the remaining population of assembled PSII monomeric complexes, we detected the non-functional version of the D1 protein, rD1, which was absent in PSII during the light phase. During the dark period, we also observed a significant decoupling of phycobilisomes from PSII and a decline in the chlorophyll a quota, which matched the complete loss of functional PSIIs and a substantial decrease in PSI abundance. However, the remaining PSI complexes maintained their photochemical activity. Thus, during the nocturnal period of nitrogen fixation C. watsonii operates a suite of regulatory mechanisms for efficient utilization/recycling of cellular resources and protection of the nitrogenase enzyme.

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Section: Discussionmentioning

confidence: 99%

Diel regulation of photosynthetic activity in the oceanic unicellular diazotrophic cyanobacterium Crocosphaera watsonii WH8501

Masuda

Bernát

Bečková

et al. 2018

Environmental Microbiology

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“…Several types of D1 can be distinguished phylogenetically (Cardona, Murray, & Rutherford, 2015) and their evolution is schematized in Figure 1c. The early evolving forms, referred to as atypical D1 forms (G0, G1, G2 in Figure 1), are characterized by the absence of some, but not all, of the ligands to the Mn 4 CaO 5 cluster and have been recently found to be involved in the synthesis of chlorophyll f, which supports oxygenic photosynthesis using low energy far-red light (Ho, Shen, Canniffe, Zhao, & Bryant, 2016;Nurnberg et al, 2018); or the inactivation of PSII when anaerobic processes are being carried out (Murray, 2012;Wegener, Nagarajan, & Pakrasi, 2015). The late evolving forms, referred to as the standard D1 forms, are characterized by a complete set of ligands to the Mn 4 CaO 5 cluster and are the main D1 used for water oxidation.…”

Section: Introductionmentioning

confidence: 99%

Early Archean origin of Photosystem II

et al. 2018

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Photosystem II is a photochemical reaction center that catalyzes the light‐driven oxidation of water to molecular oxygen. Water oxidation is the distinctive photochemical reaction that permitted the evolution of oxygenic photosynthesis and the eventual rise of eukaryotes. At what point during the history of life an ancestral photosystem evolved the capacity to oxidize water still remains unknown. Here, we study the evolution of the core reaction center proteins of Photosystem II using sequence and structural comparisons in combination with Bayesian relaxed molecular clocks. Our results indicate that a homodimeric photosystem with sufficient oxidizing power to split water had already appeared in the early Archean about a billion years before the most recent common ancestor of all described Cyanobacteria capable of oxygenic photosynthesis, and well before the diversification of some of the known groups of anoxygenic photosynthetic bacteria. Based on a structural and functional rationale, we hypothesize that this early Archean photosystem was capable of water oxidation to oxygen and had already evolved protection mechanisms against the formation of reactive oxygen species. This would place primordial forms of oxygenic photosynthesis at a very early stage in the evolutionary history of life.

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“…1), are characterized by the absence of some, but not all of the ligands to the Mn 4 CaO 5 cluster and have been recently found to be involved in the synthesis of chlorophyll f, which supports oxygenic photosynthesis using low energy far-red light (M. Y. Ho et al, 2016); or the inactivation of PSII when anaerobic process are being carried out such as nitrogen fixation (Murray, 2012;Wegener et al, 2015). The late evolving forms, referred to as the standard D1 forms, are characterized by a complete set of ligands to the Mn 4 CaO 5 cluster and are the main D1 used for water oxidation.…”

Section: Introductionmentioning

confidence: 99%

Early Archean origin of Photosystem II

Cardona

Sánchez‐Baracaldo

Rutherford

et al. 2017

Preprint

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Photosystem II is a photochemical reaction center that catalyzes the light-driven oxidation of water to molecular oxygen. Water oxidation is the distinctive photochemical reaction that permitted the evolution of oxygenic photosynthesis and the eventual rise of Eukaryotes. At what point during the history of life an ancestral photosystem evolved the capacity to oxidize water still remains unknown.Here we study the evolution of the core reaction center proteins of Photosystem II using sequence and structural comparisons in combination with Bayesian relaxed molecular clocks. Our results indicate that a homodimeric photosystem with sufficient oxidizing power to split water had already appeared in the early Archean about a billion years before the most recent common ancestor of all described Cyanobacteria capable of oxygenic photosynthesis, and well before the diversification of some of the known groups of anoxygenic photosynthetic bacteria. Based on a structural and functional rationale we hypothesize that this early Archean photosystem was capable of water oxidation and had already evolved some level of protection against the formation of reactive oxygen species, which would place primordial forms of oxygenic photosynthesis at a very early stage in the evolutionary history of life.

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An Atypical psbA Gene Encodes a Sentinel D1 Protein to Form a Physiologically Relevant Inactive Photosystem II Complex in Cyanobacteria

Cited by 34 publications

References 57 publications

Diel regulation of photosynthetic activity in the oceanic unicellular diazotrophic cyanobacterium Crocosphaera watsonii WH8501

Diel regulation of photosynthetic activity in the oceanic unicellular diazotrophic cyanobacterium Crocosphaera watsonii WH8501

Early Archean origin of Photosystem II

Early Archean origin of Photosystem II

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