An Automated Algorithm to Quantify Collagen Distribution in Aortic Wall

Nguyen, Dustin M; Wagenhäuser, Markus U.; Mehrkens, Dennis; Adam, Matti; Tsao, Philip S.; Ramasubramanian, Anand K.

doi:10.1369/0022155418814231

Cited by 7 publications

(8 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Collagen content was quantified as the area of red of each image. The green channel of RGB images from picrosirius-red-stained lung sections was binarized with MATLAB software as previously reported 50 to quantify the infiltrates. Black pixels were counted, and the density was shown as pixel count per image.…”

Section: Methodsmentioning

confidence: 99%

Impaired ketogenesis ties metabolism to T cell dysfunction in COVID-19

Karagiannis

Peukert²,

Surace³

et al. 2022

Nature

View full text Add to dashboard Cite

This is a PDF file of a peer-reviewed paper that has been accepted for publication. Although unedited, the content has been subjected to preliminary formatting. Nature is providing this early version of the typeset paper as a service to our authors and readers. The text and figures will undergo copyediting and a proof review before the paper is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers apply.

show abstract

Section: Methodsmentioning

confidence: 99%

Impaired ketogenesis ties metabolism to T cell dysfunction in COVID-19

Karagiannis

Peukert²,

Surace³

et al. 2022

Nature

View full text Add to dashboard Cite

show abstract

“…Collagens actively participate in arterial wall remodeling during atherogenesis. It is well-studied and documented in the literature that local variations in fibrillar collagen are critical to arterial stiffening and the progression of AS ( 26 ). In our study, the increasing and redistribution of COL I and COL III were observed in the human carotid AS tissue.…”

Section: Discussionmentioning

confidence: 99%

“…(B) COL I, COL III, VCAM-1, ICAM-1, and p-JNK protein expression was determined by Western blot in the CAVSMCs treated with SP600125 (*p < 0.05 and **p < 0.01 vs. control group, # p < 0.05, ## p < 0.01, and ### p < 0.001 si-PRDX2+SP group, && p < 0.01, &&& p < 0.001, and &&&& p < 0.0001 vs. si-PRDX2-NC+SP group). (C) COL I, COL III, VCAM-1, ICAM-1, and p-ERK protein expression was determined by Western blot in the CAVSMCs treated with PD98059 (*p < 0.05 and **p < 0.01 vs. control group, # p < 0.05, ## p < 0.01, and ### p < 0.001 vs. si-PRDX2+PD group, && p < 0.01, &&& p < 0.001, and &&&& p < 0.0001 vs. si-PRDX2-NC+PD group).AS(26). In our study, the increasing and redistribution of COL I and COL III were observed in the human carotid AS tissue.…”

mentioning

confidence: 93%

PRDX2 Protects Against Atherosclerosis by Regulating the Phenotype and Function of the Vascular Smooth Muscle Cell

Wang

Liu

et al. 2021

Front. Cardiovasc. Med.

View full text Add to dashboard Cite

Peroxiredoxin 2 (PRDX2), an inhibitor of reactive oxygen species (ROS), is potentially involved in the progression of atherosclerosis (AS). The aim of this study was to explore the role and mechanism of PRDX2 in AS. The expression of PRDX2 was evaluated in 14 human carotid artery tissues with or without AS. The results showed that the positive reaction of PRDX2 was observed in the carotid artery vascular smooth muscle cells (CAVSMCs). To assess the mechanism by which PRDX2 may function in AS, the CAVSMCs were transfected with pEX4-PRDX2 and si-PRDX2. The catalase, hydrogen peroxide (H2O2) scavenger, was used to further confirm that PRDX2-induced inhibitory effects might be mediated through reducing ROS levels. Phenotype alteration and functional testing included transcription testing, immunostaining, and expression studies. The drug of MAPK signaling pathway inhibitors SB203580, SP600125, and PD98059 was used to evaluate the underlying mechanism. In this study, we found that the protein level of PRDX2 and the level of H2O2 were higher in the human AS carotid artery tissues than in the normal carotid artery tissues, accompanied with the activation of MAPK signaling pathway. The up-regulation of PRDX2 in the CAVSMCs significantly decreased the expression of ROS, collagen type I (COL I), collagen type III (COL III), vascular cell adhesion molecule-1 (VCAM-1), and intercellular adhesion molecule-1 (ICAM-1) and inhibited the proliferation, migration, and transformation of the CAVSMCs. The up-regulation of PRDX2 reversed the effect of the CAVSMCs treated with tumor necrosis factor-α (TNF-α). In addition, PRDX2 down-regulation promoted the protein levels of p-p38, p-JNK, and p-ERK, which was confirmed in relevant MAPK inhibitor treatment experiments. Our results suggest a protective role of PRDX2, as a scavenger of ROS, in AS progression through inhibiting the VSMC phenotype alteration and function via MAPK signaling pathway.

show abstract

“…Total collagen content of PSR stained sections was quantified by an elaborated analysis protocol using MatLab as described before [8]. PSR stained sections were imaged by a Leica microscope (Leica DM 4000B, Leica Biosystems Inc., USA) and a polarized filter (Leica ICT/Pol, Leica Biosystems Inc; Fig.…”

Section: Collagen Quantificationmentioning

confidence: 99%

“…The results obtained are compared to histological analyses and automated collagen quantification as described previously [8].…”

Section: Introductionmentioning

confidence: 99%

Vascular ultrasound forin vivoassessment of arterial pathologies in a murine model of atherosclerosis and aortic aneurysm

Hof

Guthoff

Ahdab

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Vascular diseases like atherosclerosis or aortic aneurysms are common pathologies in the western world, promoting various, potentially fatal conditions. Hence, a plethora of animal models have been developed to investigate underlying mechanisms and potential therapeutics. Here we evaluate high resolution (HR) ultrasound in mouse models of atherosclerosis and abdominal aortic aneurysm (AAA) for noninvasive monitoring of morphological and functional vascular changes in vivo. Methods: Eight-week-old ApoE-/- mice were used for disease models. For induction of atherosclerosis, mice were fed a western diet over 12 weeks. To trigger AAA development, osmotic minipumps were implanted, permanently releasing Angiotensin II continuously for 28 days. All animals were on C57Bl6/J background. HR vascular ultrasound of the carotid artery or the abdominal aorta was performed, respectively. Images obtained were analyzed by a speckle tracking algorithm (VevoVasc software) and were correlated with histological analyses by Picro Sirius Red staining and automated collagen quantification. Results: Arterial wall distensibility and global radial strain (GRS) as measures of arterial wall elasticity were reduced in the carotids of atherosclerotic mice as well as in the aortas of AAA mice. Pulse wave velocity (PWV) was elevated in both disease models. Intima-media thickness (IMT) was significantly increased in the atherosclerosis model. Matching those findings, area of the tunica media was enlarged in ApoE-/- mice fed a western diet, and in Angiotensin II treated mice as measured by automated image analysis, depicting higher collagen depositions in diseased arteries. Simple regression analysis revealed a strong correlation of media collagen content and area in AAA with IMT and GRS, respectively. In atherosclerosis, media collagen content significantly correlated with PWV and GRS, whereas wall distensibility was associated with the size of media area. Conclusion: Vascular imaging using latest generation HR ultrasound devices is suitable to trace changes of arterial wall properties in murine models of atherosclerosis and AAA. Obtained results not only correlate with histological findings but deliver information on functional parameters which may be used as early disease and risk markers in a longitudinal experimental approach.

show abstract

An Automated Algorithm to Quantify Collagen Distribution in Aortic Wall

Cited by 7 publications

References 15 publications

Impaired ketogenesis ties metabolism to T cell dysfunction in COVID-19

Impaired ketogenesis ties metabolism to T cell dysfunction in COVID-19

PRDX2 Protects Against Atherosclerosis by Regulating the Phenotype and Function of the Vascular Smooth Muscle Cell

Vascular ultrasound forin vivoassessment of arterial pathologies in a murine model of atherosclerosis and aortic aneurysm

Contact Info

Product

Resources

About