An Automated Single‐Cell Droplet‐Digital Microfluidic Platform for Monoclonal Antibody Discovery

Ahmadi, Fatemeh; Tran, Hao; Letourneau, Natasha; Little, Samuel R.; Fortin, Annie; Moraitis, Anna N.; Shih, Steve C. C.

doi:10.1002/smll.202308950

Cited by 3 publications

(1 citation statement)

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“…Since the traps are not typically isolated, another challenge is the high chance of cell content contamination. Recently, a method for trapping cell-encapsulating droplets has been introduced, answering most of the mentioned drawbacks of the old systems [28]. In this system, the cells do not experience high shear stresses, and the droplet acts as an isolated microchamber, preventing unwanted crosscontaminations.…”

Section: Passive Hydrodynamic Trap-based Microfluidicsmentioning

confidence: 99%

Single-Cell RNA Sequencing in Organ and Cell Transplantation

Abedini-Nassab,

Taheri,

Emamgholizadeh

et al. 2024

Biosensors

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Single-cell RNA sequencing is a high-throughput novel method that provides transcriptional profiling of individual cells within biological samples. This method typically uses microfluidics systems to uncover the complex intercellular communication networks and biological pathways buried within highly heterogeneous cell populations in tissues. One important application of this technology sits in the fields of organ and stem cell transplantation, where complications such as graft rejection and other post-transplantation life-threatening issues may occur. In this review, we first focus on research in which single-cell RNA sequencing is used to study the transcriptional profile of transplanted tissues. This technology enables the analysis of the donor and recipient cells and identifies cell types and states associated with transplant complications and pathologies. We also review the use of single-cell RNA sequencing in stem cell implantation. This method enables studying the heterogeneity of normal and pathological stem cells and the heterogeneity in cell populations. With their remarkably rapid pace, the single-cell RNA sequencing methodologies will potentially result in breakthroughs in clinical transplantation in the coming years.

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Section: Passive Hydrodynamic Trap-based Microfluidicsmentioning

confidence: 99%

Single-Cell RNA Sequencing in Organ and Cell Transplantation

Abedini-Nassab,

Taheri,

Emamgholizadeh

et al. 2024

Biosensors

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A Microfluidic Multiplex Sorter for Strain Development

Leal‐Alves,

Dumont,

Deng

et al. 2024

Adv Materials Technologies

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Selecting strains with superior traits from strain improvement strategies is challenging, as it involves navigating the fitness landscape by applying selective pressures that drive variants from peaks of improvement to valleys over time. In recent years, the screening and selection is conducted via droplet microfluidic methods due to its high throughput capabilities. However, the oft‐used binary strategy, targeting only the high levels of improved traits, may not reflect the overall enhancement. A multiplexed sorting method capable of applying an additional threshold to sort traits by phenotypic strength is reported. The novel approach uses a droplet‐digital microfluidic sorter to screen different volumes of droplets using the same device design and sorting parameters. This method is used to sort glucoamylase enzyme mutants with two levels of activity (medium and high) from libraries of diastatic yeast that have been mutated with non‐genetically modified techniques. Using the multiplex system, medium‐performing strains with enhanced (up to 60%) fermentation kinetics in synthetic beverage media, which would have been missed with a binary screening approach, are identified. The multiplex sorting strategy efficiently finds strains with superior fermentation traits in the fitness landscape without requiring extensive screening rounds and mutations.

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