An autoradiographic study of neurotensin receptors in the human hypothalamus

Najimi, Mohamed; Sarrieau, Alain; Kopp, Nicolas; Chigr, Fatiha

doi:10.1016/j.acthis.2013.09.008

Cited by 7 publications

(10 citation statements)

References 42 publications

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“…Furthermore, individual kinetic affinities did not vary significantly, arguing for a stability of NT binding sites affinity during development. Interestingly, kinetics of NT binding, were equivalent to those we reported in adult hypothalamus (Najimi et al, 2014) suggesting that they are not age related. Such stability during postnatal development for NT binding sites has been reported previously in other human brain structures (Chigr et al, 1992; Zsürger et al, 1992).…”

Section: Discussionsupporting

confidence: 83%

“…We investigated in the present study, the autoradiographic distribution and quantitation of NT receptors in hypothalamus of postmortem normal human hypothalamus during development. This completes our previous study, analyzing the overall distribution of NT binding sites in the adult human hypothalamus (Najimi et al, 2014). To date, three NT receptors with distinct functional and pharmacological properties have been identified: NT1, NT2, and nts3 (for review, see Le et al, 1996; Vincent et al, 1999).…”

Section: Discussionsupporting

confidence: 81%

“…In accordance of this, previous studies in human brain with PMD’s up to 42 h (Palacios et al, 1991) or rat brain up to 48 h (Sadoul et al, 1984) showed no significant negative effect of PMD’s on NT binding. Furthermore, our previous studies in human did not relate any influence of this factor on NT receptor density (Sarrieau et al, 1985; Chigr et al, 1992; Najimi et al, 2014). Finally, the higher PMD of the sample investigated (34 h, case B) belong to the neonate group expressing high densities as well as for the lowest PMD (7 h, case J).…”

Section: Discussionmentioning

confidence: 80%

“…For the anatomical localization of the hypothalamic nuclei and areas, sections adjacent to those used for autoradiography were stained with cresyl violet. We adopted conventional nomenclature we used in our previous investigations (Najimi et al, 2001, 2006, 2014). …”

Section: Methodsmentioning

confidence: 99%

“…Thus, intracerebroventricular or local NT injections in hypothalamus have been associated with dramatic alterations of the plasma levels of most anterior pituitary hormones (Alexander and Leeman, 1992; Vijayan et al, 1994; Rostène and Alexander, 1997; Sicard et al, 2005). Of interest, the hypothalamus, as reported in laboratory animals and human is especially enriched in endogenous peptide and NT receptors (Sarrieau et al, 1985; Kanba et al, 1986; Mai et al, 1987; Sakamoto et al, 1987; Rostène and Alexander, 1997; Najimi et al, 2014). The human hypothalamus is not completely mature at birth and its maturation continues during the postnatal period with relation to the maturation of peptidergic factors regulating pituitary function (Swaab, 1995).…”

Section: Introductionmentioning

confidence: 99%

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Developmental dynamics of neurotensin binding sites in the human hypothalamus during the first postnatal year

Najimi

Sarrieau

Kopp

et al. 2014

Front. Cell. Neurosci.

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The aim of the present study was to determine a detailed mapping of neurotensin (NT) in the human hypothalamus, during the first postnatal year using an in vitro quantitative autoradiography technique and the selective radioligand monoiodo-Tyr3-NT. Ten human postmortem hypothalami obtained from control neonates and infants (aged from 2 h to 1 year of postnatal age) were used. The biochemical kinetics of the binding in all obtained in this study revealed that the binding affinity constants were of high affinity (in the nanomolar range) and did not differ significantly between all cases investigated. Furthermore, competition experiments show insensitivity to levocabastine and were in favor of the presence of the high affinity site of NT receptor. Autoradiographic distribution showed that NT binding sites were widely distributed throughout the rostrocaudal extent of the hypothalamus. However, the distribution of NT binding sites was not homogenous and regional variations exist. In general, the highest densities were mainly present in the anterior hypothalamic level, particularly in the preoptic area. High NT binding site densities are also present at the mediobasal hypothalamic level, particularly in the paraventricular, parafornical, and dorsomedial nuclei. At the posterior level, low to very low densities could be observed in all the mammillary complex subdivisions, as well as the posterior hypothalamic area. Although this topographical distribution is almost identical during the postnatal period analyzed, age-related variations exist in discrete structures of the hypothalamus. The densities were higher in neonates/less aged infants than older infants in preoptic area (medial and lateral parts). The developmental profile is characterized by a progressive decrease from the neonate period to 1 year of postnatal age with a tendency to reach adult levels. On the other hand, the low levels of NT binding sites observed in posterior hypothalamus did not vary during the first postnatal year. They contrast in that with the very high levels we reported previously in adult. In conclusion, the present study demonstrates the occurrence of high NT binding sites density in various structures in many regions in the human neonate/infant hypothalamus, involved in the control of neuroendocrine and/or neurovegetative functions.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 80%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Developmental dynamics of neurotensin binding sites in the human hypothalamus during the first postnatal year

Najimi

Sarrieau

Kopp

et al. 2014

Front. Cell. Neurosci.

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Neurochemistry of the mammillary body

Żakowski

Zawistowski

2023

Brain Struct Funct

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The mammillary body (MB) is a component of the extended hippocampal system and many studies have shown that its functions are vital for mnemonic processes. Together with other subcortical structures, such as the anterior thalamic nuclei and tegmental nuclei of Gudden, the MB plays a crucial role in the processing of spatial and working memory, as well as navigation in rats. The aim of this paper is to review the distribution of various substances in the MB of the rat, with a description of their possible physiological roles. The following groups of substances are reviewed: (1) classical neurotransmitters (glutamate and other excitatory transmitters, gamma-aminobutyric acid, acetylcholine, serotonin, and dopamine), (2) neuropeptides (enkephalins, substance P, cocaine- and amphetamine-regulated transcript, neurotensin, neuropeptide Y, somatostatin, orexins, and galanin), and (3) other substances (calcium-binding proteins and calcium sensor proteins). This detailed description of the chemical parcellation may facilitate a better understanding of the MB functions and its complex relations with other structures of the extended hippocampal system.

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