An effective pathway for the removal of microcystin LR via anoxic biodegradation in lake sediments

Chen, Xiaoguo; Xia, Yang; Yang, Lili; Xiao, Baihua; Wu, Xingqiang; Wang, Jutao; Wan, Hongguo

doi:10.1016/j.watres.2009.11.025

Cited by 98 publications

(69 citation statements)

References 31 publications

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“…Nevertheless, sediment, which often serves as one of the most important sources and sinks of environmental pollutants, may also play an important role in the fate of MCs. In recent years, numerous studies have reported that MCs can be strongly adsorbed [10][11][12] and favor biotic degradation [10,13,14] in sediments. However, in these studies, the sorption and biodegradation were calculated based on variations in the MCs concentration in the aqueous phase, the bioavailability, toxicity, and migratory activity of MCs in the sediment phase are still unclear, especially the level of bound residues resulting from chemical interactions.…”

Section: Introductionmentioning

confidence: 99%

Optimal strategies for determination of free/extractable and total microcystins in lake sediment

Wang

Xiao

et al. 2012

Analytica Chimica Acta

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Section: Introductionmentioning

confidence: 99%

Optimal strategies for determination of free/extractable and total microcystins in lake sediment

Wang

Xiao

et al. 2012

Analytica Chimica Acta

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“…Y2 was reported to have the highest degradation rate of 5.4 mg$L -1 per day for MC-LR [8,22,24]. On the other hand, Holst et al [27] and Chen et al [28] investigated the potential for anoxic biodegradation of microcystin and the effects of environmental factors on the process through a series of wellcontrolled microcosm experiments.…”

Section: Introductionmentioning

confidence: 99%

Microcystin-LR biodegradation by Sphingopyxis sp. USTB-05

Xiao

Yan

Wang

et al. 2010

Front. Environ. Sci. Eng. China

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A promising bacterial strain for biodegrading microcystin-LR (MC-LR) as the sole carbon and nitrogen source was successfully isolated from Lake Dianchi, China. The strain was identified as Sphingopyxis sp. USTB-05, which was the first isolated MCs-biodegrading Sphingopyxis sp. in China. The average biodegradation rate of MC-LR by Sphingopyxis sp. USTB-05 was 28.8 mg$L -1 per day, which was apparently higher than those of other bacteria reported so far. The optimal temperature and pH for both strain USTB-05 growth and MC-LR biodegradation were 30°C and 7.0, respectively. The release of MC-LR from the cyanobacterial cells collected from Lake Guishui and the biodegradation of MC-LR by both strain and cell-free extract (CE) were investigated. The results indicated that MC-LR with the initial concentration of 4.0 mg$L -1 in water was biodegraded by Sphingopyxis sp. USTB-05 within 4 d, while MC-LR with the initial concentration of 28.8 mg$L -1 could be completely removed in 3 h by CE of Sphingopyxis sp. USTB-05 containing 350 mg$L -1 protein. During enzymatic biodegradation of MC-LR, two intermediate metabolites and a dead-end product were observed on an HPLC chromatogram. Moreover, the similar scanning profiles of MC-LR and its metabolic products indicate that the Adda side-chain of MC-LR was kept intact in all products.

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“…Some genera, such as Microcystis, can produce a wide range of toxins, including a family of hepatotoxins called microcystins (MCs) (Botes et al, 1984), which are the most frequently encountered cyanotoxins. More than 70 variants of MCs have been isolated and identified, among which MC-RR and MC-LR are considered the most common and dangerous variants (Chen et al, 2010). Since MCs are known to be tumor promoters to liver and colon (Zhao et al, 2011;Zhang et al, 2009), they pose a risk to human or animal health when surface waters are used as a drinking water source and/or for recreational purposes.…”

Section: Introductionmentioning

confidence: 99%

“…These values far exceed the provisional guideline value of 1.0 μg L −1 of MC-LR in drinking water recommended by World Health Organization. On the other hand, it is also well recognized that biodegradation is one of the elimination pathways to reduce its concentration in natural waters and sediment (Chen et al, , 2010. However, in severe cyano-HABs lakes, dramatic increased concentration of MCs after the cyano-HAB may exceed the endurance of indigenous MCs-degrading microorganisms (Ghernaout et al, 2010;Cai et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Enhanced and continued degradation of microcystins using microorganisms obtained through natural media

Hong

Pan

2014

Journal of Microbiological Methods

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Microorganisms isolated through artificial media are often unsustainable in biodegrading microcystins (MCs) in natural water. Here we studied alternative approaches to isolate MCs-degrading bacteria using natural media. In comparison to two species (MS-1 and MS-2) isolated from artificial media and the failure of bacterial colonies formation using water extracts of sediment (10%, w/v), five colony species (WC-1 to WC-5) appeared using concentrated water extracts of sediment that is 10-fold enhancement of nutrient level. In the simulated biodegradation test in Lake Taihu water with continuous supply of MCs, a lag phase of 6 days was required for MS-1 and M-2 to degrade 13% and 15% of the added MC-RR and MC-LR, respectively, whereas the lag phase was only 3 days with approximately 44% and 31% removal of the added MC-RR and MC-LR by WC-1 to WC-5. During the continuous supply experiment, degradation of MCs by MS-1 and MS-2 stopped after 3 days, while degradation of MCs by WC-1 to WC-5 lasted continuously throughout the 18 day test period with 2 to 6-fold enhancement of removal rate. 16S rRNA gene sequences and phylogenetic analysis indicated the potential to amplify species of MCsdegrading bacteria when natural media were used. The results suggested that the increased adaptability of bacteria obtained through concentrated natural media was responsible for the enhanced and continued biodegradation under simulated natural water conditions.

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An effective pathway for the removal of microcystin LR via anoxic biodegradation in lake sediments

Cited by 98 publications

References 31 publications

Optimal strategies for determination of free/extractable and total microcystins in lake sediment

Optimal strategies for determination of free/extractable and total microcystins in lake sediment

Microcystin-LR biodegradation by Sphingopyxis sp. USTB-05

Enhanced and continued degradation of microcystins using microorganisms obtained through natural media

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