An automated system for predicting detection limit and precision profile from a chromatogram

Kotani, Akio; Tsugu, Saeko; Hakamata, Hideki; Hayashi, Yuzuru

doi:10.1016/j.chroma.2019.460644

Cited by 9 publications

(2 citation statements)

References 22 publications

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“…The aliquot of the extracted test sample was injected into the HPLC to obtain a curve pending peak area and profile in a chromatogram. Then the peak area of the sample is compared with that of the standard, relative to the concentration of the standard to obtain the concentration of the sample, the following chromatographic conditions were employed; Agilent Technologies HPLC 1200, Column: uBondapak C18, Carrier: Acetonitrile/Water 70:30 and 5µL injected @ 2ml / min flow rate (Akira et al, 2020).…”

Section: Methodsmentioning

confidence: 99%

Identification of ascorbic acid and phytochemical profiles of Scotch Bonnet pepper (Capsicum chinense) using High-Performance Liquid Chromatography-Ultraviolet Detector (HPLC-UV)

Ezekiel,

Abiodun,

Funmilayo

et al. 2023

JANS

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Researchers have discovered that red and green scotch peppers are extremely rich in phytochemicals and that these secondary metabolites are connected to several biological processes in the human body. This study aimed to determine the numerous phytochemicals present in scotch pepper seeds, flesh, and red and green varieties. The samples were pulverized after being air-dried. The phytochemicals and ascorbic acid contents were examined using a High-performance Liquid Chromatography-Ultraviolet detector (HPLC-UV). Twelve phytochemicals and ascorbic acid were identified in the flesh and seed of red scotch pepper, with lycopene having the highest content (83.10 mg/kg), the ascorbic acid content of 8.80 mg/kg; moreover, the seed of red scotch pepper revealed twelve phytochemicals and lycopene being the highest content (75.60 mg/kg) and ascorbic acid content of 6.80 mg/kg, furthermore, fourteen phytochemicals were identified in the flesh of red scotch bonnet pepper with lycopene the highest quantity (86.00 mg/kg) and ascorbic content of 10.60 mg/kg. Twelve phytochemicals were identified in the flesh and seed of green scotch bonnet pepper, lycopene (68.70 mg/kg) being the highest content, ascorbic acid content (6.50 mg/kg), twelve phytochemicals were revealed in the flesh of green pepper and lycopene had highest quantity (76.30 mg/kg), in the seed of green pepper, the lycopene content was 68.70 mg/kg. However, it has been shown that capsaicin, dihydrocapsaicin, and lutein concentrations varied, with red scotch pepper flesh having the highest concentration of capsaicin (7.50 mg/kg). The lycopene and ascorbic acid contents of flesh were the highest (86.00 mg/kg) and (10.60 mg/kg). The study's findings showed that red pepper flesh was better than other components that were looked at.

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Section: Methodsmentioning

confidence: 99%

Identification of ascorbic acid and phytochemical profiles of Scotch Bonnet pepper (Capsicum chinense) using High-Performance Liquid Chromatography-Ultraviolet Detector (HPLC-UV)

Ezekiel,

Abiodun,

Funmilayo

et al. 2023

JANS

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show abstract

“…All samples were analyzed in duplicate, while working solution standards were analyzed in triplicate. The detection (DL) and quantitation (QL) limits of the method were calculated based on the parameters for each constructed analytical curve, using the following mathematical relations [23,24]:…”

Section: Adsorbent Characterizationmentioning

confidence: 99%

Effect of calcination temperature on material from an ant colony (termite): searching for the characterization of new dye adsorbent materials

et al. 2022

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The reuse of contaminated water arising from industries is a major challenge in the environmental field, which enables the search for treatments with a view to sustainable development. In this context, the objective of this study was to evaluate the effect of the calcination material from an ant colony (termite) in the production of adsorbent materials for methylene blue dye removal. The natural material (NC) and calcined materials at temperatures of 290ºC (C-290), 380ºC (C-380), 450ºC (C-450) and 540ºC (C-540) were characterized using techniques of thermal analysis and Fourier Transform Infrared Spectroscopy (FTIR). In NC and C-290 samples, the volatile compounds were evaluated by HS-GC/MS and phenolic compounds were identified by HPLC. The results for the adsorption of the dye methylene blue showed that, while the adsorption process can not be quantified employing the natural material, the process efficiency was quite relevant when the sample was calcined at a temperature of 290°C, whose removal percentage was 96%. Although the calcined sample at this temperature is the most similar to the starting material, performed at a temperature of 290°C acted in reducing the complexity of phenolic and volatile compounds found in the NC sample, which may negatively impact in the adsorption process.

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An automated assessment system of limits of detection and quantitation in gradient high-performance liquid chromatography with ultraviolet detection

Kotani

Hakamata

Hayashi³

2020

Journal of Chromatography A

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An automated system for predicting detection limit and precision profile from a chromatogram

Cited by 9 publications

References 22 publications

Identification of ascorbic acid and phytochemical profiles of Scotch Bonnet pepper (Capsicum chinense) using High-Performance Liquid Chromatography-Ultraviolet Detector (HPLC-UV)

Identification of ascorbic acid and phytochemical profiles of Scotch Bonnet pepper (Capsicum chinense) using High-Performance Liquid Chromatography-Ultraviolet Detector (HPLC-UV)

Effect of calcination temperature on material from an ant colony (termite): searching for the characterization of new dye adsorbent materials

An automated assessment system of limits of detection and quantitation in gradient high-performance liquid chromatography with ultraviolet detection

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