An cell-assembly derived physiological 3D model of the metabolic syndrome, based on adipose-derived stromal cells and a gelatin/alginate/fibrinogen matrix

Xu, Mingzhen; Wang, Xiaohong; Yan, Yongnian; Yao, Ri; Ge, Yakun

doi:10.1016/j.biomaterials.2010.01.111

Cited by 138 publications

(166 citation statements)

References 51 publications

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“…This makes the approach particularly well suited for the optimization of the test parameters. The small changes in sample composition across gradient samples have been found to affect cellular attachment [90,92,[94][95][96][97], viability [27,64,72], migration [66,69,70,[98][99][100] and differentiation [71][72][73]83,[101][102][103][104]. To demonstrate this phenomena, Figure 2 shows a study of the effects of a Young's modulus gradient on human chondrocyte glycosaminoglycan content.…”

Section: Gradient Samplesmentioning

confidence: 99%

“…Detection of these shifts in cellular behavior are easier in gradient samples than in DOE and arrays due to reduced sample preparation, which limits sample variation effect on the biological results. Due to the ease and flexibility of fabrication, a number of studies have utilized gradient hydrogel systems to examine the effect of material property changes spanning from studies of Young's modulus [11,66,70,83,92,96,97,99,101,[104][105][106] to bioactive signal concentration [19,22,[76][77][78][79]90,94,95,100,102,107] on cellular behavior with numerous cell types. Large changes in hydrogel stiffness have been shown to affect the lineage choice of stem cells and cellular proliferation [108].…”

Section: Gradient Samplesmentioning

confidence: 99%

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Hydrogels in Tissue Engineering

2018

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Section: Gradient Samplesmentioning

confidence: 99%

Section: Gradient Samplesmentioning

confidence: 99%

Hydrogels in Tissue Engineering

2018

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“…Over the last decade, a series of 3D RP-based approaches have been developed in our group, the Center of Organ Manufacturing, Department of Mechanical Engineering, Tsinghua University ( Figure 2) [8][9][10][11] . Various complex vascular systems for different complex organ manufac- [40][41][42][43][44][45][46][47][48][49][50][51] . (B) Cell assembling based on a two syringe RP technique.Two different cell types in the gelatin-based hydrogels can be assembled simultaneously into a construct [52,53] .…”

Section: Introduction Of Several Bioprinting Techniques In Tsinghua Umentioning

confidence: 99%

Creation of a vascular system for organ manufacturing

Liu¹,

Wang²

2024

IJB

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Abstract:The creation of a vascular system is considered to be the main object for complex organ manufacturing. In this short review, we demonstrate two approaches to generate a branched vascular system which can be printed using rapid prototyping or bioprinting techniques. One approach is constructing mathematical tree models on the basis of human physiological characteristics and calculating the model using constrained constructive optimization to obtain three-dimensional (3D) geometrical structures. The rules of the branching of the vessel tree were extracted from the literature. Another approach is using computer-aided design models to build a multi-scale vascular network including arteries, veins, and capillaries. A 3D vascular template with both synthetic scaffold polymer and cell/hydrogel was created in our group, using a double-nozzle, low-temperature deposition technique. Each of the approaches holds promise in producing a vascular system.

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“…Improved cell differentiation and self-organization Drug discovery [75] 3D plotting Alginate Gellan gum Improved swelling ratio and stiffness General [76] 3D plotting PCL PEG, HAc Improved storage modulus General [77] 3D plotting PEG P(HPMAm-lactate)…”

mentioning

confidence: 99%

“…Xu et al fabricated a composite extracellular matrix by printing alginate/gelatin hydrogels with incorporating fibrin which is a natural scaffold material [75] . Fibrin acted as an important component to regulate self-organization and differentiation of adipose derived stromal cell.…”

mentioning

confidence: 99%

3D printing of hydrogel composite systems: Recent advances in technology for tissue engineering

Jang¹,

Jung²,

Pan³

et al. 2024

IJB

193

117

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Three-dimensional (3D) printing of hydrogels is now an attractive area of research due to its capability to fabricate intricate, complex and highly customizable scaffold structures that can support cell adhesion and promote cell infiltration for tissue engineering. However, pure hydrogels alone lack the necessary mechanical stability and are too easily degraded to be used as printing ink. To overcome this problem, significant progress has been made in the 3D printing of hydrogel composites with improved mechanical performance and biofunctionality. Herein, we provide a brief overview of existing hydrogel composite 3D printing techniques including laser based-3D printing, nozzle based-3D printing, and inkjet printer based-3D printing systems. Based on the type of additives, we will discuss four main hydrogel composite systems in this review: polymer-or hydrogel-hydrogel composites, particle-reinforced hydrogel composites, fiber-reinforced hydrogel composites, and anisotropic filler-reinforced hydrogel composites. Additionally, several emerging potential applications of hydrogel composites in the field of tissue engineering and their accompanying challenges are discussed in parallel.

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An cell-assembly derived physiological 3D model of the metabolic syndrome, based on adipose-derived stromal cells and a gelatin/alginate/fibrinogen matrix

Cited by 138 publications

References 51 publications

Hydrogels in Tissue Engineering

Hydrogels in Tissue Engineering

Creation of a vascular system for organ manufacturing

3D printing of hydrogel composite systems: Recent advances in technology for tissue engineering

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