An efficient and sensitive method on the identification of unsaturated fatty acids in biosamples: Total lipid extract from bovine liver as a case study

Mao, Rui; Li, Wei; Jia, Penghao; Ding, Hui; Zhang, Lin; Fu, Zhifei; Fu, Xuebin; Kaushal, Sunjay; Dou, Zhiying; Han, Lifeng

doi:10.1016/j.chroma.2022.463176

Cited by 5 publications

(2 citation statements)

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Section: Derivatization Of Unsaturated Fatty Acids (Ufas)mentioning

confidence: 99%

“…Most recently, to overcome the low reactive rates of P–B derivatization reagents and the low MS response of P–B products, Mao et al compared a set of P–B derivatization reagents and concluded that 3-pyridinecarboxaldehyde (3-PYA) ( Figure 9 ) leads to high performance [ 90 ]. This reagent was successfully applied for either the shotgun or LC-MS analysis of UFAs in a total lipid extract from bovine liver [ 90 ]. Most recently, Han et al developed new P–B derivatization reagents, namely benz[g]isoquinoline-5,10-dione (BIQD) and 6,9-difluorobenzo[g]isoquinoline-5,10-dione (DF-BIQD) ( Figure 9 ), which allow irradiation by visible-light at 405 nm and compact fluorescent lamp visible-light at >400 nm [ 91 ].…”

Section: Derivatization Of Unsaturated Fatty Acids (Ufas)mentioning

confidence: 99%

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Liquid Chromatography-Mass Spectrometry (LC-MS) Derivatization-Based Methods for the Determination of Fatty Acids in Biological Samples

Mantzourani

Kokotou

2022

Molecules

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Fatty acids (FAs) play pleiotropic roles in living organisms, acting as signaling molecules and gene regulators. They are present in plants and foods and may affect human health by food ingestion. As a consequence, analytical methods for their determination in biological fluids, plants and foods have attracted high interest. Undoubtedly, mass spectrometry (MS) has become an indispensable technique for the analysis of FAs. Due to the inherent poor ionization efficiency of FAs, their chemical derivatization prior to analysis is often employed. Usually, the derivatization of the FA carboxyl group aims to charge reversal, allowing detection and quantification in positive ion mode, thus, resulting in an increase in sensitivity in determination. Another approach is the derivatization of the double bond of unsaturated FAs, which aims to identify the double bond location. The present review summarizes the various classes of reagents developed for FA derivatization and discusses their applications in the liquid chromatography-MS (LC-MS) analysis of FAs in various matrices, including plasma and feces. In addition, applications for the determination of eicosanoids and fatty acid esters of hydroxy fatty acids (FAHFAs) are discussed.

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Section: Derivatization Of Unsaturated Fatty Acids (Ufas)mentioning

confidence: 99%