With the development of virtual reality application in the medical field, two-dimensional medical image of the three-dimensional visualization technology made possible. Surgery gets into minimally invasive operation microscopy Era, and gradually becomes a new research hotspot. This paper studies the realization of two-dimensional medical image 3D reconstruction visualization system method, and the overall process and management module. Using the main technology of VTK (The Visualization Toolkit) to achieve a two-dimensional medical image three-dimensional visualization system, which can help the physician to obtain help clinical diagnosis Information and play an important role in treatment, accurate positioning in diseased tissue and tumor early diagnosis.