An efficient method based on an inhibitor–enzyme complex to screen an active compound against lipase from Toona sinensis

Abstract

As a popular vegetable, Toona sinensis has a wide range of bioactivities including lipase inhibitory activity. In the present study, an efficient and rapid method using ligand-enzyme complex was established...

Evaluation of pancreatic lipase and angiotensin-converting enzyme inhibitors from ethanolic extract of butterfly pea (Clitoria ternatea L.) flowers

“…The in vitro activity assay revealed that the butterfly pea flower (CT) extract inhibits lipase with an IC50 value of 131.7867 ± 5.27 ppm. This value was lower than that for the in vitro activity test of Toona sinensis, with an IC50 value of 118.8 ± 1.53 µg/mL [28], and for the methanolic extract of Hibiscus sabdariffa L. (Malvaceae), with an IC50 value of 35.8 ± 0.8 µg/mL, yet it was higher than the IC 50 value of 152.0 ± 7.0 µg/mL for the methanolic extract of Tamarindus indica L. (Leguminosae) [30].…”

“…The lipase inhibition test method uses ρ-NPB, which acts as a substrate, with the addition of a lipase catalyst to produce butyrate products and ρ-nitrophenolate ions with a yellow color [28]. The antilipase activity test was carried out according to the results of optimization: 30 min of incubation time, 3 mg/mL substrate concentration, and 0.025 mg/mL enzyme concentration.…”

Leaves of Cedrela sinensis Attenuate Chronic Unpredictable Mild Stress-Induced Depression-like Behavior via Regulation of Hormonal and Inflammatory Imbalance

“…In this study, the exposure of chronic stress caused depletion of monoamine hormones; however, EFCS increased the levels of dopamine, tryptophan, 5-HT, and melatonin ( Figure 6 ). It has been reported that Cedrela sinensis leaf is rich in hormonal precursor amino acids, such as tryptophan, tyrosine, and glutamic acid, that are associated with stress reduction [ 74 , 75 ]. These amino acids are absorbed into the body and converted to serotonin, melatonin, dopamine, and γ-aminobutyric acid (GABA), and they suppress stress and help to suppress the imbalance of the HPA axis [ 72 , 73 ].…”

Identification of yeast α-glucosidase inhibitors from Pueraria lobata by ligand fishing based on magnetic mesoporous silicon combined with knock-out/knock-in technology

“…The inhibitory kinetics of puerarin, daidzin, and daidzein against α -glucosidase was tested based on the reported enzyme activity determination method. 11 First, in the presence of inhibitors, different concentrations of α -glucosidase were reacted with the same concentration of p -NPG. Subsequently, the amount of p -NPG was changed, while the amount of α -glucosidase remained unchanged.…”