An effective method for saliva stabilization and magnetic nanoparticles based DNA extraction for genomic applications

Bhati, Aniruddha; Varghese, Anu; Rajan, Gayathri; Sridhar, Vandana; Mohan, Y. Rama; Pradeep, Swetha; Babu, S. N. Vinaya; Kaikkolante, Nimisha; Sarma, Minu; Arun, Sreepriya; Sekar, Arun Prasath; Iype, Tessy; Santhosh, Sam; Ramchand, C.N.

doi:10.1016/j.ab.2021.114182

Cited by 14 publications

(10 citation statements)

References 24 publications

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“…When a magnetic field is applied, MNPs aggregate toward the magnet, and upon removing the magnetic field, MNPs disperse in solution. Consequently, MNPs have found extensive use in the separation and purification of various biological samples, including cells ( Bhati et al, 2021 ), proteins ( Sharma et al, 2022 ), and nucleic acids ( Nguyen et al, 2022 ; Huang et al, 2023 ). MNPs are particularly effective in recognizing whole-cell bacteria in large samples without the need for enrichment processes, concentration, identification, or quantification of bacterial samples.…”

Section: The Impact Of Magnetic Nanoparticles On Biological Detectionmentioning

confidence: 99%

Advancements in magnetic nanoparticle-based biosensors for point-of-care testing

Wang,

Jin,

Hang-Mei Leung

et al. 2024

Front. Bioeng. Biotechnol.

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The significance of point-of-care testing (POCT) in early clinical diagnosis and personalized patient care is increasingly recognized as a crucial tool in reducing disease outbreaks and improving patient survival rates. Within the realm of POCT, biosensors utilizing magnetic nanoparticles (MNPs) have emerged as a subject of substantial interest. This review aims to provide a comprehensive evaluation of the current landscape of POCT, emphasizing its growing significance within clinical practice. Subsequently, the current status of the combination of MNPs in the Biological detection has been presented. Furthermore, it delves into the specific domain of MNP-based biosensors, assessing their potential impact on POCT. By combining existing research and spotlighting pivotal discoveries, this review enhances our comprehension of the advancements and promising prospects offered by MNP-based biosensors in the context of POCT. It seeks to facilitate informed decision-making among healthcare professionals and researchers while also promoting further exploration in this promising field of study.

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Section: The Impact Of Magnetic Nanoparticles On Biological Detectionmentioning

confidence: 99%

Advancements in magnetic nanoparticle-based biosensors for point-of-care testing

Wang,

Jin,

Hang-Mei Leung

et al. 2024

Front. Bioeng. Biotechnol.

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show abstract

“…Poor‐quality samples can lead to inaccurate or misleading results, which can be problematic in both clinical and research settings. To minimize the risk of contamination and poor sample quality, it is crucial to follow strict collection and handling protocols, including using sterile collection devices, avoiding food and drink before collection, and proper storage of samples 135 Interference from oral care products: Saliva analysis is also subject to interference from oral care products.…”

Section: Challenges and Prospectsmentioning

confidence: 99%

Salivary analysis: An emerging paradigm for non‐invasive healthcare diagnosis and monitoring

Liao

Chen

2023

Interdisciplinary Medicine

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As an emerging non-invasive and easy-to-use toolkit, salivary analysis has garnered significant attention recently. By leveraging the latest discoveries in salivary biomarkers, salivary analysis has the potential to revolutionize the field of healthcare by providing valuable information for monitoring an individual's health status, diagnosing disease progression, and enabling personalized treatment. However, the transitional development of breakthrough salivary analysis remains an intriguing challenge. Only a few innovations have progressed to new transformative healthcare diagnosis and monitoring tools. In this review, we attempt to illustrate a bird's eye view of the emerging field of salivary analysis by exploring an assorted selection of seminar progress that can potentially shape the developing trajectory of the field of salivary analysis. The fundamental nature of saliva and the versatile robust uses of salivary analysis as a novel non-invasive method in various healthcare diagnostic and monitoring fields will be thoroughly discussed. To inspire next-stage development in this thriving and fruitful field, we also crystalize key findings, pinpoint challenges that remain to be addressed and present an outlook on the future transitional development of salivary analysis, invigorating future research of groundbreaking non-invasive healthcare diagnosis and monitoring strategies, and beyond.

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“…The inherent properties of MNPs, such as high surface-to-volume ratio, magnetically controlled particle aggregation and dispersion( Ma et al, 2019 ) and their ability to bind to a large number of different biomolecules (DNA, RNA, enzymes), offer a promising platform for the isolation of DNA and RNA from complex samples as well as enriching nucleic acids that facilitates their detection( Farinha et al, 2021 ). In recent years, MNPs have been applied for nucleic acid separation and purification, which could bind nucleic acid by the free chemical groups modified on the surface( Bhati et al, 2021 ; Oberacker et al, 2019 ; Ota et al, 2006 ). Using magnetic separation methods, nucleic acids can be directly isolated from crude biological samples without any restrictions with respect to the sample volumes.…”

Section: Nucleic Acid Extraction Based On Magnetic Nanomaterialsmentioning

confidence: 99%

“…Bhati et al developed an improved method for DNA extraction from human saliva using bare MNPs, and they found that the yield and purity of DNA was higher compared to other methods. In addition, this method requires no centrifugation step while it is mandatory for the spin column-based techniques ( Bhati et al, 2021 ). MNPs have been used in RT-qPCR diagnosis for the extraction of viral RNA from SARS-CoV-2.…”

Section: Nucleic Acid Extraction Based On Magnetic Nanomaterialsmentioning

confidence: 99%

Nanotechnology-based diagnostic methods for coronavirus: From nucleic acid extraction to amplification

Huang

Qiao

et al. 2023

Biosensors and Bioelectronics: X

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An effective method for saliva stabilization and magnetic nanoparticles based DNA extraction for genomic applications

Cited by 14 publications

References 24 publications

Advancements in magnetic nanoparticle-based biosensors for point-of-care testing

Advancements in magnetic nanoparticle-based biosensors for point-of-care testing

Salivary analysis: An emerging paradigm for non‐invasive healthcare diagnosis and monitoring

Nanotechnology-based diagnostic methods for coronavirus: From nucleic acid extraction to amplification

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