An efficient liposomal gene delivery vehicle using Sendai F/HN proteins and protamine

Kim, H S; Kim, J S; Lee, Y K; Koo, Kyung Hye; Park, Yu Shin

doi:10.1038/sj.cgt.7701121

Cited by 17 publications

(16 citation statements)

References 36 publications

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“…This is especially important for the delivery of more labile drugs like genes and peptides. The use of cationic lipids and polymers for intracellular drug delivery by facilitating endosomal escape is well documented 131 . Lipids such as DOTMA and DOTAP as well as branched polymers like polyethyleneimine and PAMAM dendrimers have been used extensively for their ability to fuse with the endosomal membrane thus dispelling their contents into the cytoplasm 132 .…”

Section: Biological Barriersmentioning

confidence: 99%

Barriers to drug delivery in solid tumors

Sriraman

Aryasomayajula

Torchilin³

2014

Tissue Barriers

261

157

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Over the last decade, significant progress has been made in the field of drug delivery. The advent of engineered nanoparticles has allowed us to circumvent the initial limitations to drug delivery such as pharmacokinetics and solubility. However, in spite of significant advances to tumor targeting, an effective treatment strategy for malignant tumors still remains elusive. Tumors possess distinct physiological features which allow them to resist traditional treatment approaches. This combined with the complexity of the biological system presents significant hurdles to the site-specific delivery of therapeutic drugs. One of the key features of engineered nanoparticles is that these can be tailored to execute specific functions. With this review, we hope to provide the reader with a clear understanding and knowledge of biological barriers and the methods to exploit these characteristics to design multifunctional nanocarriers, effect useful dosing regimens and subsequently improve therapeutic outcomes in the clinic.

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Section: Biological Barriersmentioning

confidence: 99%

Barriers to drug delivery in solid tumors

Sriraman

Aryasomayajula

Torchilin³

2014

Tissue Barriers

261

157

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“…Taken into account this point, we selected chitosan nanoparticles, a non-viral vector, in the present study. Non-viral vectors include liposomes [7][8][9] , composites [10] , microspheres [11] , and nanoparticles [12,13] , but the cytotoxicity of the liposomes limits its application in vivo. Owing to its loose constitution, the constancy of composites is poor.…”

Section: Introductionmentioning

confidence: 99%

Expression of human insulin gene wrapped with chitosan nanoparticles in NIH3T3 cells and diabetic rats

Niu

Xie

et al. 2008

Acta Pharmacol Sin

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Aim: To study the expression of human insulin gene wrapped with chitosan nanoparticles in NIH3T3 cells and diabetic rats. Methods: pCMV.Ins, an expression plasmid of the human insulin gene, was constructed. In total, 100 μg pCMV.Ins wrapped with chitosan nanoparticles (chitosan-pCMV.Ins) was transfected to NIH3T3 cells and diabetes rats through lavage and coloclysis, respectively. The transfected cells were grown in Dulbecco's modified Eagle's medium, containing G418, for 72 h after transfection. The clones were selected and continued to grow in G418 medium for 24 d. The expression of human insulin was detected by immunohistochemistry. Human insulin in the culture medium of transfected cells was measured. Fasting blood glucose and plasma human insulin of diabetic rats were measured for 5 d after transfection. RT-PCR and Western blotting were performed to confirm the expression of the human insulin gene in diabetic rats. Results: Approximately 10% of NIH3T3 cells transfected by chitosan-pCMV.Ins expressed human insulin. Human insulin in the culture medium of NIH3T3 cells transfected by chitosan-pCMV.Ins significantly increased compared with that of the control group (P<0.01). Fasting blood glucose levels of the lavage group and the coloclysis group decreased significantly in 5 d (P<0.01) in comparison, while plasma insulin levels were much higher (P<0.01). The human insulin gene mRNA and human insulin were only detected in the lavage and the coloclysis groups. Conclusion: The human insulin gene can be transfected and expressed successfully by chitosanpCMV.Ins in NIH3T3 cells and diabetes rats, which indicates that chitosan is a promising, non-viral vector for gene expression.

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“…In this context, it should be emphasized that Bombelli et al and Kim et al observed a similar correlation between transfection efficiency and extent of DNA condensation, using cationic gemini surfactants and protamine, respectively, as DNA condensing agents [40,41]. On the other hand, Farago et al reported an inverse correlation between the packaging of CL/DNA complexes and their transfection efficiency using multivalent cationic lipids [42].…”

Section: Discussionmentioning

confidence: 84%