An efficient and reproducible protocol for the production of salt tolerant transgenic wheat plants expressing the<i>Arabidopsis AtNHX1</i>gene

Moghaieb, Reda E. A.; Sharaf, Ahmed; Soliman, Mohamed H.; Elarabi, Nagwa I.; Momtaz, Osama A.

doi:10.4161/gmcr.28941

Cited by 20 publications

(9 citation statements)

References 24 publications

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“…These results were largely consistent with the results obtained by expressing other Arabidopsis NHX genes, such as AtNHX1 (Chen et al, 2008;Leidi et al, 2010;Zhou et al, 2011;Banjara et al, 2012;Moghaieb et al, 2014).…”

Section: Discussionsupporting

confidence: 90%

“…Furthermore, atnhx1 and atnhx2 double knockout mutants displayed high sensitivity to salt stress, whereas overexpression of these genes increased the salt tolerance of the plants (Apse et al, 1999;Yokoi et al, 2002;Bassil et al, 2011b;Barragán et al, 2012). Interestingly, ectopic expression of AtNHX1 can enhance the salt stress tolerance of other plants, such as tomato, kiwifruit, wheat, tobacco, buckwheat, and peanut (Chen et al, 2008;Leidi et al, 2010;Zhou et al, 2011;Banjara et al, 2012;Moghaieb et al, 2014). These studies suggested that salt tolerance of crops could be improved through ectopic overexpression of NHXs by genetic transformation.…”

Section: Introductionmentioning

confidence: 99%

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Ectopic expression of Arabidopsis thaliana Na+(K+)/H+ antiporter gene, AtNHX5, enhances soybean salt tolerance

Liu

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Drought and salt stresses are the two major factors influencing the yield and quality of crops worldwide. Na+ antiporters (NHXs) are ubiquitous membrane proteins that play important roles in maintaining the cellular pH and NaThe model plant Arabidopsis potentially encodes six NHX genes, namely AtNHX1 to 6. In the present study, AtNHX5, a comparatively less well-studied NHX, was cloned and transferred into a soybean variety, Dongnong-50, via Agrobacterium-mediated cotyledonary node transformation to assess its role in improving salt tolerance of the transgenic plants. The transgenic soybean plants were tolerant to the presence of 300 mM NaCl whereas the non-transgenic plants were not. Furthermore, after NaCl treatment, the transgenic plants had a higher content of free proline but lower content of malondialdehyde compared to the non-transgenic plants. Our results revealed that that AtNHX5 possibly functioned by efficiently transporting Na + and K + ions from the roots to the leaves. Overall, the results obtained in this study suggest that soybean salt tolerance could be improved through the over expression of Arabidopsis AtNHX5.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Ectopic expression of Arabidopsis thaliana Na+(K+)/H+ antiporter gene, AtNHX5, enhances soybean salt tolerance

Liu

et al. 2016

Genet. Mol. Res.

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“…It is likely that UCB1 is more efficient at vacuolar sequestration due to the increased activity of salt ion antiporters such as the NHX transporter family (Gupta and Huang, 2014;Bassil et al, 2019;Guo et al, 2020). Overexpression of NHX1 increases salt tolerance in diverse species such as wheat, rice, tomato and mung bean (Zhang and Blumwald, 2001;Moghaieb et al, 2014;Kumar et al, 2017;Zeng et al, 2018) and likely contributes to vacuolar sequestration in pistachio. Retention of Na + in the vacuole is also regulated by the control of Na +permeable vacuolar channels, which mediate the back-leak of Na + into the cytosol (Isayenkov et al, 2010;Munns et al, 2016;Munns et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Root vacuolar sequestration and suberization contribute to salinity tolerance in Pistacia spp. rootstocks

Zhang¹,

Quartararo²,

Betz³

et al. 2020

Preprint

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Pistachio (Pistacia spp.) is a tree nut crop with relatively high salinity tolerance. Currently, limited information exists on its rootstock's cellular responses to salinity stress, especially in its roots. In this study, we investigated salinity tolerance at cellular level, in two pistachio rootstocks, Pistacia integerrima (PGI) and a hybrid, P. atlantica x P. integerrima (UCB1). Root tip sections were categorized across a developmental gradient according to their xylem development, and their sodium content and suberin deposition were analyzed with fluorescence microscopy. Our data demonstrated a correlation between vacuolar sequestration of sodium ions (Na+) and salinity tolerance in the UCB1 genotype. In addition, UCB1 displayed higher basal levels of suberization in both the exodermis and endodermis that increased further after salinity stress. Notably, the root region immediately distal to the region of secondary xylem initiation showed the highest amount of vacuolar Na+ sequestration, indicating a developmental regulation of this process. Our cumulative data demonstrate that salinity tolerance in pistachio rootstock species is associated with both vacuolar Na+ sequestration and suberin deposition at apoplastic barriers, and both are correlated with a root developmental gradient. These cellular characteristics are phenotypes that can be screened during the selection for salinity tolerant woody plant species.

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“…AtNHX1 is a vacuolar-localized NHX protein that is important for osmotic regulation and plant development. Overexpression of AtNHX1 increases the salt tolerance of plant species [11][12][13]. For example, two NHXs (HtNHX1 and HtNHX2) were isolated from a salt-tolerant plant Helianthus tuberosus.…”

Section: Introductionmentioning

confidence: 99%

Identification of NHXs in Gossypium species and the positive role of GhNHX1 in salt tolerance

et al. 2020

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Background: Plant Na + /H + antiporters (NHXs) are membrane-localized proteins that maintain cellular Na + /K + and pH homeostasis. Considerable evidence highlighted the critical roles of NHX family in plant development and salt response; however, NHXs in cotton are rarely studied. Results: The comprehensive and systematic comparative study of NHXs in three Gossypium species was performed. We identified 12, 12, and 23 putative NHX proteins from G. arboreum, G. raimondii, and G. hirsutum, respectively. Phylogenetic study revealed that repeated polyploidization of Gossypium spp. contributed to the expansion of NHX family. Gene structure analysis showed that cotton NHXs contain many introns, which will lead to alternative splicing and help plants to adapt to high salt concentrations in soil. The expression changes of NHXs indicate the possible differences in the roles of distinct NHXs in salt response. GhNHX1 was proved to be located in the vacuolar system and intensively induced by salt stress in cotton. Silencing of GhNHX1 resulted in enhanced sensitivity of cotton seedlings to high salt concentrations, which suggests that GhNHX1 positively regulates cotton tolerance to salt stress. Conclusion: We characterized the gene structure, phylogenetic relationship, chromosomal location, and expression pattern of NHX genes from G. arboreum, G. raimondii, and G. hirsutum. Our findings indicated that the cotton NHX genes are regulated meticulously and differently at the transcription level with possible alternative splicing. The tolerance of plants to salt stress may rely on the expression level of a particular NHX, rather than the number of NHXs in the genome. This study could provide significant insights into the function of plant NHXs, as well as propose promising candidate genes for breeding salt-resistant cotton cultivars.

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An efficient and reproducible protocol for the production of salt tolerant transgenic wheat plants expressing theArabidopsis AtNHX1gene

Cited by 20 publications

References 24 publications

Ectopic expression of Arabidopsis thaliana Na+(K+)/H+ antiporter gene, AtNHX5, enhances soybean salt tolerance

Ectopic expression of Arabidopsis thaliana Na+(K+)/H+ antiporter gene, AtNHX5, enhances soybean salt tolerance

Root vacuolar sequestration and suberization contribute to salinity tolerance in Pistacia spp. rootstocks

Identification of NHXs in Gossypium species and the positive role of GhNHX1 in salt tolerance

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