An efficient micro-method of DNA isolation from mature leaves of four hardwood tree species Acer, Fraxinus, Prunus and Quercus

Lefort, François; Douglas, G. C.

doi:10.1051/forest:19990308

Cited by 152 publications

(104 citation statements)

References 6 publications

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“…Extração de DNA: amostras de folhas expandidas jovens foram coletadas durante a estação de crescimento e armazenadas a -20°C para posterior extração de DNA, utilizando-se do método descrito por Lefort & Douglas (1999), com modificações no tampão de extração (50mM Tris HCl pH 8,0; 20mM EDTA pH 8,0; 1,1M NaCl; 0,4M LiCl; 1% CTAB; 2% PVP40 e 0,5% Tween 20).…”

Section: Methodsunclassified

Uso prático de marcadores moleculares para seleção assistida no melhoramento de uvas de mesa apirênicas

et al. 2006

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RESUMO -A hipótese mais bem aceita atualmente para explicar a genética complexa da estenoespermocarpia, observada na videira, indica que a expressão deste fenótipo é controlada por três genes recessivos, independentemente herdados e controlados por um gene regulador dominante (sdI). Em estudo anterior, Lahogue et al. (1998) identificaram um marcador RAPD (Random Amplified Polymorphic DNA) ligado ao gene sdI e utilizaram-no para desenvolver um SCAR (Sequence Characterized Amplified Region) co-dominante denominado SCC8, que pode distinguir, em uma progênie, indivíduos com semente como também selecionar indivíduos apirênicos. Neste trabalho, são apresentados resultados da avaliação do potencial de aplicação do marcador molecular SCAR SCC8 para seleção assistida do caráter da apirenia no melhoramento de uvas de mesa sem sementes. A utilização deste marcador na seleção assistida para apirenia em uvas de mesa mostrou-se viável, e as conseqüências da sua utilização no programa de melhoramento da Embrapa Uva e Vinho são discutidas. Termos para indexação: Vitis, marcador molecular, seleção assistida, apirenia. PRACTICAL USE OF MOLECULAR MARKERS FOR SEEDLESSNESS ASSISTED SELECTION IN GRAPEVINE BREEDINGABSTRACT -The inheritance of seedlessness in grapevine is based on a complex genetic system, where the expression of three independently inherited recessive genes is controlled by a dominant regulator gene (sdI). In a previous study, Lahogue et al. (1998) identified a random amplified polymorphic DNA marker, tightly linked to the sdI gene and developed a codominant SCAR (Sequence Characterized Amplified Region) marker named SCC8, that allows the distinction of seeded and seedless plants in a segregating progeny. The aim of the present work was to evaluate the usefullness of the SCAR marker SCC8 for assisted selection of the seedlessness character in grape breeding. According to our results, the use of the SCC8 marker is economically viable and the consequences of its use in the grapevine breeding program at Embrapa Uva e Vinho are discussed.

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Section: Methodsunclassified

Uso prático de marcadores moleculares para seleção assistida no melhoramento de uvas de mesa apirênicas

et al. 2006

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“…Total genomic DNA was extracted from young leaves of "Isabel" and "Isabel Precoce" grape cultivars using a modified procedure adapted from [28] [29]. The genetic identity between the original cultivar "Isabel" and its EDV "Isabel Precoce" was verified by genotyping nine microsatellite loci: VVMD36, VVMD27, VVMD8, VVMD7, VVMD7, VVMD6, VVS2, VVS4, VRZAG62, VRZAG72 as described by [30].…”

Section: Ssr-based Genetic Identification Analysismentioning

confidence: 99%

Characterization of an Early Berry Development Grapevine Somatic Variant (Vitis labrusca L. cv. Isabel Precoce)

Passaia¹,

Margis‐Pinheiro²,

Fialho³

et al. 2014

AJPS

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Over the past 10 years significant advances have been made towards the description of genetics and molecular mechanisms controlling grapevine berry growth. Regardless of this, many aspects of early fruit morphogenesis and its development control remain to be elucidated. In an attempt to understand gene expression patterns associated with the berry growth development, the contrasting phenotype between the cv. Isabel (Vitis labrusca L.) and its early berry development mutant "Isabel Precoce" has been explored by a candidate gene approach. "Isabel Precoce" (Vitis labrusca L.) was confirmed as an EDV (Essentially Derived Variety) of Isabel, with a 30 -35-day reduction in the berry growth phase when compared to the wild type and thus, it constitutes an informative model to investigate many aspects of fruit growth and development. Phenotypic analysis showed that "Isabel Precoce" develops fruits that are smaller in diameter and volume despite of following similar development kinetics. The expression of many genes associated with plant growth and development (MIKC C -type MADS box genes), sugar transport and with the control of flavonoid biosynthetic pathway have been evaluated. The majority of the genes presented a remarkably similar transcription profile. However, a higher induction of transcript accumulation for some genes has been observed in the "Isabel Precoce" genetic background.

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“…1 /4-inch cylindrical ceramic bead (BIO 101-Savant, Carlsbad, CA) 1 and 1.0 ml of CTAB extraction buffer (Lefort and Douglas, 1999) modified with 2X PVP, 2X CTAB, and 2.0% ß-mercaptoethanol. Samples were homogenized in an FP 120 Fastprep machine (BIO 101-Savant) by grinding for 40 s and then cooling them on ice for about one minute.…”

Section: Dna Isolationmentioning

confidence: 99%

Genetic consequences of harvest in a mature second-growth stand of black walnut (Juglans nigra L.)

et al. 2010

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Abstract• This study investigated the short-term changes in neutral genetic variation that would occur if a mature, second-growth, black walnut (Juglans nigra L.) stand was harvested following either a diameter-limited or value-limited cutting regime.• This research tested whether or not the neutral genetic variation within a natural stand of black walnut was disproportionately distributed within the larger or more valuable cohort of trees. To test this hypothesis a complete census of 278 trees from a black walnut stand in Indiana was genotyped using 12 highly polymorphic microsatellites.• Two types of simulated harvests were performed: (1) diameter-limit cuts, where every tree above a given diameter was removed; and (2) selective harvests based on value, where subsets of the most valuable trees were removed. Allelic diversity of the remaining population after each simulated harvest was compared to a corresponding distribution of outcomes from 10 000 random harvests of equal intensity.• None of the simulated harvests resulted in a reduction of allelic diversity significantly greater than that expected under random harvest, indicating that the allelic diversity of the population was evenly distributed across the entire population.• These results indicated that typical harvest scenarios do not per se lead to loss of allelic diversity among trees for this species.

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An efficient micro-method of DNA isolation from mature leaves of four hardwood tree species Acer, Fraxinus, Prunus and Quercus

Cited by 152 publications

References 6 publications

Uso prático de marcadores moleculares para seleção assistida no melhoramento de uvas de mesa apirênicas

Uso prático de marcadores moleculares para seleção assistida no melhoramento de uvas de mesa apirênicas

Characterization of an Early Berry Development Grapevine Somatic Variant (Vitis labrusca L. cv. Isabel Precoce)

Genetic consequences of harvest in a mature second-growth stand of black walnut (Juglans nigra L.)

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