An automated system for CE-MALDI and on-target digestion under a fluorocarbon lid applied on spermatophore proteins from Pieris napi

Romson, Joakim; Jacksén, Johan; Emmer, Åsa

doi:10.1016/j.jchromb.2018.11.021

Cited by 7 publications

(6 citation statements)

References 40 publications

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“…It allows the exact determination of M r with an accuracy of ±0.1% and identification of peptides and proteins isolated by micropreparative CZE, see section 7.2. The automated system for offline CE‐MALDI‐TOF‐MS and MS/MS and on‐target tryptic digestion under fluorocarbon lid was developed using a home‐made robotic system [186]. The system was employed for analysis of spermatophore proteins of the butterfly Pieris napi .…”

Section: Detectionmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2019–mid 2021)

Kašička

2021

Electrophoresis

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The review provides a comprehensive overview of developments and applications of high performance capillary and microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) for analysis, microscale isolation, and physicochemical characterization of peptides from 2019 up to approximately the middle of 2021. Advances in the investigation of electromigration properties of peptides and in the methodology of their analysis, such as sample preparation, sorption suppression, EOF control, and detection, are presented. New developments in the individual CE and CEC methods are demonstrated and several types of their applications are shown. They include qualitative and quantitative analysis, determination in complex biomatrices, monitoring of chemical and enzymatic reactions and physicochemical changes, amino acid, sequence, and chiral analyses, and peptide mapping of proteins. In addition, micropreparative separations and determination of significant physicochemical parameters of peptides by CE and CEC methods are described.

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Section: Detectionmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2019–mid 2021)

Kašička

2021

Electrophoresis

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“…Off-line CE-MALDI-TOF-MS and MS/MS were employed for the analysis of proteins in the spermatophore of the butterfly Pieris Napi [104]. CE separation was performed in BGE composed of 20 mM KH 2 PO 4 , pH 2.31 (adjusted with H 3 PO 4 ), and 150 μg/ml Fluorad FC-134 fluorosurfactant for dynamic capillary coating.…”

Section: Ce Coupled With Maldi-msmentioning

confidence: 99%

Recent developments and applications of capillary and microchip electrophoresis in proteomics and peptidomics (mid‐2018–2022)

Štěpánová

Kašička

2023

J of Separation Science

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This review gives a wide overview of recent advances and applications of capillary electrophoresis and microchip capillary electrophoresis methods in the fields of proteomics and peptidomics in the period from mid‐2018 up to the end of 2022. The methodological topics covering sample preparation and concentration techniques, hyphenation of capillary electrophoresis methods with mass spectrometry, and multidimensional separations by on‐line or off‐line coupled different capillary electrophoresis and liquid chromatography techniques are described and new developments in both bottom‐up and top‐down approaches in proteomics are presented. In addition, various applications of capillary electrophoresis methods in proteomic and peptidomic studies are demonstrated. They include monitoring of protein posttranslational modifications and applications in biological and biochemical research, clinical peptidomics and proteomics, and food analysis.

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“…Off-line approaches have also allowed taking some advantage of advanced oxidation methods (combining H 2 O 2 and UV light) to follow the degradation of pharmaceuticals [529] or to convert alcohols into the corresponding acids, thus facilitating their separation by CE [350,530]. In order to avoid evaporation of the samples during pretreatment, Romson et al used an FC lid [531], a simple and clever approach that uses FC-770 Fluorinert (an inert, fully fluorinated liquid used in many instruments as coolant, δ = 1.79 g/mL). This off-line approach was used to incorporate trypsin digestion (2 h at room temperature) and identify sequence homologies in spermatophore proteins from Pieris napi.…”

Section: Derivatizationmentioning

confidence: 99%

“…In order to avoid evaporation of the samples during pretreatment, Romson et al. used an FC lid [531], a simple and clever approach that uses FC‐770 Fluorinert (an inert, fully fluorinated liquid used in many instruments as coolant, δ = 1.79 g/mL). This off‐line approach was used to incorporate trypsin digestion (2 h at room temperature) and identify sequence homologies in spermatophore proteins from Pieris napi .…”

Section: Direct Injection Sample Pretreatment and Preconcentrationmentioning

confidence: 99%

Strategies for capillary electrophoresis: Method development and validation for pharmaceutical and biological applications—Updated and completely revised edition

et al. 2023

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This review is in support of the development of selective, precise, fast, and validated capillary electrophoresis (CE) methods. It follows up a similar article from 1998, Wätzig H, Degenhardt M, Kunkel A. “Strategies for capillary electrophoresis: method development and validation for pharmaceutical and biological applications,” pointing out which fundamentals are still valid and at the same time showing the enormous achievements in the last 25 years. The structures of both reviews are widely similar, in order to facilitate their simultaneous use. Focusing on pharmaceutical and biological applications, the successful use of CE is now demonstrated by more than 600 carefully selected references. Many of those are recent reviews; therefore, a significant overview about the field is provided. There are extra sections about sample pretreatment related to CE and microchip CE, and a completely revised section about method development for protein analytes and biomolecules in general. The general strategies for method development are summed up with regard to selectivity, efficiency, precision, analysis time, limit of detection, sample pretreatment requirements, and validation.

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An automated system for CE-MALDI and on-target digestion under a fluorocarbon lid applied on spermatophore proteins from Pieris napi

Cited by 7 publications

References 40 publications

Recent developments in capillary and microchip electroseparations of peptides (2019–mid 2021)

Recent developments in capillary and microchip electroseparations of peptides (2019–mid 2021)

Recent developments and applications of capillary and microchip electrophoresis in proteomics and peptidomics (mid‐2018–2022)

Strategies for capillary electrophoresis: Method development and validation for pharmaceutical and biological applications—Updated and completely revised edition

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