This paper presents the collaboration between unmanned aerial vehicles (UAVs) and unmanned ground vehicles (UGVs) for site-specific application of chemical (herbicides, pesticides, and other chemicals). The paper shows and discusses the experimental plot used in the project, methods for collaboration between the UAVs and UGVs for detection and isolation of weeds and strawberry plants using machine learning and remote sensing techniques, and methods for sitespecific application of chemicals. The paper also discusses the method of communication between UAVs and UGVs for data sharing and hardware used in the project including UAVs, UGVs, sensors, and communication devices. Some experimental results are shown and discussed.