An efficient and robust fatty acid profiling method for plasma metabolomic studies by gas chromatography–mass spectrometry

Chiu, Huai-Hsuan; Tsai, Sung-Jeng; Tseng, Yufeng J.; Wu, Ming–Shiang; Liao, Wei‐Chih; Huang, Chiun‐Sheng; Kuo, Ching‐Hua

doi:10.1016/j.cca.2015.09.028

Cited by 16 publications

(10 citation statements)

References 35 publications

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“…The intra‐ and inter‐day accuracies (Table ) were assessed by the ratio of the measured concentration versus a known, spiked concentration, and these values were 90.51–109.59 and 94.57–109.33%, respectively. The precision and reproducibility were comparable with recent work using GC–MS .…”

Section: Resultssupporting

confidence: 85%

“…spiked concentration, and these values were 90.51-109.59 and 94.57-109.33%, respectively. The precision and reproducibility were comparable with recent work using GC-MS [14,20].…”

Section: Performance Of Gc-ms Methodssupporting

confidence: 84%

“…A good linear relationship was obtained for each FAME with a correlation coefficient ( R 2 ) around 0.999. The LOQs of all FAMEs varied from 0.0021 to 0.0720 μg/mL and were lower than some recent studies . The linear range was over three orders of magnitude.…”

Section: Resultscontrasting

confidence: 53%

“…Acid‐catalyzed methods are used for methylating all FAs (esterified and non‐esterified fatty acids), while base‐catalyzed methods are not suitable for free fatty acids. Frequently used reagents for acid‐catalyzed methods include boron trifluoride (BF 3 ), hydrochloric acid (HCl), sulfuric acid (H 2 SO 4 ) and acetyl chloride .…”

Section: Introductionmentioning

confidence: 99%

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Fatty acid profiling of blood cell membranes by gas chromatography with mass spectrometry

Liu

Weng

et al. 2016

J. Sep. Science

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Fatty acids, which are well-known for their influence on human metabolism and signal transduction, are also a substantial component of cellular membranes and regulate the basic properties and functions of membranes. Owing to their multiple functions, fatty acid profiles of cell membranes are of great interest to those who are studying the relationship between membrane biochemical compositions and functions. A HCl-catalyzed derivation method and a gas chromatography with mass spectrometry analysis method were developed to accurately profile the fatty acids in cell membranes of erythrocytes, leukocytes, and platelets. The detection limits of all 35 fatty acids ranged from 0.58 to 22 ng/mL and the limits of quantitation were between 2.1 and 72 ng/mL. Finally, the established method was used to profile the membrane fatty acids of 44 healthy volunteers from the north and south of China. Results revealed significant differences in the fatty acid profiles from the two regions, particularly those of the erythrocytes. This technique may be applied to cell membrane studies to generate new biological hypotheses concerning fatty acid composition and membrane functions as well as to construct related disease profiles.

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Section: Resultssupporting

confidence: 85%

“…spiked concentration, and these values were 90.51-109.59 and 94.57-109.33%, respectively. The precision and reproducibility were comparable with recent work using GC-MS [14,20].…”

Section: Performance Of Gc-ms Methodssupporting

confidence: 84%

Section: Resultscontrasting

confidence: 53%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Fatty acid profiling of blood cell membranes by gas chromatography with mass spectrometry

Liu

Weng

et al. 2016

J. Sep. Science

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“…Likewise, Jobard et al [ 134 ] identified a set of nine metabolites (histidine, acetoacetate, glycerol, pyruvate, N-acetyl glycoproteins (NAC 1 and 2), mannose, glutamate, and phenylalanine) associated with advanced metastatic disease. Three fatty acids (C22:0, C24:0, and C18:2n6 - namely behenic acid, lignoceric acid, and linoleic acid, respectively) were identified to be significantly reduced in plasma of premenopausal and postmenopausal breast cancer patients [ 135 ]. Yet another study suggested mainly two markers, lysophosphatidylethanolamine and ceramide, to play an important role in invasive ductal carcinoma [ 136 ].…”

Section: Blood-based Metabolome Analyses and Biomarkersmentioning

confidence: 99%

On metabolic reprogramming and tumor biology: A comprehensive survey of metabolism in breast cancer

et al. 2016

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Altered metabolism in tumor cells has been a focus of cancer research for as long as a century but has remained controversial and vague due to an inhomogeneous overall picture. Accumulating genomic, metabolomic, and lastly panomic data as well as bioenergetics studies of the past few years enable a more comprehensive, systems-biologic approach promoting deeper insight into tumor biology and challenging hitherto existing models of cancer bioenergetics. Presenting a compendium on breast cancer-specific metabolome analyses performed thus far, we review and compile currently known aspects of breast cancer biology into a comprehensive network, elucidating previously dissonant issues of cancer metabolism. As such, some of the aspects critically discussed in this review include the dynamic interplay or metabolic coupling between cancer (stem) cells and cancer-associated fibroblasts, the intratumoral and intertumoral heterogeneity and plasticity of cancer cell metabolism, the existence of distinct metabolic tumor compartments in need of separate yet simultaneous therapeutic targeting, the reliance of cancer cells on oxidative metabolism and mitochondrial power, and the role of pro-inflammatory, pro-tumorigenic stromal conditioning. Comprising complex breast cancer signaling networks as well as combined metabolomic and genomic data, we address metabolic consequences of mutations in tumor suppressor genes and evaluate their contribution to breast cancer predisposition in a germline setting, reasoning for distinct personalized preventive and therapeutic measures. The review closes with a discussion on central root mechanisms of tumor cell metabolism and rate-limiting steps thereof, introducing essential strategies for therapeutic targeting.

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Metabolomic Strategies Involving Mass Spectrometry Combined with Liquid and Gas Chromatography

Lopes

Cruz

Sussulini

et al. 2017

Advances in Experimental Medicine and Biology

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Amongst all omics sciences, there is no doubt that metabolomics is undergoing the most important growth in the last decade. The advances in analytical techniques and data analysis tools are the main factors that make possible the development and establishment of metabolomics as a significant research field in systems biology. As metabolomic analysis demands high sensitivity for detecting metabolites present in low concentrations in biological samples, high-resolution power for identifying the metabolites and wide dynamic range to detect metabolites with variable concentrations in complex matrices, mass spectrometry is being the most extensively used analytical technique for fulfilling these requirements. Mass spectrometry alone can be used in a metabolomic analysis; however, some issues such as ion suppression may difficultate the quantification/identification of metabolites with lower concentrations or some metabolite classes that do not ionise as well as others. The best choice is coupling separation techniques, such as gas or liquid chromatography, to mass spectrometry, in order to improve the sensitivity and resolution power of the analysis, besides obtaining extra information (retention time) that facilitates the identification of the metabolites, especially when considering untargeted metabolomic strategies. In this chapter, the main aspects of mass spectrometry (MS), liquid chromatography (LC) and gas chromatography (GC) are discussed, and recent clinical applications of LC-MS and GC-MS are also presented.

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An efficient and robust fatty acid profiling method for plasma metabolomic studies by gas chromatography–mass spectrometry

Cited by 16 publications

References 35 publications

Fatty acid profiling of blood cell membranes by gas chromatography with mass spectrometry

Fatty acid profiling of blood cell membranes by gas chromatography with mass spectrometry

On metabolic reprogramming and tumor biology: A comprehensive survey of metabolism in breast cancer

Metabolomic Strategies Involving Mass Spectrometry Combined with Liquid and Gas Chromatography

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