An efficient approach for the isolation, identification and evaluation of antimicrobial plant components on an analytical scale, demonstrated by the example of Radix imperatoriae

Abstract

Using Radix imperatoriae (the root of masterwort) as an example, we describe an efficient approach for the isolation, identification and evaluation of bioactive plant components on an analytical scale. The extraction of Radix imperatoriae with ethyl acetate was enhanced by the application of ultrasound oscillations. This rhizome extract was applied to three pathogenic bacteria (Bacillus cereus, Escherichia coli, and Staphylococcus aureus) to determine its antimicrobial activity. Disk diffusion was utilized to determine susceptibility. The extract components were separated using a series of chromatography approaches (semi-preparative RP-HPLC, or RP-HPLC on an analytical scale), followed by testing. All fractions were analyzed by LC-UV-ESI-MS and 600 MHz microcoil 1 H NMR spectroscopy. Among other findings, in the fraction with the highest antibacterial activity we were able to identify oxypeucedanin and oxypeucedanin hydrate. Subsequent analysis revealed that only oxypeucedanin hydrate had antibacterial activity, whereas oxypeucedanin itself was inactive at the concentrations applied. Furthermore, oxypeucedanin hydrate appears to be largely, or exclusively, a by-product of sample preparation, since it is either not synthesized by the plant as a second metabolite or is produced by it in only very small quantities.

Autophagy, Apoptosis, Mitoptosis and Necrosis: Interdependence Between Those Pathways and Effects on Cancer

“…Beside environmental factors, viral infections and life-style are responsible factors for its increasing frequency (Alavian et al 2011;Gurevich-Panigrahi et al 2009). Natural products are a frequent inspiration for the development of new anti-cancer drugs (Ghavami et al 2010;Gokay et al 2010;Panigrahi et al 2012). As outlined above, much attention in recent years has been paid to experimental drugs that modulate autophagy or apoptosis.…”

Phytochemical Investigation, Isolation and Characterization of Coumarins from Aerial Parts and Roots of Tunisian Pituranthos chloranthus (Apiaceae)

“…The structures of these compounds were elucidated by spectroscopic methods ( 1 H-NMR, 13 CNMR, DEPT, HMBC, HSQC, COSY) and HR-MS as well as by comparison of their NMR data with those reported in the literature. [14][15][16][17][18][19][20] Compound ( 14 In addition, a survey of the literature showed that this compound has been isolated from the shoots 4 and the roots 15 of Pituranthos triradiatus. However, this is the first time that this compound was isolated from P. Chloranthus.…”

Possible Intestinal Absorption Enhancers fromCitrus hystrix

“…The fractions were further isolated by PTLC to obtain pure compounds. Five furanocoumarins were elucidated and identified by LC-MS and 1 H NMR as follows: bergamottin (C21H22O4, MW 338), 6',7'-dihydroxybergamottin (C21H24O6, MW 372), 6',7'-epoxybergamottin (C21H22O5, MW 354) oxypeucedanin (C16H14O5, MW 286), oxypeucedanin hydrate (C16H16O6, MW 304) [10][11][12][13][14].…”