An Automated Procedure for the Determination of Total Amino Acids and its Application to the Analysis of Tobacco

Collins, PM; Sarji, N.M.; Williams, John F

doi:10.2478/cttr-2013-0335

Cited by 1 publication

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“…The level of amino acids in tobacco is an important parameter for leaf characterization, both related to tobacco quality (1)(2)(3) and also related to the generation of toxicants in cigarette smoke (4)(5). The smoke sensory properties are affected by the amino acids either directly, or through the further formation of Amadori compounds (1).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Content of Free Amino Acids in Tobacco by a New Liquid Chromatography-Tandem Mass Spectrometry Technique

Moldoveanu

Zhu

Scott

2015

Beiträge Zur Tabakforschung International/Contributions to Tobacco Research

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SummaryThe present study describes a reliable technique for the analysis of free amino acids in tobacco leaf. The levels of amino acids in tobacco are important since they are related to both tobacco quality and the potential generation in cigarette smoke of toxicants having amino acid precursors. Other techniques used in the past for amino acid analysis have various shortcomings that were avoided in the present method. The new method uses HPLC separation and a tandem mass spectrometer for detection with no derivatization step as sample preparation. The separation has been obtained using ion pair HPLC on a reversed phase column that offers excellent chromatographic resolution. The MS/MS detection procedure offers very good sensitivity and positive identification of the analytes. The procedure was fully validated and can be used for the analysis of 24 amino acids. It was applied for the quantitation of amino acids from 16 types of tobacco including flue-cured and Burley, some domestic and some not grown in the USA, two types of Oriental tobacco, and from tobacco of a 3R4F Kentucky reference and a common commercial cigarette. It was shown that the analysis provides useful information regarding the amino acid level variation between tobacco types, between tobacco stalk positions, and between the growing locations of different tobaccos. [Beitr. Tabakforsch. Int. 26 (2015) 334-343]

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Section: Introductionmentioning

confidence: 99%

Evaluation of the Content of Free Amino Acids in Tobacco by a New Liquid Chromatography-Tandem Mass Spectrometry Technique

Moldoveanu

Zhu

Scott

2015

Beiträge Zur Tabakforschung International/Contributions to Tobacco Research

View full text Add to dashboard Cite

show abstract

An Automated Procedure for the Determination of Total Amino Acids and its Application to the Analysis of Tobacco

Abstract: An automated procedure for the determination of total alpha-amino acids in aqueous solution has been developed which utilizes the Technicon Auto Analyser I. The procedure is based in part on a manual method proposed by Van Slyke wherein the CO

Cited by 1 publication

References 4 publications

Evaluation of the Content of Free Amino Acids in Tobacco by a New Liquid Chromatography-Tandem Mass Spectrometry Technique

Evaluation of the Content of Free Amino Acids in Tobacco by a New Liquid Chromatography-Tandem Mass Spectrometry Technique

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