An autologous cell lysate extract from human embryonic stem cell (hESC) derived osteoblasts can enhance osteogenesis of hESC

Heng, Boon Chin; Toh, Wei Seong; Pereira, Barry P.; Tan, Bernard; Fu, Xin; Liu, Hua; Lü, Kai; Yeo, Jin-Fei; Cao, Tong

doi:10.1016/j.tice.2007.12.003

Cited by 22 publications

(12 citation statements)

References 23 publications

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“…The cell-cell interactions and BMPs secreted by primary bone-derived cells stimulated human ESCs (hESCs) into osteogenic lineages in a direct co-culture system [87]. Cell extracts derived from hESC-derived osteogenic cultures induced undifferentiated hESCs into osteogenic lineage [88]. Human ESC-derived embryoid body cells were cultured in the presence of osteogenic supplements such as ascorbic acid and b-glycerophosphate (BGP) for 14 days, and dexamethasone was added to this medium for another 24 h. The stimulated cells were further seeded onto poly(lactic acid) (PLA) scaffolds and implanted subcutaneously to the back of immunodeficient mice for 5 weeks.…”

Section: Embryonic Stem Cells On Nanostructures For Bone Regenerationmentioning

confidence: 99%

Stem Cells and Nanostructures for Advanced Tissue Regeneration

Prabhakaran

Venugopal

Ghasemi‐Mobarakeh

et al. 2011

Biomedical Applications of Polymeric Nanofibers

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Stem cells are a promising alternative for cell therapy applications because of their self-renewing capability and potential to differentiate into a wide range of specialized cell lineages, including osteoblasts, chondrocytes, cardiomyocytes, and neuronal cells. Different kinds of stem cells are considered for cell-based tissue engineering approaches, of which bone-marrow-derived mesenchymal stem cells (BM-MSCs), adipose-derived stem cells (ADSCs) and embryonic stem cells (ESCs) are frequently utilized for advancement of new tissue engineering strategies. Nanostructures created by natural, synthetic, or composite polymeric biomaterials provide artificial templates of the extracellular matrix (ECM). They possess a high surface area to volume ratio, are porous with good mechanical properties, are sufficient to serve as a biomimetic platform to attract stem cells, cause differentiation, and provide functioning of the tissues. Nanoscale features such as fibers, pits, and grooves modulate cell behavior and might even stimulate the differentiation of stem cells to specific lineages; for example, the nanotopographical cues combined with chemical cues influence the neuronal induction of MSCs, resulting in high microtubule-associated protein expressions. Scaffolds can also be impregnated with several ligands for adhesion of receptors to the cells, or with other regulatory molecules and growth M.P. Prabhakaran (*) and J. Venugopal E3-05-12, Health Care and Energy Materials Laboratory, Nanoscience and Nanotechnology Initiative, Faculty of Engineering, National University of Singapore, 2 Engineering Drive 3, Singapore 117576, Singapore e-mail: nnimpp@nus.edu.sg L. Ghasemi-Mobarakeh Islamic Azad University, Najafabad Branch, Isfahan, Iran D. Kai NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, 2 Engineering Drive 3, Singapore 117576, Singapore G. Jin and S. Ramakrishna Department of Mechanical Engineering, National University of Singapore, 2 Engineering Drive 3, Singapore 117576, Singapore factors for designed cell behavior or controlled cell differentiations. Different polymeric blends or fibers incorporated with nanoparticles (such as hydroxyapatite) in aligned or custom-made patterns can induce specific differentiation of stem cells for bone, cartilage, cardiac, nerve, and skin tissue regeneration. In this review, we will discuss the current state of the art and future perspectives on stem cells and their differentiation on nanoengineered substrates for advanced tissue regeneration.

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Section: Embryonic Stem Cells On Nanostructures For Bone Regenerationmentioning

confidence: 99%

Stem Cells and Nanostructures for Advanced Tissue Regeneration

Prabhakaran

Venugopal

Ghasemi‐Mobarakeh

et al. 2011

Biomedical Applications of Polymeric Nanofibers

View full text Add to dashboard Cite

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“…In contrast, commercially available bone chips from human donors typically undergo sterilization many hours or even days later (33). Under such conditions, bone cells would no longer be vital, and it is possible that processing destroyed osteocytes embedded in bone that otherwise might contribute to the paracrine activity of the tissue (34). Therefore, the effect of time on paracrine-like activity is unclear.…”

Section: Discussionmentioning

confidence: 99%

High-dose irradiation of bone chips preserves the <i>in vitro</i> activity of bone-conditioned medium

et al. 2016

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Extracorporeal irradiation sterilizesresected tumor bone used as autografts in reconstruction surgery. Therapeutic irradiation is a standard technique in head and neck cancer therapy that aims to preserve organ function. Bone irradiation has a complex, mostly inhibitory, effect on remodeling and regeneration, although the underlying mechanisms are still not fully understood. It remains unclear if extracorporeal irradiation affects the paracrine-like activity of the corresponding autografts. We recently reported that bone-conditioned medium from autogenous bone chips contains a number of factors that might affect cell activity. In the present study, we investigated the effects of extracorporeal irradiation of porcine cortical bone chips on the activity of the corresponding bone-conditioned medium. The effects of bone-conditioned medium on the expressions of transforming growth factor-beta (TGF-β) target genes in oral fibroblasts were assessed. Bone-conditioned medium from bone chips exposed to a total radiation dose up to 120 Gy did not affect expressions of TGF-β target genes, including adrenomedullin, BTB/ POZ domain-containing protein 11, proteoglycan 4, NADPH oxidase 4, and interleukin 11, in oral fibroblasts. In conclusion, bone irradiation does not alter the capability of the corresponding bone-conditioned medium to provoke a robust fibroblastic cell response in vitro. (J Oral Sci 58, 325-331, 2016)

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“…An interesting alternative to traditional co-culture techniques involves culturing hESCs with an autologous osteogenic-inducing culture supplement extracted from hESC-derived osteogenic cells [100]. This novel approach was devised in order to overcome the cost associated with large-scale bioreactor cultures for commercial applications, as the resultant cost of supplementing cultures with growth factors, cytokines and extracellular matrix (ECM) would prove to be prohibitively expensive.…”

Section: Osteogenesis In Co-culturementioning

confidence: 99%

Augmentation of Musculoskeletal Regeneration: Role for Pluripotent Stem Cells

2018

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The rise in the incidence of musculoskeletal diseases is attributed to an increasing ageing population. The debilitating effects of musculoskeletal diseases, coupled with a lack of effective therapies, contribute to huge financial strains on healthcare systems. The focus of regenerative medicine has shifted to pluripotent stem cells (PSCs), namely, human embryonic stem cells and human-induced PSCs, due to the limited success of adult stem cell-based interventions. PSCs constitute a valuable cell source for musculoskeletal regeneration due to their capacity for unlimited self-renewal, ability to differentiate into all cell lineages of the three germ layers and perceived immunoprivileged characteristics. This review summarizes methods for chondrogenic, osteogenic, myogenic and adipogenic differentiation of PSCs and their potential for therapeutic applications.

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An autologous cell lysate extract from human embryonic stem cell (hESC) derived osteoblasts can enhance osteogenesis of hESC

Cited by 22 publications

References 23 publications

Stem Cells and Nanostructures for Advanced Tissue Regeneration

Stem Cells and Nanostructures for Advanced Tissue Regeneration

High-dose irradiation of bone chips preserves the <i>in vitro</i> activity of bone-conditioned medium

Augmentation of Musculoskeletal Regeneration: Role for Pluripotent Stem Cells

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