An Automated High-Performance Liquid Chromatography Fluorescence Method for the Analyses of Endothelins in Plasma Samples

Kumarathasan, Prem; Goegan, Patrick; Vincent, Renaud

doi:10.1006/abio.2001.5362

Cited by 23 publications

(29 citation statements)

References 22 publications

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“…Blood was collected from the abdominal aorta into vacutainer tubes containing the sodium salt of EDTA at 10mg/ml and PMSF at 1.7mg/ml, mixed gently, and placed on ice (Kumarathasan et al ., 2001). Plasma was isolated by centrifugation (2000rpm for 10min), aliquoted, and frozen at −80°C.…”

Section: Methodsmentioning

confidence: 99%

Mapping Acute Systemic Effects of Inhaled Particulate Matter and Ozone: Multiorgan Gene Expression and Glucocorticoid Activity

Thomson

Vladisavljevic

Mohottalage

et al. 2013

Toxicological Sciences

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156

106

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Recent epidemiological studies have demonstrated associations between air pollution and adverse effects that extend beyond respiratory and cardiovascular disease, including low birth weight, appendicitis, stroke, and neurological/neurobehavioural outcomes (e.g., neurodegenerative disease, cognitive decline, depression, and suicide). To gain insight into mechanisms underlying such effects, we mapped gene profiles in the lungs, heart, liver, kidney, spleen, cerebral hemisphere, and pituitary of male Fischer-344 rats immediately and 24h after a 4-h exposure by inhalation to particulate matter (0, 5, and 50mg/m3 EHC-93 urban particles) and ozone (0, 0.4, and 0.8 ppm). Pollutant exposure provoked differential expression of genes involved in a number of pathways, including antioxidant response, xenobiotic metabolism, inflammatory signalling, and endothelial dysfunction. The mRNA profiles, while exhibiting some interorgan and pollutant-specific differences, were remarkably similar across organs for a set of genes, including increased expression of redox/glucocorticoid-sensitive genes and decreased expression of inflammatory genes, suggesting a possible hormonal effect. Pollutant exposure increased plasma levels of adrenocorticotropic hormone and the glucocorticoid corticosterone, confirming activation of the hypothalamic-pituitary-adrenal axis, and there was a corresponding increase in markers of glucocorticoid activity. Although effects were transient and presumably represent an adaptive response to acute exposure in these healthy animals, chronic activation and inappropriate regulation of the hypothalamic-pituitary-adrenal axis are associated with adverse neurobehavioral, metabolic, immune, developmental, and cardiovascular effects. The experimental data are consistent with epidemiological associations of air pollutants with extrapulmonary health outcomes and suggest a mechanism through which such health effects may be induced.

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Section: Methodsmentioning

confidence: 99%

Mapping Acute Systemic Effects of Inhaled Particulate Matter and Ozone: Multiorgan Gene Expression and Glucocorticoid Activity

Thomson

Vladisavljevic

Mohottalage

et al. 2013

Toxicological Sciences

Self Cite

156

106

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“…The remaining portion was transferred into a serum collection tube, left at room temperature for the collection of serum as described before [28]. Whole blood samples stabilized with preservatives (EDTA, PMSF) were centrifuged at 2000 rpm for 10 min to obtain plasma [30].…”

Section: Methodsmentioning

confidence: 99%

“…This procedure was conducted as described before [30]. Briefly, aliquoted plasma samples (250 µL) were treated with 3,4-dichloroisocoumarin solution in isopropanol to prevent conversion of big ET-1 to ET-1 during sample processing.…”

Section: Methodsmentioning

confidence: 99%

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Cardiovascular Changes in Atherosclerotic ApoE-Deficient Mice Exposed to Co60 (γ) Radiation

et al. 2013

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BackgroundThere is evidence for a role of ionizing radiation in cardiovascular diseases. The goal of this work was to identify changes in oxidative and nitrative stress pathways and the status of the endothelinergic system during progression of atherosclerosis in ApoE-deficient mice after single and repeated exposure to ionizing radiation.Methods and ResultsB6.129P2-ApoE tmlUnc mice on a low-fat diet were acutely exposed (whole body) to Co60 (γ) (single dose 0, 0.5, and 2 Gy) at a dose rate of 36.32 cGy/min, or repeatedly (cumulative dose 0 and 2 Gy) at a dose-rate of 0.1 cGy/min for 5 d/wk, over a period of 4 weeks. Biological endpoints were investigated after 3–6 months of recovery post-radiation. The nitrative stress marker 3-nitrotyrosine and the vasoregulator peptides endothelin-1 and endothelin-3 in plasma were increased (p<0.05) in a dose-dependent manner 3–6 months after acute or chronic exposure to radiation. The oxidative stress marker 8-isoprostane was not affected by radiation, while plasma 8-hydroxydeoxyguanosine and L-3,4-dihydroxyphenylalanine decreased (p<0.05) after treatment. At 2Gy radiation dose, serum cholesterol was increased (p = 0.008) relative to controls. Percent lesion area increased (p = 0.005) with age of animal, but not with radiation treatment.ConclusionsOur observations are consistent with persistent nitrative stress and activation of the endothelinergic system in ApoE−/− mice after low-level ionizing radiation exposures. These mechanisms are known factors in the progression of atherosclerosis and other cardiovascular diseases.

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“…HPLC analysis of plasma peptides was conducted as described by Kumarathasan et al [ 23 ]. Briefly, the proteins were precipitated in acid-acetone and subjected to molecular weight cut-off (30 kDa) filtration to retrieve peptides which were reconstituted in Dulbecco's phosphate-buffered saline (PBS) for HPLC separation.…”

Section: Methodsmentioning

confidence: 99%

Data mining of plasma peptide chromatograms for biomarkers of air contaminant exposures

2008

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An Automated High-Performance Liquid Chromatography Fluorescence Method for the Analyses of Endothelins in Plasma Samples

Cited by 23 publications

References 22 publications

Mapping Acute Systemic Effects of Inhaled Particulate Matter and Ozone: Multiorgan Gene Expression and Glucocorticoid Activity

Mapping Acute Systemic Effects of Inhaled Particulate Matter and Ozone: Multiorgan Gene Expression and Glucocorticoid Activity

Cardiovascular Changes in Atherosclerotic ApoE-Deficient Mice Exposed to Co60 (γ) Radiation

Data mining of plasma peptide chromatograms for biomarkers of air contaminant exposures

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