An efficient one-step scheme for the purification of major xanthophyll carotenoids from lettuce, and assessment of their comparative anticancer potential

Saini, Ramesh Kumar; Moon, So Hyun; Enkhtaivan, Gansukh; Keum, Young‐Soo

doi:10.1016/j.foodchem.2018.05.104

Cited by 37 publications

(21 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cytotoxicity of β-cryptoxanthin was assessed by a sulforhodamine B (SRB) assay [10,11]. HeLa and MDCK cells at a concentration of 1.5 × 10 5 cells/mL were separately cultured in a 96-well plate, and incubated under 5% CO 2 for 12 h at 37 • C. The growth medium was discarded, and the cells were washed carefully with 1× PBS (phosphate-buffered saline).…”

Section: Cytotoxic Activities Of Purified β-Cryptoxanthinmentioning

confidence: 99%

“…Our recent results revealed that lutein isolated derived from marigold petals trigger ROS production, inhibit proliferation, and interact with mitochondrial B-cell lymphoma (Bcl)-2 family proteins to activate the caspase-3 mediated apoptosis of human cervical carcinoma HeLa cells [10]. Similarly, several xanthophylls, including 9-Z-neoxanthin, showed potent and selective cytotoxic effects against HeLa cells, with IC 50 values of just 3.8 µM [11].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Chemopreventive Effect of β-Cryptoxanthin on Human Cervical Carcinoma (HeLa) Cells Is Modulated through Oxidative Stress-Induced Apoptosis

et al. 2019

Self Cite

View full text Add to dashboard Cite

The present study was aimed to assess cellular and molecular events involved in the chemopreventive activities of β-cryptoxanthin derived from mandarin oranges (Citrus unshiu Marc.) on human cervical carcinoma (HeLa) cells. In vitro experiments established that β-cryptoxanthin significantly inhibited the proliferation of HeLa cells with the IC50 value of 4.5 and 3.7 µM after 24 and 48 h of treatments, respectively. β-cryptoxanthin-treated HeLa cells exhibited enhanced levels of oxidative stress correlated with significant downregulation of anti-apoptotic Bcl-2, and upregulation of pro-apoptotic Bax mRNA expression. Moreover, β-cryptoxanthin triggered nuclear condensation and disruption of the integrity of the mitochondrial membrane, upregulated caspase-3, -7, and -9 mRNA, and enhanced activation of caspase-3 proteins, resulting in nuclei DNA damage and apoptosis of HeLa cells. Remarkably, TUNEL assay carried out to detect nuclei DNA damage showed 52% TUNEL-positive cells after treatment with a physiological concentration of β-cryptoxanthin (1.0 μM), which validates its potential as an anticancer drug of natural origin.

show abstract

Section: Cytotoxic Activities Of Purified β-Cryptoxanthinmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Chemopreventive Effect of β-Cryptoxanthin on Human Cervical Carcinoma (HeLa) Cells Is Modulated through Oxidative Stress-Induced Apoptosis

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…The role of β-carotene as anti-inflammatory, antioxidant, immunoprotector, dermoprotector, hepatoprotecter, retinoprotective are well known [142]. The food colorant and cosmetic industries use this pigment on a large scale [143]. It has been reported that the beneficial role of this pigment in breast, colon, lung, liver, and skin cancers.…”

Section: β-Carotenementioning

confidence: 99%

Biosynthesis and extraction of high-value carotenoid from algae

Gupta

Seth²,

Maheshwari

et al. 2021

Front. Biosci. (Landmark Ed)

View full text Add to dashboard Cite

Algae possess a considerable potential as bio-refinery for the scale-up production of high-value natural compounds like—carotenoids. Carotenoids are accessory pigments in the light-harvesting apparatus and also act as antioxidants and photo-protectors in green cells. They play important roles for humans, like—precursors of vitamin A, reduce the risk of some cancers, helps in the prevention of age-related diseases, cardiovascular diseases, improve skin health, and stimulates immunity. To date, about 850 types of natural carotenoid compounds have been reported and they have approximated 1.8 billion US$ of global market value. In comparison to land plants, there are few reports on biosynthetic pathways and molecular level regulation of algal carotenogenesis. Recent advances of algal genome sequencing, data created by high-throughput technologies and transcriptome studies, enables a better understanding of the origin and evolution of de novo carotenoid biosynthesis pathways in algae. Here in this review, we focused on, the biochemical and molecular mechanism of carotenoid biosynthesis in algae. Additionally, structural features of different carotenoids are elaborated from a chemistry point of view. Furthermore, current understandings of the techniques designed for pigment extraction from algae are reviewed. In the last section, applications of different carotenoids are elucidated and the growth potential of the global market value of carotenoids are also discussed.

show abstract

“…Not specified [197] Human MDR1 gene-transfected mouse lymphoma; MCF-7 (human breast cancer cell) [198] Neoxanthin (120) Tetraselmis suecica HeLa; A549 cancer cells ↑Cytotoxicity Not specified [199] [218] Various symbols (↑, ↓ and ⊥ ) indicate increase, decrease and inhibition in the obtained variables, respectively.…”

Section: Mrp1mentioning

confidence: 99%

“…Neoxanthin ( 120 ) ( Figure 19 ) is a xanthophyll carotenoid that possesses cytotoxic activity on HeLa and A549 cancer cells. It is even more cytotoxic than violaxanthin [ 199 ].…”

Section: Microalgae Metabolites As Anticancer Drugs With Their Mecmentioning

confidence: 99%

Marine Cyanobacteria and Microalgae Metabolites—A Rich Source of Potential Anticancer Drugs

Mondal¹,

Bose

Banerjee³

et al. 2020

Marine Drugs

View full text Add to dashboard Cite

Cancer is at present one of the utmost deadly diseases worldwide. Past efforts in cancer research have focused on natural medicinal products. Over the past decades, a great deal of initiatives was invested towards isolating and identifying new marine metabolites via pharmaceutical companies, and research institutions in general. Secondary marine metabolites are looked at as a favorable source of potentially new pharmaceutically active compounds, having a vast structural diversity and diverse biological activities; therefore, this is an astonishing source of potentially new anticancer therapy. This review contains an extensive critical discussion on the potential of marine microbial compounds and marine microalgae metabolites as anticancer drugs, highlighting their chemical structure and exploring the underlying mechanisms of action. Current limitation, challenges, and future research pathways were also presented.

show abstract

An efficient one-step scheme for the purification of major xanthophyll carotenoids from lettuce, and assessment of their comparative anticancer potential

Cited by 37 publications

References 23 publications

Chemopreventive Effect of β-Cryptoxanthin on Human Cervical Carcinoma (HeLa) Cells Is Modulated through Oxidative Stress-Induced Apoptosis

Chemopreventive Effect of β-Cryptoxanthin on Human Cervical Carcinoma (HeLa) Cells Is Modulated through Oxidative Stress-Induced Apoptosis

Biosynthesis and extraction of high-value carotenoid from algae

Marine Cyanobacteria and Microalgae Metabolites—A Rich Source of Potential Anticancer Drugs

Contact Info

Product

Resources

About