An autoclavable glucose biosensor for microbial fermentation monitoring and control

Phelps, Michael R.; Hobbs, J. B.; Kilburn, Douglas G.; Turner, Robin F. B.

doi:10.1002/bit.260460604

Cited by 21 publications

(10 citation statements)

References 37 publications

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“…Phelps et al (156) addressed this problem based on the reversible immobilization of chemically conjugated CBM-glucose oxidase (CBM Cex from C. fimi), which can be repeatedly loaded onto a cellulose probe. Given that the binding is reversible, the sensor can be regenerated by replacing the originally bound enzyme with a fresh one (157,200). Shoseyov et al (177) developed a system based on CBMs that permits rapid detection of pathogenic microbes in food samples.…”

Section: Cbms As Analytical Tools In Research and Diagnosticsmentioning

confidence: 99%

Carbohydrate Binding Modules: Biochemical Properties and Novel Applications

Shoseyov

Shani

Levy

2006

Microbiol Mol Biol Rev

498

347

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SUMMARY Polysaccharide-degrading microorganisms express a repertoire of hydrolytic enzymes that act in synergy on plant cell wall and other natural polysaccharides to elicit the degradation of often-recalcitrant substrates. These enzymes, particularly those that hydrolyze cellulose and hemicellulose, have a complex molecular architecture comprising discrete modules which are normally joined by relatively unstructured linker sequences. This structure is typically comprised of a catalytic module and one or more carbohydrate binding modules (CBMs) that bind to the polysaccharide. CBMs, by bringing the biocatalyst into intimate and prolonged association with its substrate, allow and promote catalysis. Based on their properties, CBMs are grouped into 43 families that display substantial variation in substrate specificity, along with other properties that make them a gold mine for biotechnologists who seek natural molecular “Velcro” for diverse and unusual applications. In this article, we review recent progress in the field of CBMs and provide an up-to-date summary of the latest developments in CBM applications.

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Section: Cbms As Analytical Tools In Research and Diagnosticsmentioning

confidence: 99%

Carbohydrate Binding Modules: Biochemical Properties and Novel Applications

Shoseyov

Shani

Levy

2006

Microbiol Mol Biol Rev

498

347

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“…Fortunately, due to modern developments in this field, a wide selection of methods are available [9,10], such as flow injection analysis [11], near infrared spectroscopy [12,13], mid-infrared spectroscopy [14][15][16], HPLC [6], inexpensive gas sensors [8], biosensors [17,7,18,19] and mass spectrometry [20].…”

Section: Introductionmentioning

confidence: 99%

Control of yeast fed-batch process through regulation of extracellular ethanol concentration

Cannizzaro

Valentinotti

Stockar

2004

Bioprocess Biosyst Eng

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At high growth rates, the biomass yield of baker's yeast (Saccharomyces cerevisiae) decreases due to the production of ethanol. For this reason, it is standard industrial practice to use a fed-batch process whereby the specific growth rate, mu, is fixed at a level below the point of ethanol production, i.e., mucrit. Optimally, growth should be maintained at mucrit, but in practice, this is difficult because mucrit is dependent upon strain and culture conditions. In this work, growth was maintained at a point just above mucrit by regulating ethanol concentration in the bioreactor. The models used for control design are shown, as are the experimental results obtained when this strategy was implemented. This technique should be applicable to all microorganisms that exhibit an "overflow" type metabolism.

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“…The implementation of the biosensor in the technological line is made usually in one of the following ways: the target substrate is directly-detected (in-situ) in the fermenter. The practical requirements for an in-situ biosensor, such as: sterilisation possibility, adequate measuring range, resistance to membrane fouling, have so far not been entirely met, which has precluded the widespread application of this approach [84]. Due to these requirements, measurements in an external flow stream or in on-line systems are more often applied.…”

Section: Enzyme-based Amperometric Biosensors For Monitoring In Diffementioning

confidence: 98%

“…The biosensor displayed an improved stability (4 days of continuous use) and extended working range (up to 20 g/I). Also, an autoclavable glucose biosensor was used to monitor in-situ the fed-batch fermentation of Escherichia coli [ 84]. the target substrate is sampled via a flow-injection system and is detected using an amperometric biosensor.…”

Section: Enzyme-based Amperometric Biosensors For Monitoring In Diffementioning

confidence: 99%

“…Biosensors proved to be the right tools and therefore have been also used to develop feedback control strategies [84][85][86][87][88][89][90]. The implementation of the biosensor in the technological line is made usually in one of the following ways: the target substrate is directly-detected (in-situ) in the fermenter.…”

Section: Enzyme-based Amperometric Biosensors For Monitoring In Diffementioning

confidence: 99%

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Amperometric Enzyme-Based Biosensors for Application in Food and Beverage Industry

Csöoregi

Gáspñr

Niculescu

et al. 2001

Physics and Chemistry Basis of Biotechnology

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SummaryContinuous, sensitive, selective, and reliable monitoring of a large variety of different compounds in various food and beverage samples is of increasing importance to assure a high-quality and tracing of any possible source of contamination of food and beverages. Most of the presently used classical analytical methods are often requiring expensive instrumentation, long analysis times and well-trained staff. Amperometric enzyme-based biosensors on the other hand have emerged in the last decade from basic science to useful tools with very promising application possibilities in food and beverage industry. Amperometric biosensors are in general highly selective, sensitive, relatively cheap, and easy to integrate into continuous analysis systems. A successful application of such sensors for industrial purposes, however, requires a sensor design, which satisfies the specific needs of monitoring the targeted analyte in the particular application, Since each individual application needs different operational conditions and sensor characteristics, it is obvious that biosensors have to be tailored for the particular case. The characteristics of the biosensors are depending on the used biorecognition element (enzyme), nature of signal transducer (electrode material) and the communication between these two elements (electron-transfer pathway).Therefore, the present chapter presents the different existing biosensor designs describing the possible electron-transfer pathways, discusses their advantages and disadvantages, and shows their possible application in food and beverage industry. Three practical examples are given describing biosensor designs developed in our laboratory, demonstrating their usefulness for industrial applications.

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An autoclavable glucose biosensor for microbial fermentation monitoring and control

Cited by 21 publications

References 37 publications

Carbohydrate Binding Modules: Biochemical Properties and Novel Applications

Carbohydrate Binding Modules: Biochemical Properties and Novel Applications

Control of yeast fed-batch process through regulation of extracellular ethanol concentration

Amperometric Enzyme-Based Biosensors for Application in Food and Beverage Industry

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