2024
DOI: 10.1021/acssynbio.4c00471
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An Automated Cell-Free Workflow for Transcription Factor Engineering

Holly M. Ekas,
Brenda Wang,
Adam D. Silverman
et al.

Abstract: The design and optimization of metabolic pathways, genetic systems, and engineered proteins rely on highthroughput assays to streamline design-build-test-learn cycles. However, assay development is a time-consuming and laborious process. Here, we create a generalizable approach for the tailored optimization of automated cell-free gene expression (CFE)-based workflows, which offers distinct advantages over in vivo assays in reaction flexibility, control, and time to data. Centered around designing highly accura… Show more

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Cited by 4 publications
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