An automated multi-flume actograph for the study of behavioral rhythms of burrowing organisms

Sbragaglia, Valerio; Aguzzi, Jacopo; García, José A.; Sarriá, David; Gomáriz, S.; Costa, Corrado; Menesatti, Paolo; Vilaró, M.; Mànuel, A.; Sardà, Francesc

doi:10.1016/j.jembe.2013.05.018

Cited by 17 publications

(19 citation statements)

References 57 publications

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“…The algorithms applied can be found at (Sbragaglia et al, 2013) and produce as output the time series of displacements (cm over time) by each individual in the aquarium. The data shown marked emergence rhythms with respect to photoperiod (Figure 7) as well as the current cycle (Figure 8).…”

Section: Resultsmentioning

confidence: 99%

“…The burrows are built with PVC pipes with shape and volume similar to those found in nature (Sbragaglia et al, 2013). Each channel has two openings, through which water is recirculated by a pump (recirculation pipe in Figure 1).…”

Section: Aquariums and Specifications Habitatmentioning

confidence: 99%

“…The activity of the species is monitored using video cameras. Machine vision algorithms are being developed to identify individuals automatically when they emerge, and to measure the distance travelled outside the burrow (Sbragaglia et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Light and current generation system for measuring the behaviour of the Norway lobster

et al. 2015

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Patterns of how the Norway lobster (Nephrops norvegicus L.) emerges from its burrow in relation to sea currents at different times of the day-night cycles is currently unknown. The degree of influence of these variables when simulated in controlled laboratory conditions entails great technical and technological difficulties. This paper presents the development of an integrated control system that manages a set of aquariums generating water currents and patterns of light.The solution proposed combines the use of commercial equipment and a custom electronic design in order to achieve the required specifications with costminimization. The control system has circulation pumps and visible and infrared LED lighting to recreate different currents and light conditions. Preliminary results show the control system works properly and all components responded as expected during the experiments.Keywords: Wireless control, hydrodynamic aquarium regulation, lighting control, variable frequency drive, microcontroller Highlights: The electronic system implemented allows the study of marine species chronobiology. The system consists of a custom electronic board, circulation pumps and LED lighting. The configurable water flow and light conditions recreate different sea bed scenarios. Wireless interfaces reduce cost and installation complexity in aquariums. The infrared lighting enables the study of species in the dark with cameras.

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Section: Resultsmentioning

confidence: 99%

Section: Aquariums and Specifications Habitatmentioning

confidence: 99%

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Light and current generation system for measuring the behaviour of the Norway lobster

et al. 2015

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“…An actograph was used to track Nephrops norvegicus behaviour (Sbragaglia et al 2013a). Briefly, the actograph consisted of four tanks, each of which had two individual corridors (150 × 25 × 30 cm), in which the burrow emergence behaviour of lobsters was tracked by automated video image analysis.…”

Section: The Actographmentioning

confidence: 99%

“…The frames were automatically processed by a set of MAT-LAB functions, and time series of locomotor activity (cm) were obtained; for more information see Sbragaglia et al (2013a). Moreover, all of the frames were assembled into a time-lapse video (hereafter referred to as the full-length video) for further characterization of lobster behaviour (see below).…”

Section: The Actographmentioning

confidence: 99%

Effect of simulated tidal currents on the burrow emergence rhythms of the Norway lobster (Nephrops norvegicus)

et al. 2015

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time orientated downstream during darkness hours. Moreover, four of the 15 lobsters showed that a current could act as a putative zeitgeber for the circadian oscillator, but further experiments are needed to confirm this finding. These results indicate that tidal current is an important parameter to consider when interpreting fishery-dependent data and data from video surveys, not only N. norvegicus, but for other deep-water epibenthic species.

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Burrow emergence rhythms of deep-water Mediterranean Norway lobsters (Nephrops norvegicus) revealed by acoustic telemetry

Aguzzi

Vigo

Bahamón

et al. 2023

Rev Fish Biol Fisheries

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N. norvegicus supports one of the most commercially-important fisheries in the Mediterranean, and there is considerable interest in developing non-invasive sampling stock assessment methods. Currently, stock assessments are conducted via trawling or by UnderWater TeleVision (UWTV) surveys with limited capacity to provide direct population data due to the burrowing behavior of the species. Here, we used acoustic telemetry to characterize the burrow emergence and movement patterns of N. norvegicus in relation to internal tides and inertial currents in deep-sea habitats of the northwestern Mediterranean. We deployed acoustic tags on 25 adults between May and June 2019, and collected up to 4 months of detection data from each tag. Tagged lobsters spent approximately 12% of their time in door-keeping (i.e., detections at burrow entrance with no displacements) but differences existed among the different behavioral rhythms identified. We observed that mixed day-night, tidal and inertial rhythms in field settings were similar to those observed in laboratory tests. The presence of mixed day-night and tidal periodicity poses the question of why N. norvegicus exhibits flexible responses to environmental cues other than photoperiod. It is possible that tidal regulation of locomotor activity could reduce energy expenditure in relation to hydrodynamic drag. Inertial periodicity occurs in animals with no clear burrowing-oriented activity (always present on the seabed). Possibly, inertial-related movements are the result of a disruption of the biological clock. Our results are discussed in the context of how burrow emergence rhythms may bias UWTV surveys and how novel in situ monitoring approaches address these biases.

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An automated multi-flume actograph for the study of behavioral rhythms of burrowing organisms

Cited by 17 publications

References 57 publications

Light and current generation system for measuring the behaviour of the Norway lobster

Light and current generation system for measuring the behaviour of the Norway lobster

Effect of simulated tidal currents on the burrow emergence rhythms of the Norway lobster (Nephrops norvegicus)

Burrow emergence rhythms of deep-water Mediterranean Norway lobsters (Nephrops norvegicus) revealed by acoustic telemetry

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