An efficient, cost-effective method for determining the growth rate of sulfate-reducing bacteria using spectrophotometry

Wood, Jennifer L.; Osman, A.; Wade, Scott A

doi:10.1016/j.mex.2019.09.036

Cited by 21 publications

(11 citation statements)

References 28 publications

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“…Cell density (absorbance) was measured with a UV-6100 spectrophotometer at 600 nm (OD 600 nm) at approximately 1 h intervals for up to 15 h [50]. The exponential stage was used to determine µ from the natural optical density logarithm of two data points versus the time corresponding to the two-point OD data [51]. In the following step, the growth rate of the bacterial isolated was calculated by Equation (1).…”

Section: Bacterial Growth Rate and Growth Curvementioning

confidence: 99%

An Assessment of the Lactic Acid-Producing Potential of Bacterial Strains Isolated from Food Waste

Uwamahoro

Timilsina

et al. 2022

Microbiology Research

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Lactic acid (LA) is widely used in many industries as a crucial starting material in food products, bio-based materials, and biodegradable polymers. The goals of this research were to isolate LA bacteria from food wastes, assess their potential for LA production, and study their growth characteristics. In this study, six bacterial strains were isolated from food waste and identified using 16S rRNA gene sequencing; namely, Weissella viridescens WJ39, Leuconostoc lactis YS33, Leuconostoc citreum KD42, Leuconostoc mesenteroides VN60, Macrococcus caseolyticus FCI29, and Weissella confusa RG41. W. viridescens WJ39 showed the highest potential for lactic acid production (17.56 g L−1day−1), and the lowest potential was found in L. lactis YS33 (14.09 g L−1day−1). There were significant differences (p < 0.05) in the LA production rates among Weissella spp., Leuconostoc spp., and Macrococcus spp. Moreover, dramatic differences in growth rate were observed among the six strains. W. viridescens WJ39 exhibited the highest growth rate (0.80 h−1), while M. caseolyticus FCI29 exhibited the lowest growth rate (0.57 h−1). W. viridescens WJ39 also exhibited lactic acid production (at a rate around 2 g L−1day−1) in a lab incubation experiment with food waste as a nutrient source. The draft genome of W. viridescens WJ39 with 16 contigs was constructed with an N50 of 215217 bp. The genome size was approximately 1.54 Mb, with a GC content of 41%. A hicD gene, known to catalyze the conversion of pyruvate to D-lactate, was discovered in the genome. This study illustrated the potential for the production of lactic acid from food waste with lactic acid bacteria.

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Section: Bacterial Growth Rate and Growth Curvementioning

confidence: 99%

An Assessment of the Lactic Acid-Producing Potential of Bacterial Strains Isolated from Food Waste

Uwamahoro

Timilsina

et al. 2022

Microbiology Research

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“…Wood's research group has carried out more advancement in the determination of SRB growth rate applying spectrophotometry. [32] The aforementioned Bernardez's method of SRB estimation has limitation while using aliquot volumes around 45 mL that confine time points for sampling and extensive time for cell preparation before (optical density) OD reading. In upgraded method, authors incorporated lower sample volumes using Bernardez's approach to high throughput microtiter plate method which in turn enhanced the efficiency and practical application of this method.…”

Section: Uv Visible Methodsmentioning

confidence: 99%

Turning the Page: Advancing Detection Platforms for Sulfate Reducing Bacteria and their Perks

Asif

Aziz

Ashraf

et al. 2021

The Chemical Record

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Sulfate reducing bacteria (SRB) are blamed as main culprits in triggering huge corrosion damages by microbiologically influenced corrosion. They obtained their energy through enzymatic conversion of sulfates to sulfides which are highly corrosive. However, conventional SRB detection methods are complex, time‐consuming and are not enough sensitive for reliable detection. The advanced biosensing technologies capable of overcoming the aforementioned drawbacks are in demand. So, nanomaterials being economical, environmental friendly and showing good electrocatalytic properties are promising candidates for electrochemical detection of SRB as compared with antibody based assays. Here, we summarize the recent advances in the detection of SRB using different techniques such as PCR, UV visible method, fluorometric method, immunosensors, electrochemical sensors and photoelectrochemical sensors. We also discuss the SRB detection based on determination of sulfide, typical metabolic product of SRB.

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“…Growth kinetics to establish the relationship between growth rate and nutrients present in the isolates were done as per universally practiced methods [8]. In brief, isolates retrieved from glycerol stock stored at −80 °C in Mueller-Hinton broth (MHB) and incubated at 37 °C with continuous shaking at 250 r.p.m.…”

Section: Growth Kineticsmentioning

confidence: 99%

Virulence gene mutations as a differentiator of clinical phenotypes: insights from community-acquired uropathogenic Escherichia coli

et al. 2022

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Uropathogenic Escherichia coli (UPEC) remains an important cause of urinary tract infection during pregnancy. Multiple molecular virulence determinants and antibiotic resistant genes facilitate its pathogenesis and virulence phenotype. Hence it is hypothesized that there will be considerable variation in genes among the isolates from symptomatic as well as asymptomatic bacteriuria (ABU) during pregnancy. The aim of this study was to decipher the genetic variation among the two phenotypes. Six different UPEC isolates collected from urine specimens of consecutive pregnant females (five, symptomatic bacteriuria and one, ABU) were tested for their growth kinetics, and biofilm formation. A total of 87 virulence determinants and 56 antibiotic resistance genes were investigated using whole-genome sequencing, to identify putative drives of virulence phenotype. In this analysis, we identified eight different types of fully functional toxin antitoxin (TA) systems [HipAB, YefM-YoeB, YeeU-YeeV (CbtA), YhaV-PrlF, ChpBS, HigAB, YgiUT and HicAB] in the isolates from symptomatic bacteriuria; whereas partially functional TA system with mutations were observed in the asymptomatic one. Isolates of both the groups showed equivalent growth characteristics and biofilm-formation ability. Genes for an iron transport system (Efe UOB system, Fhu system except FhuA) were observed functional among all symptomatic and asymptomatic isolates, however functional mutations were observed in the latter group. Gene YidE was observed predominantly associated with the biofilm formation along with few other genes (BssR, BssS, YjgK, etc.). This study outlines putative critical relevance of specific variations in the genes for the TA system, biofilm formation, cell adhesion and colonization among UPEC isolates from symptomatic and asymptomatic bacteriuria among pregnant women. Further functional genomic study in the same cohort is warranted to establish the pathogenic role of these genes.

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An efficient, cost-effective method for determining the growth rate of sulfate-reducing bacteria using spectrophotometry

Abstract: Graphical abstract

Cited by 21 publications

References 28 publications

An Assessment of the Lactic Acid-Producing Potential of Bacterial Strains Isolated from Food Waste

An Assessment of the Lactic Acid-Producing Potential of Bacterial Strains Isolated from Food Waste

Turning the Page: Advancing Detection Platforms for Sulfate Reducing Bacteria and their Perks

Virulence gene mutations as a differentiator of clinical phenotypes: insights from community-acquired uropathogenic Escherichia coli

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