An efficient method of ovarian stimulation and in vitro embryo production from prepubertal lambs

Morton, Katherine; Catt, Sally; Maxwell, W.M.C.; Evans, G.

doi:10.1071/rd04105

Cited by 8 publications

(8 citation statements)

References 31 publications

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“…2005b). Yet, Morton et al. (2005a,b,c,d) reported similar rates of oocyte development in vitro when eCG was administered with first FSH injection.…”

Section: Hormone Stimulation Of Donorsmentioning

confidence: 93%

“…Oocyte development to the blastocyst stage is highly dependant on individual lambs (Ptak et al. 2003; Morton et al. 2005c), with the number of embryos produced per lamb ranging from 0 to 55 (Morton et al.…”

Section: Donor Selectionmentioning

confidence: 99%

“…Various methods have been studied for selecting lambs although most have been inconclusive. Body weight prior to hormone stimulation and growth rate are not related to ovarian weight or the number of ovarian follicles after hormone stimulation (Morton et al. 2005c).…”

Section: Donor Selectionmentioning

confidence: 99%

“…While Ptak et al. (1999, 2006) and Morton et al. (2005b,c,d) utilized oocytes from lambs of a similar age (4 weeks), the different breeds of the donors, hormone stimulation regimes (in particular, the FSH dose: 2.7 or 7 mg and 130 mg pFSH, respectively) and IVP systems confound comparisons between these studies.…”

Section: Development Of Oocytes From Prepubertal Animalsmentioning

confidence: 99%

“…1999). Increases in both the number and in vitro developmental competence of oocytes derived from very young donors have been reported recently (Morton et al. 2005c).…”

Section: Introductionmentioning

confidence: 98%

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Developmental Capabilities of Embryos Produced In Vitro from Prepubertal Lamb Oocytes

Morton

2008

Reprod Domestic Animals

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Breeding from prepubertal females, known as juvenile in vitro embryo transfer (JIVET), reduces the generation interval and increases the rate of genetic gain in animal breeding programs. While the birth of the first lambs from prepubertal ewes occurred nearly 30 years ago; and there is considerable interest in the commercialization of this technology, its efficiency remains too low. The advent of in vitro production (IVP) of embryo resulted in the more widespread use of JIVET. Morphologic and metabolic differences coupled with reduced in vitro and in vivo development of oocytes derived from prepubertal animals have been reported. Research has been undertaken to optimize donor selection and hormone stimulation methods in an attempt to reduce the variability and increase the proportion of donors responding to hormone stimulation and increase oocyte developmental competence. Yet, this variation persists and the development of oocytes and embryos from prepubertal animals remains reduced when compared with adults. Recent improvements to JIVET, resulting from a modified hormone stimulation regime, have eliminated the failure of donors to respond to hormone stimulation, and increased both the number and developmental competence of oocytes harvested from very young prepubertal lambs. This increased efficiency has facilitated the incorporation of other reproductive technologies such as sperm sexing with JIVET, resulting in the birth of lambs of a pre-determined sex from prepubertal lambs. Increased rates of genetic gain in sheep breeding programs can be achieved by combining sexed sperm with oocytes obtained from lambs as young as 3-4 weeks of age. Continued increases in the efficiency of JIVET resulting from further improvements to hormone stimulation regimes and an increased understanding of the differences between oocytes from adult and prepubertal animals will result in the commercialization of this technology.

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“…2005b). Yet, Morton et al. (2005a,b,c,d) reported similar rates of oocyte development in vitro when eCG was administered with first FSH injection.…”

Section: Hormone Stimulation Of Donorsmentioning

confidence: 93%

Section: Donor Selectionmentioning

confidence: 99%

Section: Donor Selectionmentioning

confidence: 99%

Section: Development Of Oocytes From Prepubertal Animalsmentioning

confidence: 99%

“…1999). Increases in both the number and in vitro developmental competence of oocytes derived from very young donors have been reported recently (Morton et al. 2005c).…”

Section: Introductionmentioning

confidence: 98%

See 3 more Smart Citations