An efficient protocol for total DNA extraction from the members of order Zingiberales- suitable for diverse PCR based downstream applications

Devi, Khumallambam Devala; Punyarani, Kshetrimayum; Singh, Nandeibam Samarjit; Devi, Huidrom Sunitibala

doi:10.1186/2193-1801-2-669

Cited by 62 publications

(44 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The midrib of the leaf had been removed, as it is the major source of carbohydrate contamination. Several researches have attempted to develop extraction methods by eliminating use of hazardous chemicals, equipment and expensive kits and has developed a CTAB based method for the extraction of high quality total DNA of different species of Zingiberales (Devi et al, 2013). However, that protocol was not successful in the present study for all the species studied therefore, few steps were changed.…”

Section: Discussionmentioning

confidence: 96%

“…Therefore, removal of such contaminants is needed in developing suitable protocols (Devi et al, 2013). The midrib of the leaf had been removed, as it is the major source of carbohydrate contamination.…”

Section: Discussionmentioning

confidence: 99%

“…Genomic DNA was extracted from about 1 g of fresh, young leaves of different Zingiberaceae taxa using Cetyl trimethylammonium Bromide (CTAB) based method (Devi et al, 2013). rDNA ITS ( Table 1) were amplified from genomic DNA by PCR (GoTaq flexi, Promega Inc, USA) in a thermal cycler (Veriti, Applied Biosystems, USA) at 55 0 C annealing temperature.…”

Section: Genetic Analysismentioning

confidence: 99%

See 2 more Smart Citations

Phenetic and genetic characterization of selected economically important species in the family Zingiberaceae

Silva

Herath

Senanayake

et al. 2018

Sri Lankan J. Biol.

View full text Add to dashboard Cite

Zingiberaceae, includes medicinally and economically important monocotyledons mostly distributed in South and South East Asia. A complete taxonomic knowledge of this plant group in Sri Lanka is lacking hence the present study was carried out to characterize and infer phenetic and genetic relationships of eight economically important zingibers. Morphological and anatomical characters of leaves, rhizomes and adventitious roots and phenolic characters of leaves were cluster analysed using MINITAB 17 to derive relationships. A phylogram was obtained from MEGA software using rDNA ITS sequences amplified by PCR from leaf genomic DNA. Costus speciosus was used as the out group in the phylogenetic study. As per the principal component analysis plant height, rhizome diameter, length of rhizome internodes, leaf length, leaf width and length of leaf petiole can be recommended as suitable for characterization of these taxa. rDNA ITS regions may well be used for Zingiber and Curcuma species identification. Combination of morphological, anatomical and phenolic characters can be used in delimiting commonly used Z. officinale cultivars and Curcuma species in Sri Lanka. Further, derived genetic relationships of these taxa would provide better information in characterization of commonly used Zingiber cultivars, Curcuma and Alpinia species in Sri Lanka at the initial developmental stages and would support in quality improvement breeding programs.

show abstract

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Genetic Analysismentioning

confidence: 99%

See 1 more Smart Citation

Phenetic and genetic characterization of selected economically important species in the family Zingiberaceae

Silva

Herath

Senanayake

et al. 2018

Sri Lankan J. Biol.

View full text Add to dashboard Cite

show abstract

“…The method employing the CTAB detergent for cell lysis is widely used for genetically modified organisms (GMO) analysis of raw or processed food; it is also commonly used for plants, especially in combination with other substances such as β-mercaptoethanol (16) (17) (18) . The major difference between the two methods using the CTAB detergent for cell lysis tested in this study was the use of two different enzymes, RNases and Proteinase K, and the 70% ethanol wash (13) , which confirms the high sensitivity of the method with amplification of up to 2.5 cells/mL of acai.…”

Section: Discussionmentioning

confidence: 99%

Extraction of Trypanosoma cruzi DNA from food: a contribution to the elucidation of acute Chagas disease outbreaks

Ferreira

Melandre

Cabral

et al. 2016

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

“…Genomic DNA was isolated from 12 putative transgenic shoots (T 0 ) of A. pallens by CTAB method (Devi et al 2013). The quality of the genomic DNA was checked on 0.8 % agarose gel and quantification done by measuring absorbance (260/280) using a spectrophotometer.…”

Section: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

In vitro regeneration and optimization of factors affecting Agrobacterium mediated transformation in Artemisia Pallens, an important medicinal plant

Alok

Shukla

Pala

et al. 2016

Physiol Mol Biol Plants

View full text Add to dashboard Cite

Artemisia pallens is an important medicinal plant. In-vitro regeneration and multiplication of A. pallens have been established using attached cotyledons. Different growth regulators were considered for regeneration of multiple shoots. An average of 36 shoots per explants were obtained by culturing attached cotyledons on Murashige and Skoog's medium containing 2 mg/L BAP and 0.1 mg/L NAA, after 45 days. The shoots were rooted best on half Murashige and Skoog's medium with respect to media containing 1 mg/L IBA or 1 mg/L NAA. Different parameters such as type of bacterial strains, OD 600 of bacterial culture, co-cultivation duration, concentration of acetosyringone and explants type were optimized for transient expression of the reporter gene. Agrobacterium tumefaciens harbouring pCambia1301 plasmid carrying β-glucuronidase as a reporter gene and hygromycin phosphotransferase as plant selectable marker genes were used for genetic transformation of A. pallens. Hygromycin lethality test showed concentration of 15 mg/L were sufficient to inhibit the growth of attached cotyledons and multiple shoot buds of nontransgenics in selection media.Up to 83 % transient transformation was found when attached cotyledons were co-cultivated with Agrobacterium strain AGL1 for 2 days at 22°C on shoot induction medium. The bacterial growth was eliminated by addition of cefotaxime (200 mg/L) in selection media. T 0 transgenic plants were confirmed by GUS histochemical assay and further by polymerase chain reaction (PCR) using uidA and hpt gene specific primers. The study is useful in establishing technological improvement in A. pallens by genetic engineering.

show abstract

An efficient protocol for total DNA extraction from the members of order Zingiberales- suitable for diverse PCR based downstream applications

Cited by 62 publications

References 23 publications

Phenetic and genetic characterization of selected economically important species in the family Zingiberaceae

Phenetic and genetic characterization of selected economically important species in the family Zingiberaceae

Extraction of Trypanosoma cruzi DNA from food: a contribution to the elucidation of acute Chagas disease outbreaks

In vitro regeneration and optimization of factors affecting Agrobacterium mediated transformation in Artemisia Pallens, an important medicinal plant

Contact Info

Product

Resources

About