An efficient and rapid regeneration via multiple shoot induction from mature seed derived embryogenic and organogenic callus of Indian maize (<i>Zea mays</i>L.)

Pathi, Krishna Mohan; Tula, Suresh; Huda, Kazi Md. Kamrul; Srivastava, Vineet K.; Tuteja, Narendra

doi:10.4161/psb.25891

Cited by 21 publications

(19 citation statements)

References 32 publications

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“…In Bermuda grass [41] and maize [22], it has been reported that the inclusion of a low concentration of cytokinin in callus induction medium having 2,4-D promoted the induction of embryogenic callus. In our study also, the supplementation of Kinetin to callus induction medium having 2,4-D significantly increased the frequency of embryogenic callus formation,which is consistent with the results reported by Pathi et al [16] and Zhong et al [17].…”

Section: Plantlet Regenerationsupporting

confidence: 93%

“…Plant regeneration has also been reported from calli initiated from different explants, namely, anthers [2][3][4], glumes [5], immature inflorescence [6], immature tassels [7][8][9], leaf segments [10][11][12], protoplasts [13], seedling segments [14][15][16], shoot tips [17][18][19], shoot apical meristem [20], mature embryos [15,21,22], and immature embryos [23][24][25][26][27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

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Coleoptilar node - A season-independent explant source for in vitro culture in maize (Zea mays L.)

2018

J App Biol Biotech

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In vitro plant regeneration is preliminary requisite in the process of obtaining transgenic plants. Somatic embryogenesis is the most frequently used method of in vitro plant regeneration in maize. In this study, a reliable and reproducible maize regeneration method has been standardized using mature embryos. Inspite, the immature embryos are most suitable explants for somatic embryogenesis, obtaining these explants continuously is a laborious and tedious process, especially in summer. Hence, alternate explants of maize for in vitro regeneration have been chosen from different parts of embryonic axis. This study provides an evaluation of inbred lines and effect of hormonal combinations on total and embryogenic callus induction, their growth rate, and plantlet regeneration. Coleoptilar nodal explants from four inbred lines were cultured on different concentrations and combinations of 2,4-dichloro phenoxyacetic acid (2,4-D) along with kinetin (Kn). Explants grown on Murashige and Skoog medium supplemented with 2,4-D and Kn at a concentration of 5 and 1 mg/L, respectively, were found to be the best one for yielding a high frequency of embryogenic callus. Inbred line, NM81A was found to be the best one for in vitro culture among the four lines used. Combination of 6-benzylaminopurine and Kn at a concentration of 1 mg/L each promoted the highest frequency of shoot induction.

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Section: Plantlet Regenerationsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Coleoptilar node - A season-independent explant source for in vitro culture in maize (Zea mays L.)

2018

J App Biol Biotech

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“…Pathi et al in 2013 reported that the maximum frequency of embryogenic callus formation (90%) was obtained on MS medium supplemented with 2 mg/l 2,4-D and 1 mg/l BAP in the dark conditions, while the compact granular organogenic callus formation (85% frequency) was obtained on MS medium supplemented with 2.5 mg/l 2,4-D and 1.5 mg/l BAP at light conditions. On the other hand, Gorji et al, 2011 using N6 medium supplemented with 2 mg/L Dicamba induced the highest frequency of organogenic callus.…”

Section: Resultsmentioning

confidence: 99%

In Vitro Selection of Local Maize (Zea mays) on NaCl Stress and Its Genetic Characterization using RAPD

et al. 2016

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Maize (Zea mays) is one of graminae plants that widely spread for many purposes wheter in food industry, feed, or bioenergy. Those high utilization required an increment in production, but unfortunately the the demands not in accordance with the volume of production since conversion of agricultural area increase lately. Indonesia has many of shoreline that recognized as marginal land where the salinity is high as well. This research try to obtain tolerant variant from two local cultivars that planted in Madura Island. Manding and Talango varieties were used as an explant for callus induction stage in MS supplemented with 2,4 D. The result showed that 4 ppm of 2,4 D were the best concentration to induce the callus in both varieties. The induced callus were exposed to medium MS that contained NaCl (0, 2500, 5000, and 7500 ppm). In 7500 ppm of NaCl, Manding variety has 100% of surviving callus, while Talango variety only 66,7%. Furthermore, Manding variety showed a better performance in callus weight improvement with 170 mg, while Talango gave no improvement in callus weight. The result of RAPD analysis indicated that the genome characteristic was different between initial callus and surviving callus. Only five primers were presence polymorphism i.e OPA 13, OPB 07, OPC 02, OPK 20, and OPU 19 from ten in total primers. Manding elected as high tolerance variety in Salinity stress, thus it proposed to be developed furthermore.

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“…Internal factors included less development of wax/cuticle layer, slight number of palisade cells, less development of vascular tissue from the roots to the shoots and minimum lignification of stem, while external factors were growth medium, humidity, light, temperature and pathogens (Gunawan 1992). The other factors affecting acclimatization were medium treatment, pot type and humidity (Pathi et al 2013). A small pot composed of a mixture of peat moss, vermiculite and sand in the ratio of 1:1:1, and the increasing humidity by covering with polyethylene bags with small hole resulted in good plant regeneration.…”

Section: Acclimatization Of Transformants and Molecular Analysismentioning

confidence: 99%

Agrobacterium tumefaciens-MEDIATED IN-PLANTA TRANSFORMATION OF INDONESIAN MAIZE USING pIG121Hm-Cs PLASMID CONTAINING nptII AND hpt GENES

Listanto¹,

Riyanti²,

Sustiprijatno³

2017

Indones. J. Agric. Sci.

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Maize (Zea mays L.) productivity in Indonesia is challenged to be increased using genetic engineering. Recent advances in Agrobacterium tumefaciens-mediated in-planta transformation makes it possible to transform maize with low cost, and simple method. This study aimed to confirm pIG121Hm-Cs plasmid in A. tumefaciens, and to estimate the efficiency level of A. tumefaciens-mediated in-planta transformation of Indonesian maize by using pIG121Hm-Cs plasmid containing nptII and hpt genes. A series of studies were conducted including confirmation of gene construct of pIG121Hm-Cs plasmid in A. tumefaciens, transformation of four maize lines through A. tumefaciens-mediated in-planta technique, acclimatization of transformant plants and molecular analysis of selected plants using polymerase chain reaction (PCR). The pIG121Hm-Cs plasmid was confirmed via PCR analysis using specific primers of nptII and hpt genes and resulted 700 bp and 500 bp for fragments of nptII and hpt, respectively. After selection, acclimatization and molecular analysis steps, the efficiency levels of transformation of four maize lines were low, ranging from 3.8% to 12.8%. The level of transformation efficiency of ST-27 line was the highest accounting for 12.8% of 45 planted embryos on selection medium based on PCR analysis using specific primer for nptII gene. Overall, A. tumefaciensmediated in planta transformation on maize floral pistil in this study proved to be successful and rapid. Therefore, this enhanced transformation method will be beneficial for Indonesian maize genetic engineering.

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An efficient and rapid regeneration via multiple shoot induction from mature seed derived embryogenic and organogenic callus of Indian maize (Zea maysL.)

Cited by 21 publications

References 32 publications

Coleoptilar node - A season-independent explant source for in vitro culture in maize (Zea mays L.)

Coleoptilar node - A season-independent explant source for in vitro culture in maize (Zea mays L.)

In Vitro Selection of Local Maize (Zea mays) on NaCl Stress and Its Genetic Characterization using RAPD

Agrobacterium tumefaciens-MEDIATED IN-PLANTA TRANSFORMATION OF INDONESIAN MAIZE USING pIG121Hm-Cs PLASMID CONTAINING nptII AND hpt GENES

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