An Efficient Method for Qualitative Screening of Ligninolytic Enzyme Potential of Ganoderma lucidum

Kaur, Harsimran; Kapoor, Smita; Sharma, Shivani

doi:10.20546/ijcmas.2018.708.247

Cited by 6 publications

(5 citation statements)

References 25 publications

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“…Based on the results of the ligninolytic potency index measurement, isolate CSF#5 produced the largest potency index on the 3rd and 7th day of observation, followed by isolates CSF#15, CSF#10 and CSF#21. A strain is categorized as having a high ligninolytic potential index in the range of values (PI > 1,000), while a low ligninolytic potential index in the range of values (PI < 1,000) [14]. Fungi with a lower potency index require a longer adaptation to produce enzymes to be oxidized [17] [18].…”

Section: Screening Results and Enzyme Potency Index Of Ligninolytic A...mentioning

confidence: 99%

“…Fungal cultures were inoculated onto 1% Carboxymethyl Cellulose (CMC) media and incubated for 3-7 days at room temperature. Then the petri dish was soaked with 15 ml of 0.1% Congo red solution and let stand for 30 minutes, then rinsed twice using 15 ml of 1 N NaCl and let stand for 15 minutes [14]. A positive reaction was indicated by the presence of a clear zone around the colony which was an indication of cellulase enzyme activity.…”

Section: Ligninolytic Activity Test 24 Cellulolytic Activity Testmentioning

confidence: 99%

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Characterization of lignocellulolytic fungi isolated from cocoa rhizosphere under different cropping patterns

Nirmalasari

Santosa

Prastowo

2022

IOP Conf. Ser.: Earth Environ. Sci.

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Cocoa plantations may provide a considerable amount of fungal biodiversity with high potential as soil organic matter biodecomposer agents. Therefore, the isolation and characterization of potential fungi is the first step to obtain the wide variety of lignocellulolytic enzymes. This study aimed at identifying potential lignocellulolytic fungi isolated from different type cocoa cropping pattern. The lignocellulose-degrading enzymes was determined by growing the isolated fungi on Guaiacol containing PDA media, and CMC media with additional of Red Congo solution. Their potential index (IP) was scored by measuring the diameter of the clear zone around the fungal colonies. Our identifications suggest the presence of both ligninolytic, and cellulolytic enzymes through a reddish, and clear zones produced around the colonies. Additionally, we identified two types of potential fungi with higher potential index both ligninolytic and cellulolytic, including strain fungal CSF#5 and CSF#15 which belongs to the group of fungi Ascomycetes.

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Section: Screening Results and Enzyme Potency Index Of Ligninolytic A...mentioning

confidence: 99%

Section: Ligninolytic Activity Test 24 Cellulolytic Activity Testmentioning

confidence: 99%

Characterization of lignocellulolytic fungi isolated from cocoa rhizosphere under different cropping patterns

Nirmalasari

Santosa

Prastowo

2022

IOP Conf. Ser.: Earth Environ. Sci.

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“…Thus, this screening helps us to select the most promising fungal isolate. One of the observations showed reduced mycelium growth when incubated with guaiacol containing solid medium, which may be due to the changes in some metabolic activity of isolates during growth in selected media [23].…”

Section: Resultsmentioning

confidence: 99%

Isolation, identification, and optimization of laccase from Alternaria alternata

2020

jabb

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Laccase, lignin peroxidase, and manganese peroxidase have a synergistic effect on a wide range of recalcitrant compounds. Among them, laccase is polyphenol oxidase widely available in fungi, plant species, and insects. Laccase has an important role in effluent decoloration, detoxification of pulp bleaching, and bioremediation process. Screening was carried out to find new fungal isolate for the presence of laccase activity with guaiacol as indicator compound. Sixteen fungal isolates were obtained from biodeteriorated agro waste and the wood samples were collected from North Gujarat region of India. Among these isolates, one of the fungal isolates was observed with good laccase activity and identified as Alternaria alternata. Laccase activity was determined using 2,2'-azinobis-(3-ethylbenzethiazoline-6-sulfonate) as substrate. Various production parameters such as pH, temperature, various carbon sources, nitrogen source, inducers, and cations were used to select the optimum condition for further increase in the production of this enzyme. Maximum laccase activity was obtained with glucose and sucrose as carbon source, 0.2% ammonium sulfate as nitrogen source, and 0.06% Cu +2 with 1.5 mM veratryl alcohol as inducer under optimized condition.

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“…Strain selection was performed by monitoring the formation of hydrolysis halos on skimmed milk agar plates. The plates were inoculated by pitting in the center of the inverted Petri dishes and incubated in the same position at 30 • C for 72 h. The plates were observed every 24 h to visualize the formation of hydrolysis halos, and colony and hydrolysis halos diameters were recorded to calculate the potency index (PI) using Equation (1) [16].…”

Section: Strain Selectionmentioning

confidence: 99%

Effect of Glucose Concentration on the Production of Proteolytic Extract by Different Strains of Aspergillus under Solid-State Fermentation

Londoño-Hernández,

García-Gómez,

Huerta-Ochoa

et al. 2024

Fermentation

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Proteases are important enzymes because of their extended uses in several industries, such as food, beverages, pharmacy, detergents, and many others. Aspergillus is one of the most used fungi strains for enzyme production by solid-state fermentation (SSF). Disponibility of the carbon source is a key factor for protease production. In addition, the selection of solid support has great importance, as it must provide suitable airflow through the packed bed and nutrient diffusion inside the fermentable mass. Six Aspergillus strains and two inert supports (Agrolite (AL) and Polyurethane (PUF)) were tested for protease production from fish flour (FF) at different glucose concentrations (0, 5, 10, 15%) by SSF. The FF/PUF mixture at 70/30 (w/w) ratio, with 75.39% moisture, and a critical moisture point of 0.11 gH2O/g, presented a texture that allowed heat and mass transfer and provided enough moisture to make free water available as required for microorganism growth during the fermentation process. Aspergillus oryzae 2095 produced higher amounts of neutral and alkaline proteases with the addition of 5% glucose to the growth medium. Kinetics studies reveal that protease production is partially associated with growth. The extracts obtained can be used in different industries, and especially to prepare fish high-value by-product hydrolysates.

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An Efficient Method for Qualitative Screening of Ligninolytic Enzyme Potential of Ganoderma lucidum

Cited by 6 publications

References 25 publications

Characterization of lignocellulolytic fungi isolated from cocoa rhizosphere under different cropping patterns

Characterization of lignocellulolytic fungi isolated from cocoa rhizosphere under different cropping patterns

Isolation, identification, and optimization of laccase from Alternaria alternata

Effect of Glucose Concentration on the Production of Proteolytic Extract by Different Strains of Aspergillus under Solid-State Fermentation

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