An efficient and inexpensive method for functionalizing metallic biomaterials used in orthopedic applications

Hamdaoui, Soria; Lambert, Ambroise; Khireddine, Hafit; Agniel, Rémy; Cousture, Annelise; Coulon, Régis; Gallet, Olivier; Alfonsi, Séverine; Hindié, Mathilde

doi:10.1016/j.colcom.2020.100282

Cited by 11 publications

(5 citation statements)

References 63 publications

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“…PPy films had a thickness of 4.98 µm ± 0.15 µm and a low roughness of 0.27 µm ± 0.05 µm. In this study, the films obtained were thicker (4.9 µm vs. 2.2 µm) than those obtained by Hamdaoui S et al [37]. These results are consistent because the Py solution used was twice as concentrated as the one previously used in the lab.…”

Section: Electrodeposition Of Ppy Coatingssupporting

confidence: 89%

Fibronectin Conformations after Electrodeposition onto 316L Stainless Steel Substrates Enhanced Early-Stage Osteoblasts’ Adhesion but Affected Their Behavior

Alfonsi,

Karunathasan,

Mamodaly-Samdjee

et al. 2023

JFB

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The implantation of metallic orthopedic prostheses is increasingly common due to an aging population and accidents. There is a real societal need to implement new metal implants that combine durability, good mechanical properties, excellent biocompatibility, as well as affordable costs. Since the functionalization of low-cost 316L stainless steel substrates through the successive electrodeposition of a polypyrrole film (PPy) and a calcium phosphate deposit doped with silicon was previously carried out by our labs, we have also developed a bio-functional coating by electrodepositing or oxidating of fibronectin (Fn) coating. Fn is an extracellular matrix glycoprotein involved in cell adhesion and differentiation. Impacts of either electrodeposition or oxidation on the structure and functionality of Fn were first studied. Thus, electrodeposition is the technique that permits the highest deposition of fibronectin, compared to adsorption or oxidation. Furthermore, electrodeposition seems to strongly modify Fn conformation by the formation of intermingled long fibers, resulting in changes to the accessibility of the molecular probes tested (antibodies directed against Fn whole molecule and Fn cell-binding domain). Then, the effects of either electrodeposited Fn or oxidized Fn were validated by the resulting pre-osteoblast behavior. Electrodeposition reduced pre-osteoblasts’ ability to remodel Fn coating on supports because of a partial modification of Fn conformation, which reduced accessibility to the cell-binding domain. Electrodeposited Fn also diminished α5 integrin secretion and clustering along the plasma membrane. However, the N-terminal extremity of Fn was not modified by electrodeposition as demonstrated by Staphylococcus aureus attachment after 3 h of culture on a specific domain localized in this region. Moreover, the number of pre-osteoblasts remains stable after 3 h culture on either adsorbed, oxidized, or electrodeposited Fn deposits. In contrast, mitochondrial activity and cell proliferation were significantly higher on adsorbed Fn compared with electrodeposited Fn after 48 h culture. Hence, electro-deposited Fn seems more favorable to pre-osteoblast early-stage behavior than during a longer culture of 24 h and 48 h. The electrodeposition of matrix proteins could be improved to maintain their bio-activity and to develop this promising, fast technique to bio-functionalize metallic implants.

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Section: Electrodeposition Of Ppy Coatingssupporting

confidence: 89%

Fibronectin Conformations after Electrodeposition onto 316L Stainless Steel Substrates Enhanced Early-Stage Osteoblasts’ Adhesion but Affected Their Behavior

Alfonsi,

Karunathasan,

Mamodaly-Samdjee

et al. 2023

JFB

Self Cite

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“…It was reported that the NaSa is predominantly an anodic inhibitor [29]. In the case of molybdate was also used extensively as a corrosion inhibitor of iron and stainless steels over a wide-ranging temperature and pH range [11,13]. The anion positively affects the passivity breakdown and pitting initiation of 316L SS.…”

Section: Corrosion Inhibition Study Of 316l Ss In Presence Of Additivesmentioning

confidence: 99%

“…They are one of the oldest and most widely used anti-inflammatory drugs and also exhibit various recognized pharmacological roles such as anticancer, neuroprotective, antipyretic, skin antiaging, and antidiabetic effects [10]. It was reported that the presence of sodium salicylate in a polypyrrole coating improves the corrosion protection of 316L SS, promoting the formation of the passive layer on the surface of substrate [11,12]. Moreover, it has been informed a favorable in vivo vascular compatibility of a salicylate-based polymer coating employed as a drug-eluting for metallic stents [13].…”

Section: Introductionmentioning

confidence: 99%

Corrosion Protection of 316L SS by Cerium-Based Coatings: Effect of the Incorporation of Additives

C. Cortes,

P. Loperena,

I. Brugnoni

et al. 2023

New Advances in Steel Engineering

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Interest in cerium-based coatings has increased in recent years due to their low toxicity, biocompatibility, and improved corrosion protection performance. The formation of a coating from a solution containing cerium nitrate (Ce(NO3)3) has been investigated as a good surface modification strategy for the production of protective coatings on 316L SS. The effect of various additives in the treatment solution (sodium molybdate, sodium salicylate, and ascorbic acid) on the corrosion protection properties of the coatings was evaluated. The protection performance of the coated samples in a physiological simulated fluid (Ringer solution) was examined by electrochemical methods. The composition and morphology of all coatings were analyzed by scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDX).

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“…There is no need for surface preparation prior to EDM, and the process also converts the surface into a biocompatible oxide layer of significant thickness, increasing the biocompatibility of the substrate and creating a convenient environment for cell attachment and growth [93][94][95]. When compared to other surface modification procedures, the EDM process increases the micro-hardness, biocompatibility, wear, and corrosion resistance of implant surfaces by forming different carbides and intermetallic compounds on the coasted surface [96][97][98][99].…”

Section: Biomaterials Properties and Surface Modificationmentioning

confidence: 99%

Surface modification during hydroxyapatite powder mixed electric discharge machining of metallic biomaterials: a review

Bisaria

Patra

Mohanty

2022

Surface Engineering

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Surface modifications play a vital role in the performance of bio-implants. Powder mixed electric discharge machining (PM-EDM), a recently developed advanced machining method, can machine and coat the surface of conductive materials at the same time. Hydroxyapatite is a bio-ceramic with a bone-like composition and excellent biocompatibility. Several coating techniques are used to deposit a bio-ceramic layer on the implant surface; however, hydroxyapatite powder mixed-EDM (HAp-EDM) is an electro-thermal process that can be used for surface coating as well as machining of metallic biomaterials. In this review article, surface characteristics such as surface morphology/topography, micro-hardness, phase analysis, recast layer, elemental composition, corrosion/wear resistance, and biocompatibility of the coated surface of an implant after HAp-EDM have been meticulously reviewed. This review also looks at future research opportunities for the HAp-EDM process to meet the high standards required for biomedical materials and their applications in bio-implant manufacturing.

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An efficient and inexpensive method for functionalizing metallic biomaterials used in orthopedic applications

Cited by 11 publications

References 63 publications

Fibronectin Conformations after Electrodeposition onto 316L Stainless Steel Substrates Enhanced Early-Stage Osteoblasts’ Adhesion but Affected Their Behavior

Fibronectin Conformations after Electrodeposition onto 316L Stainless Steel Substrates Enhanced Early-Stage Osteoblasts’ Adhesion but Affected Their Behavior

Corrosion Protection of 316L SS by Cerium-Based Coatings: Effect of the Incorporation of Additives

Surface modification during hydroxyapatite powder mixed electric discharge machining of metallic biomaterials: a review

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