An ecological study of diarrhoeagenic &lt;i&gt;E.coli&lt;/i&gt; associated with indiscriminate waste dumps and under five diarrhoea in six informal settlements in Durban, eThekwini Municipality, South Africa

Ramlal, Preshod Sewnand; Kistnasamy, Emilie Joy; Olaniran, Ademola O.

doi:10.1504/ijewm.2017.090056

Cited by 3 publications

(4 citation statements)

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“…Study has reported that infections due to EAEC could be resulted from intestinal inflammation, leading to childhood malnutrition and growth impairment (Tanih et al, 2019). The proportion of non-hybrid EAEC (10.1%) identified in this present study was lower as compared to 21.3% and 64.1% reported by Ramlal et al (2017) and Moyo et al (2007) respectively. However, our finding of was higher than the 2% reported by Aijuka et al (2018).…”

Section: Detection Of Diarrhoeagenic E Coli Pathotypescontrasting

confidence: 66%

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Virulence and phylogenetic analysis of enteric pathogenic Escherichia coli isolated from children with diarrhoea in South Africa

Alfinete

Bolukaoto

Heine

et al. 2022

International Journal of Infectious Diseases

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Section: Detection Of Diarrhoeagenic E Coli Pathotypescontrasting

confidence: 66%

“…The difference in EAEC prevalence reported in these studies might be due to the types of samples analysed and geographic region. When EAEC as well as hybrids that had the EAEC pathotype were considered, the proportion increased to 41.8%, thus making it higher than the one reported by Ramlal et al (2017).…”

Section: Detection Of Diarrhoeagenic E Coli Pathotypesmentioning

confidence: 79%

Virulence and phylogenetic analysis of enteric pathogenic Escherichia coli isolated from children with diarrhoea in South Africa

Alfinete

Bolukaoto

Heine

et al. 2022

International Journal of Infectious Diseases

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“…The detection of the stx gene in river water is of concern because the colonisation of the human large intestine with EHEC stx even in low proportions can result in potentially fatal complications, such as haemolytic uremic syndrome (HUS) [ 20 ]. Previous studies conducted in South Africa have reported EHEC rates of 15.0% [ 40 ], 15.08% [ 49 ] and 8.3% [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

Occurrence of Hybrid Diarrhoeagenic Escherichia coli Associated with Multidrug Resistance in Environmental Water, Johannesburg, South Africa

et al. 2021

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This study was undertaken to determine the virulence and antibiotic resistance profiles of diarrhoeagenic Escherichia coli (DEC) in environmental waters of Johannesburg, South Africa. Samples were collected and cultured on selective media. An 11-plex PCR assay was used to differentiate five DEC, namely: enteroaggregative (EAEC), enterohaemorrhagic (EHEC), enteroinvasive (EIEC), enteropathogenic (EPEC) and enterotoxigenic (ETEC). The antibiotic resistance profile of isolates was determined using the VITEK®-2 automated system. The virulence profiles of 170 E. coli tested showed that 40% (68/170) were commensals and 60% (102/170) were pathogenic. EPEC had a prevalence of 19.2% (32/170), followed by ETEC 11.4% (19/170), EAEC 6% (10/170) and EHEC 3% (5/170). Hybrid DEC carrying a combination of simultaneously two and three pathogenic types was detected in twenty-eight and nine isolates, respectively. The antibiotic susceptibility testing showed isolates with multidrug resistance, including cefuroxime (100%), ceftazidime (86%), cefotaxime (81%) and cefepime (79%). This study highlighted the widespread occurrence of DEC and antibiotic resistance strains in the aquatic ecosystem of Johannesburg. The presence of hybrid pathotypes detected in this study is alarming and might lead to more severe diseases. There is a necessity to enhance surveillance in reducing the propagation of pathogenic and antibiotic-resistant strains in this area.

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“…The identification of EAEC is mainly by PCR, HEp-2cell culture, and DNA probes (Oundo et al, 2009). Serological tests are used to differentiate diarrheagenic E. coli from other E. coli but direct identification include the use of DNA probes and PCR techniques (Qadri et al, 2005;Ramlal et al, 2017). There is inadequate data on the role money and cellphones play in the transmission of foodborne pathogens in Kenya.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization and Antibiotic Profile of Diarrhogenic E.Coli Isolated from Money and Cellphones of Food Handlers in Nairobi, Kenya

2018

IST Journal

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Escherichia coli is nonpathogenic facultative flora of the human intestine. However, some strains have transformed to become pathogenic hence diarrheagenic E. coli. There is inadequate data on the role money and cellphones play in the transmission of E. coli and their antibiotic profiles in Kenya. This study aimed at characterizing diarrhogenic E. coli and antibiotics profiles determination. The study participants were enrolled from selected food handling establishments in Kenya. Swabs from money and cell-phones were collected and cultured in appropriate media for the isolation, identification of E. coli and antimicrobial susceptibility testing. The E. coli pathogens showed some degree of resistant against ampicillin, Sulphamenthoxazole, streptomycin and tetracyclin. Resistance may be associated with decreased potency that can be due to drug degradation/adulteration, or presence of a lower concentration of active ingredients. Six strains of E. coli had a combination of genes coding for; EPEC, EHEC and EIEC. Pathogenic E. coli are a major causes of diarrheal diseases hence significant in this study since they were isolated from food handlers. Money and cell phones are possible vectors of pathogenic E. coli which are antibiotic resistant and can act as sources of outbreaks if proper hand hygiene is not observed.

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An ecological study of diarrhoeagenic <i>E.coli</i> associated with indiscriminate waste dumps and under five diarrhoea in six informal settlements in Durban, eThekwini Municipality, South Africa

Cited by 3 publications

References 0 publications

Virulence and phylogenetic analysis of enteric pathogenic Escherichia coli isolated from children with diarrhoea in South Africa

Virulence and phylogenetic analysis of enteric pathogenic Escherichia coli isolated from children with diarrhoea in South Africa

Occurrence of Hybrid Diarrhoeagenic Escherichia coli Associated with Multidrug Resistance in Environmental Water, Johannesburg, South Africa

Molecular Characterization and Antibiotic Profile of Diarrhogenic E.Coli Isolated from Money and Cellphones of Food Handlers in Nairobi, Kenya

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