An automated nanoliter dispenser for staining individual biopsies in tissue microarrays

Slobodan, Jared R.; Gunn, Dylan; Thompson, Jason; Guggenheimer, Kurtis; Brown, Keddie; Homenuke, Mark; Belak, Roy; Banerjee, Diponkar; Broemeling, David; Marziali, Andre

doi:10.3892/ijo.30.4.857

Cited by 1 publication

(4 citation statements)

References 4 publications

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“…Testing with the first pin design has shown the cleaning method to be sufficient for preventing crosstalk between runs. 6…”

Section: Analysis Of Pin Operationmentioning

confidence: 99%

“…This pin is mounted into a custom-built electrical socket attached to the tri-axis linear motion system of the TMAS instrument. 6 Three different reagents were used in the tests described:…”

Section: Methodsmentioning

confidence: 99%

“…2) capable of delivering reagent to individual biopsies within a TMA. 6 This offers two principal advantages. By delivering reagents to individual biopsies, it is possible to perform multiplexed tests on a single slide, and a TMA formed from cores of a patient's tumor biopsy, or a cell suspension array of fine needle aspirations, could be tested for a variety of biomarkers to obtain a complete cancer profile of the patient, all on one slide.…”

Section: Introductionmentioning

confidence: 99%

“…Testing with the first pin design has shown the cleaning method to be sufficient for preventing crosstalk between runs. 6 Although the staining results shown (Fig. 9) were achieved using the first pin design (Fig.…”

Section: Analysis Of Pin Operationmentioning

confidence: 99%

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A Novel Applicator Design for Contact-Free Delivery of Aqueous Reagent to Individual Biopsies in a Tissue Microarray

Gunn

Slobodan

Kamran

et al. 2007

JALA: Journal of the Association for Laboratory Automation

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The use of molecular techniques to inform diagnosis, prognosis, and treatment design will play an important role in the future of medicine. Each new test, however, represents a new cost to the health care system, and significant effort is required to move new techniques to the clinical setting in the most cost-effective and efficient manner. Consequently, there is a compelling need for technological improvements that will facilitate clinical application of novel assays, by reducing cost and complexity in implementation. This is particularly important in cancer pathology. Here, we present a novel applicator technology that enables staining of individual biopsies in a tissue microarray (TMA) to provide low-cost, multiplexed biomarker testing at the level of intact tissue. The applicator is designed to deliver tens of nanoliters of aqueous reagent to arrayed tissue biopsies kept under a layer of oil-based Liquid Coverslip without contacting the biopsies. A pin consisting of concentric stainless steel electrodes separated by a hydrophobic insulator provides a balance between hydrophobicity and hydrophilicity to hold a reagent droplet on the tip of the pin, whereas a small electrical current passed through the droplet spanning the electrodes is used for drop sensing. This design is more amenable to repeatable manufacturing than a previous prototype, which in initial testing demonstrated successful immunohistochemical and in situ hybridization staining of individual biopsies in a TMA, but was difficult to produce. This new design was tested to investigate the factors affecting its operation, in terms of the volume of reagent picked up and its ability to successfully deliver reagent to the biopsies.

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“…Testing with the first pin design has shown the cleaning method to be sufficient for preventing crosstalk between runs. 6…”

Section: Analysis Of Pin Operationmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Analysis Of Pin Operationmentioning

confidence: 99%

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