An Efficient Method for the Extraction of Alkaloids from Bitter Lupin Seed

Abstract

Experimental conditions were worked out on a laboratory scale for the removal of alkaloids from seeds of bitter Peruvian lupin, Lupinus mutabilis. Four trials were carried out in this study using dilute aqueous solutions of HC1 and AcOH, and water. A simple und quick procedure is recommended for the removal of alkaloids from the whole seeds. The seeds are first soaked in 0.1N AcOH for 2hr, then boiled in water for 0.5hr, and eventually extracted with water for 8hr following the Soxhlet principle. More than 95% of the alkaloids are extracted by this procedure without leading to environmental pollution. The debittered whole seeds have a bland taste and contain around 50% protein and 20% fat Following Soxhlet extraction, the alkaloids are easily obtained as a side product.

Alkaloid content and taste profile assessed by electronic tongue of Lupinus albus seeds debittered by different methods

“…The better debittering capacity of saline solutions compared to water, evident also from the results of the solutions with sodium chloride, was likewise reported by Villacrés et al (2020). Furthermore, these results agree with Jiménez-Martínez et al (2010) and Karara (1987), who demonstrated greater debittering efficiency using, respectively, 0.1 M acetic acid and 0.1% citric acid solutions instead of pure water.…”

Using ultrasound for improving hydration and debittering of Andean lupin grains

“…(Carvajal‐Larenas, Van Boekel, Koziol, Nout, & Linnemann, ) and 3.1% d.b. (Karara, ). Further, the conventional hydration process reduced almost 45% of the grain alkaloid content, from 3.46 ± 0.16% d.b.…”