Background: Painful experiences are common among hospitalized children. Long-term negative biopsychosocial consequences of under-treated pain are recognized. Aims: The study benchmarks pain prevalence, assessment, and treatments as a first step to improve pain care in a Canadian tertiary hospital. Methods: Single-day audits were undertaken on the pediatric ward (PW), emergency department (ED), and maternal services (MS). Participants (child or caregiverproxy) reported hospital pain experiences in the preceding 24 hours; medical records were reviewed for assessment and treatment. Results: Among 84 participants, pain prevalence ranged from 75-88%; mean pain intensity ranged from 5.7-6.5/10. Prevalence of moderate to severe pain was 78% on PW, 65% in ED, and 55% on MS. Needle pokes were the most frequent cause of worst pain. Documentation of pain assessment varied by setting (PW, 93%; ED, 13%; MS, 0%). Documented maximum pain scores were significantly lower compared to participant report (mean difference 4.5/10, SD=3.1, p<0.0001). A total 29% (6/21) of infants with heel lance or injection received breastfeeding or sucrose, and 29% (7/24) of participants receiving other needle procedures had documented or reported topical lidocaine use. All participants on MS underwent needle procedures. Conclusions: Pain is experienced commonly by infants and children in PW, ED, and MS. Pain assessment documentation is not routine and underestimates participant report. Evidence-based pain management strategies are underutilized. An institution-wide quality improvement approach is required to address pain care. Pain assessment and needle pain prevention and treatment should be prioritized in these pediatric acute care and newborn care settings.