An Effective Immunization of Experimental Monkeys Against a Human Malaria Parasite,
            <i>Plasmodium falciparum</i>

Siddiqui, Wasim A.

doi:10.1126/science.406671

Cited by 102 publications

(44 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results are similar to those obtained using whole merozoites and/or schizonts as immunogens (11,12,13,14). The protective effect induced by immunization with the 41-kD polypeptide is probably not dependent on strain specific determinant, on the basis of several observations.…”

Section: Discussionsupporting

confidence: 74%

Immunization with a Plasmodium falciparum merozoite surface antigen induces a partial immunity in monkeys.

Perrin¹,

Merkli²,

Gabra³

et al. 1985

J. Clin. Invest.

View full text Add to dashboard Cite

Saimiri monkeys immunized with a Plasmodium falciparum merozoite polypeptide of 41 kD mol wt are resistant to a blood challenge infection that induces a fulminant infection in control monkeys. The sera of the immunized monkeys reacted, as shown by the indirect immunofluorescence technique, with the apical part of the merozoites from five isolates or clones of P. falciparum. Whether the immunogen was dissolved in nonionic detergent (NP-40) or in sodium dodecyl sulfate (SDS) had a marked influence on the level of protection in immunized monkeys. Thus, monkeys immunized with the antigen solubilized in a nonionic detergent developed much lower parasitemia than monkeys immunized with denatured antigen (antigen eluted from SDS polyacrylamide gel electrophoresis).

show abstract

Section: Discussionsupporting

confidence: 74%

Immunization with a Plasmodium falciparum merozoite surface antigen induces a partial immunity in monkeys.

Perrin¹,

Merkli²,

Gabra³

et al. 1985

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

“…With P. falciparum, Voller and Richards observed a significant delay in the prepatent period following immunization of Aotus monkeys with formalin-treated schizonts of P. falciparum in adjuvant, but the induced immunity was not sufficient to protect the monkeys against death (312). Partial protection against homologous as well as heterologous P. falciparum challenge was induced in Aotus monkeys by immunization with P. falciparum merozoites in adjuvant (279,280). Immunization with mature schizont stages of P. falciparum conferred strong protection against homologous challenge in Saimiri monkeys (106), and Saimiri monkeys develop significant, long-lasting protection (up to 7 months) against challenge with homologous as well as (61,248), against P. lophurae in ducks and chickens (253), and against P. yoelii nigeriensis in mice (314).…”

Section: Empirical Observations Of Asexual Erythrocytic-stage Immunitymentioning

confidence: 99%

Acquired Immunity to Malaria

2009

View full text Add to dashboard Cite

SUMMARY Naturally acquired immunity to falciparum malaria protects millions of people routinely exposed to Plasmodium falciparum infection from severe disease and death. There is no clear concept about how this protection works. There is no general agreement about the rate of onset of acquired immunity or what constitutes the key determinants of protection; much less is there a consensus regarding the mechanism(s) of protection. This review summarizes what is understood about naturally acquired and experimentally induced immunity against malaria with the help of evolving insights provided by biotechnology and places these insights in the context of historical, clinical, and epidemiological observations. We advocate that naturally acquired immunity should be appreciated as being virtually 100% effective against severe disease and death among heavily exposed adults. Even the immunity that occurs in exposed infants may exceed 90% effectiveness. The induction of an adult-like immune status among high-risk infants in sub-Saharan Africa would greatly diminish disease and death caused by P. falciparum. The mechanism of naturally acquired immunity that occurs among adults living in areas of hyper- to holoendemicity should be understood with a view toward duplicating such protection in infants and young children in areas of endemicity.

show abstract

“…The ability of some species of new world monkeys to support the erythrocytic stage of malarial infection has allowed vaccination studies to be undertaken in these primates and early experiments showed that vaccination with merozoites or schizonts from Plasmodium falciparum protected against a lethal bloodstage infection (Mitchell et al 1977;Siddiqui, 1977). Many merozoite and schizont antigens have since been identified and their genes cloned and sequenced.…”

Section: Introductionmentioning

confidence: 99%

A rhoptry antigen ofPlasmodium falciparumis protective inSaimirimonkeys

Ridley¹,

Takács

Etlinger

et al. 1990

Parasitology

View full text Add to dashboard Cite

SUMMARYA non-polymorphic antigen associated with the rhoptry organelles of Plasmodium falciparum has been purified by immunoaffinity chromatography. The antigen, RAP-1 (rhoptry associated protein-1), which is defined by monoclonal antibodies which inhibit parasite growth in vitro, is a multi-component antigen consisting of four major proteins of 80, 65, 42 and 40 kDa and two minor proteins of 77 and 70 kDa. These proteins were electro-eluted from preparative sodium dodecyl sulphate polyacrylamide gels and protected Saimiri sciureus monkeys from a lethal blood-stage infection of P. falciparum malaria. Sera from the protected animals recognized only proteins of the RAP-1 antigen when used to probe a Western blot of total parasite protein extract, confirming that RAP-1 is responsible for eliciting the protective immune response.

show abstract

An Effective Immunization of Experimental Monkeys Against a Human Malaria Parasite, Plasmodium falciparum

Cited by 102 publications

References 21 publications

Immunization with a Plasmodium falciparum merozoite surface antigen induces a partial immunity in monkeys.

Immunization with a Plasmodium falciparum merozoite surface antigen induces a partial immunity in monkeys.

Acquired Immunity to Malaria

A rhoptry antigen ofPlasmodium falciparumis protective inSaimirimonkeys

Contact Info

Product

Resources

About