An efficient and cost-effective method of generating postnatal (P2–5) mouse primary hippocampal neuronal cultures

Kaar, Aidan; Morley, Shane J.; Rae, Mark G.

doi:10.1016/j.jneumeth.2017.05.020

Cited by 4 publications

(1 citation statement)

References 28 publications

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“…Several postnatal culture protocols have been published to produce mouse primary neuronal culture from either very early stage after birth (Ahlemeyer and Baumgart-Vogt, 2005;Beaudoin et al, 2012;Kaar et al, 2017) or adult animals (Eide and McMurray, 2005;Peltier et al, 2010). Postnatal culture presents important advantages such as (i) reducing the number of experimental animals in agreement with the rule of the 3R, as only the pups required for the culture are sacrificed, (ii) genotyping of transgenic animals prior to the culture.…”

Section: Introductionmentioning

confidence: 99%

Procedures for Culturing and Genetically Manipulating Murine Hippocampal Postnatal Neurons

Moutin

Hemonnot-Girard

Seube

et al. 2020

Front. Synaptic Neurosci.

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Neuronal hippocampal cultures are simple and valuable models for studying neuronal function. While embryonic cultures are widely used for different applications, mouse postnatal cultures are still challenging, lack reproducibility and/or exhibit inappropriate neuronal activity. Yet, postnatal cultures have major advantages such as allowing genotyping of pups before culture and reducing the number of experimental animals. Herein we describe a simple and fast protocol for culturing and genetically manipulating hippocampal neurons from P0 to P3 mice. This protocol provides reproducible cultures exhibiting a consistent neuronal development, normal excitatory over inhibitory neuronal ratio and a physiological neuronal activity. We also describe simple and efficient procedures for genetic manipulation of neurons using transfection reagent or lentiviral particles. Overall, this method provides a detailed and validated protocol allowing to explore cellular mechanisms and neuronal activity in postnatal hippocampal neurons in culture.

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