An edible kanamycin sulfate cross-linked cellulose active against multiple pathogenic bacteria

Gu, Jun; Zhang, Shuaifeng; Xia, Xuefeng; Zhang, Xuehan; Fan, Baochao; Zhou, Junming; Zhu, Hua; Wang, Wei; Qi, Xianghui; Li, Li; Li, Bin

doi:10.1016/j.ijbiomac.2021.11.085

Cited by 8 publications

(8 citation statements)

References 33 publications

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“…In addition, there was an obvious enhancement of the peak at 286.5 eV, contributing more than 32%. This obvious enhancement could be attributed to the coordinated contribution of C–O and C–N bonds. , These results further confirmed the correctness of our presented reactions (Figure A). HTCC cross-linked together via the Schiff base reaction and successfully entangled with AuNPs forming the HTCC/amylose/AuNPs …”

Section: Resultssupporting

confidence: 91%

“…Deconvolution of high-resolution C 1s core-level spectra of the resulting HTCC/amylose/AuNPs was used to obtain quantitative results for the functional group . However, it is not easy to determine the nature of the incorporated groups as the contribution from C–O and C–N groups can be overlapped. , Typical deconvoluted-C 1s core-level spectra of the amylose/AuNPs and HTCC/amylose/AuNPs are shown in Figure C,D, and the contribution of elemental carbon from different chemical functionalities after deconvolution are listed in Table . As shown in Figure C, C 1s core-level spectra of the amylose/AuNPs featured two peaks.…”

Section: Resultsmentioning

confidence: 99%

“…In addition to stable and large protein loading capacity, a suitable protein vaccine delivery carrier would have the lower cytotoxicity and the higher cellular uptake ability. , The cytotoxicity of HTCC/amylose/AuNPs and amylose/AuNPs with different concentrations on intestinal porcine epithelial cell line-J 2 (IPEC-J2) and mouse Leukemia cells of monocyte macrophage (Raw 264.7) were determined by CCK-8 (Figure A,B). Compared with the untreated cells, amylose/AuNPs did not produce the cytotoxicity after co-incubation with IPEC-J2 or Raw 264.7 cells suggesting low toxicity.…”

Section: Resultsmentioning

confidence: 99%

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Positively Charged-Amylose-Entangled Au-Nanoparticles Acting as Protein Carriers and Potential Adjuvants to SARS-CoV-2 Subunit Vaccines

Fan

et al. 2023

ACS Appl. Mater. Interfaces

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The COVID-19 pandemic continues to spread worldwide. To protect and control the spread of SARS-CoV-2, varieties of subunit vaccines based on spike (S) proteins have been approved for human applications. Here, we report a new subunit vaccine design strategy that functions as both an antigen carrier and an adjuvant in immunization to elicit high-level immune responses. The complex of 2-hydroxypropyl-trimethylammonium chloride chitosan and amylose entangles Au nanoparticles (HTCC/amylose/AuNPs) forming 40 nm nanocarriers with a positive charge. The obtained positively charged nanoparticles reveal many merits, including the larger S protein loading capacity in PBS buffer, higher cellular uptake ability, and lower cell cytotoxicity, supporting their potential as safe vaccine nanocarriers. Two functionalized nanoparticle subunit vaccines are prepared via loading full-length S proteins derived from SARS-CoV-2 variants. In mice, both prepared vaccines elicit high specific IgG antibodies, neutralize antibodies, and immunoglobulin IgG1 and IgG2a. The prepared vaccines also elicit robust T- and B-cell immune responses and increase CD19+ B cells, CD11C+ dendritic cells, and CD11B+ macrophages at the alveoli and bronchi of the immunized mice. Furthermore, the results of skin safety tests and histological observation of organs indicated in vivo safety of HTCC/amylose/AuNP-based vaccines. Summarily, our prepared HTCC/amylose/AuNP have significant potential as general vaccine carriers for the delivery of different antigens with potent immune stimulation.

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Positively Charged-Amylose-Entangled Au-Nanoparticles Acting as Protein Carriers and Potential Adjuvants to SARS-CoV-2 Subunit Vaccines

Fan

et al. 2023

ACS Appl. Mater. Interfaces

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“…is shown in Figure 6C. A total of antibacterial substances (Hydroxyphenyl lactic acid, Phenyl lactic acid, Butane dioic acid, 2,3-Butanediol, Natamycin, Caffeic acid, Glucoside, Gallic acid, Neomycin, Lymecycline, Kanamycin, Ganoderic acid B, Ganoderic acid S, 1,4-Dihydroxyanthraquinone; 23.01%; Zheng et al, 2019Zheng et al, , 2020Wang et al, 2020Wang et al, , 2021aAbdul-Rahim et al, 2021;Sorgi et al, 2021;Gu et al, 2022;Selvaraj et al, 2022;Wu et al, 2023), corrosion inhibitors (4.31%), amino acids (37.99%), polysaccharides (9.16%), nucleosides (2.21%), phospholipids (3.62%), alkaloids (7.41%), organic acids (7.05%), and other substances (5.24%). The highest relative percentages and the largest variety of substances were amino acids, followed by antibacterial substances.…”

Section: Analysis Of Metabolite Compositionmentioning

confidence: 99%

Effect of Alcaligenes sp. on corrosion behavior of X65 steel in simulated offshore oilfield-produced water

Shi

Wang

2023

Front. Microbiol.

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In this paper, the effect of Alcaligenes sp. on the corrosion process of X65 steel was investigated by using non-targeted metabolomics techniques for comprehensive characterization of metabolites, combined with surface analysis techniques and electrochemical testing. The results showed that the organic acids produced by Alcaligenes sp. accelerated the corrosion process of X65 steel in the early stage, and the presence of Alcaligenes sp. promoted the deposition of stable corrosion products and minerals in the middle and late stages. In addition, proteoglycans and corrosion inhibiting substances were enriched on the metal surface, which enhanced the stability of the film. The combined effect of multiple factors makes the mixed film of biofilm and corrosion products more dense and complete, which effectively inhibits the corrosion of X65 steel.

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“…The cytotoxicity of HTCC/starch particles was investigated by testing the cell viability of Vero and LLC-PK1 cells as model cells. 25 Before detection, Vero cells (ATCC No. CCL-81) were cultured in DMEM supplemented with antibiotics (1% (w/v) penicillin and 1% (w/v) streptomycin) and 5% (v/v) heat-inactivated FBS.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

Low-Cytotoxicity, Broad-Spectrum Corn Starch-Based Antibacterial Particles that Inhibit Multidrug-Resistant Bacteria

Deng,

Gu,

Zhang

et al. 2023

ACS Appl. Bio Mater.

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Antimicrobial resistance is a serious problem in biomedical applications that seriously increases the risk of medical failure. Therefore, developing highly efficient antibacterial agents that inhibit the growth of multidrug-resistant bacteria is a long-standing research goal. In this report, a low-cytotoxicity and highly efficient alternative to antibiotics was designed and prepared using edible corn starch as the scaffold and 2-hydroxypropyl-trimethylammonium chloride chitosan (HTCC) as the antimicrobial agent. The HTCC/ starch particles were found to have a positively charged surface over a wide pH range and to possess broad-spectrum and highly efficient antimicrobial properties. These particles inhibited the growth of standard Gram-positive and Gram-negative bacteria from the China Pharmacopoeia and a clinical multidrug-resistant bacterial strain. Moreover, after treating the HTCC/starch particles with simulated gastric fluid (SGF, pH 1.2) for 4 h, the growth of clinical multidrug-resistant Escherichia coli (NT 2036) was inhibited effectively, indicating that these particles tolerate a gastric acid environment. Although the mass of SGF-treated HTCC/starch particles required to achieve similar antibacterial activity was ∼20-fold that of chloramphenicol or ampicillin, antibiotic-containing products require considerable amounts of pharmaceutical excipients to prepare. Therefore, the HTCC/starch particles described herein are potentially cost-effective alternatives to antibiotics that resolve the antimicrobial resistance issue, especially for inhibiting the growth of pathogenic intestinal bacteria.

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An edible kanamycin sulfate cross-linked cellulose active against multiple pathogenic bacteria

Cited by 8 publications

References 33 publications

Positively Charged-Amylose-Entangled Au-Nanoparticles Acting as Protein Carriers and Potential Adjuvants to SARS-CoV-2 Subunit Vaccines

Positively Charged-Amylose-Entangled Au-Nanoparticles Acting as Protein Carriers and Potential Adjuvants to SARS-CoV-2 Subunit Vaccines

Effect of Alcaligenes sp. on corrosion behavior of X65 steel in simulated offshore oilfield-produced water

Low-Cytotoxicity, Broad-Spectrum Corn Starch-Based Antibacterial Particles that Inhibit Multidrug-Resistant Bacteria

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