An Efficient Reproductive Method for Irs2-/- Mice with C57BL/6JJcl Genetic Background

Hashimoto, Haruo; Eto, Tomoo; Kamisako, Tsutomu; Hoya, Naoko; Hatakeyama, Teruhiko; Arai, Toshiro; Yokosuka, Makoto; Ohnishi, Yasuyuki; Ito, Mamoru; Hioki, Kyoji; Suzuki, Ryo; Ohsugi, Mitsuru; Saito, Muneo; Ueyama, Yoshito; Yamauchi, Toshimasa; Kubota, Naoto; Tobe, Kazuyuki; Kadowaki, Takashi; Tamaoki, Norikazu; Nomura, Tatsuji; Kosáka, Kinori

doi:10.1538/expanim.57.407

Cited by 4 publications

(5 citation statements)

References 13 publications

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“…Data for the B6J-Irs2 -/-mouse are presented in this paper. The descriptions and data in the following sections are cited and abstracted from previous publications [2,[7][8][9][10].…”

Section: Irs2mentioning

confidence: 99%

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Study on Establishment of Congenic Strains and Screening of Characteristics in IRS-2 Deficient Mice to Support Translational Research on Type 2 Diabetes

Hashimoto

2011

Exp. Anim.

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Abstract:In research into type 2 diabetes, diet-based approaches, i.e., nutritional intake, are important approaches for therapeutic research. We would like to make the following two proposals from the standpoint of laboratory animal science for reproducible animal studies using type 2 diabetes mouse models. These include congenic strains of diabetes mouse models and improvement of diets used in daily care and management. In this research, the Irs2homo-knockout mouse with both impaired glucose tolerance and insulin resistance, and thus type 2 diabetes, was established as a congenic strain. The effect of the genetic background on the onset of diabetes was examined. Next, we discussed which diets are appropriate for general care and management of mouse models in which the pathophysiology is controlled by nutritional conditions. Therefore, we prepared diets by converting the current Japanese and US diets to mice and adjusting the diet contents accordingly. We compared the insulin signals such as those of the liver, pancreas and white fat. We were thus able to establish an evaluation system closer to diabetes in the current population. Using this data as an example, we should consider the quality and ordinary diet of animals as important factors in animal experiments.

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“…Data for the B6J-Irs2 -/-mouse are presented in this paper. The descriptions and data in the following sections are cited and abstracted from previous publications [2,[7][8][9][10].…”

Section: Irs2mentioning

confidence: 99%

“…Next, the reproductive efficiency of B6J-Irs2 -/-mice produced by both natural mating and reproductive engineering was investigated [10]. and that for the female Irs2 +/-mouse × male Irs2 -/-mouse was 0.7-1.1.…”

Section: Investigation Of the Reproductive Efficiency Of The B6j-irs2mentioning

confidence: 99%

Study on Establishment of Congenic Strains and Screening of Characteristics in IRS-2 Deficient Mice to Support Translational Research on Type 2 Diabetes

Hashimoto

2011

Exp. Anim.

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“…Spermatozoa with magnetic beads only were used as control (control group). ICSI, IVF and ET were performed in C57BL/6JJcl mice (CLEA Japan, Inc., Tokyo, Japan) at 10-12 weeks of age as followed the past reports [4,17].PCR bands were detected in mouse spermatozoa, testes, mouse genomic DNA, and SRY cDNA cloned into T-vectors by specific cDNA primers (Fig. 3, Lanes 2-6) and in mouse genomic DNA and SRY genomic regions cloned into Tvectors only by genomic DNA primers (Fig.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

Application of a New Convenience Gender Sorting Method for Mouse Spermatozoa to Mouse Reproductive Engineering Technology

Hashimoto

Eto

Suemizu

et al. 2013

The Journal of Veterinary Medical Science

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ABSTRACT. In this study, we attempted to apply new convenience gender sorting methods using sex-determining region Y (SRY) gene expression on Y spermatozoa to mice. Mouse spermatozoa labeled with Cy3-SRY antibody conjugate were used for intracytoplasmic sperm injection (ICSI). In addition, spermatozoa conjugated with SRY antibody were conjugated with magnetic beads (Mag) and were pulled to the bottom of the medium. The supernatant of the medium was used for in vitro fertilization (IVF). The rate of males reproduced by ICSI using the spermatozoa conjugated with Cy3-SRY antibody was 86.1%. The female proportion reproduced by IVF using the spermatozoa separated in the supernatant after Mag-SRY antibody conjugation was 67.3%. These gender sorting methods are effective for the reproduction of transgenic mice. KEY WORDS: gender sorting, in vitro fertilization, Y spermatozoa.doi: 10.1292/jvms.12-0303; J. Vet. Med. Sci. 75(2): 231-235, 2013 The goal of this research was to establish a new convenience gender sorting method for mammals, which has been difficult in the past. As a first step, a mouse reproductive engineering technology was established. The establishment of gender sorting technology is essential for the reproduction of laboratory animals [7], livestock [1,3,6] and humans [2,5,8,15]. The reproduction of mice and rats by reproductive engineering technology included intracytoplasmic sperm injection (ICSI), in vitro fertilization (IVF) and embryo transfer (ET), which are important technologies in laboratory animal facilities. Therefore, we attempted gender sorting targeted at mice with an established reproductive technology. There is high demand for females for the production of mice. For example, the reproduction of mice carrying several mutant genes, such as IRS-1 deficient Gck −/+ mice [12] and PPARγdeficient ob/ob mice [9], using reproductive engineering technology requires many females in the production process, although a male is required in the final stage. When no accidental bias occurs, the gender ratio after ET and Cesarean section is usually 50:50 (female:male). Modi et al. (2005) reported that SRY transcription occurs in spermatozoa in humans [10]. From their study, we assumed that the same phenomenon occurs in mouse spermatozoa. If this was true, then the sex-determining region Y (SRY) protein may also be produced by spermatozoa. Therefore, we examined the previous report indicating that SRY transcription occurs in spermatozoa in humans [10] and contrived a new technology for gender sorting using fluorescence or magnetic beads via SRY antibody, using mice as an example of mammals.Because the SRY gene consists only of an exon, SRY gene expression was confirmed by two polymerase chain reaction (PCR) experiments using primers designed with a specific sequence of intra-exons to detect cDNA or a sequence ranging from the 5'-genomic region to the exon to detect genomic DNA (Fig. 1). To generate positive control vectors, RNA and genomic DNA were extracted from the sperm and testes of C57BL/6JJcl mice at...

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“…However, the parturition rates or pup survival rates in production using reproductive engineering are markedly reduced by the pathophysiology of transgenic or knockout mice [3]. There are many technical factors involved in production using reproductive engineering, and the perinatal factors are administration of progesterone (P4), Caesarean section and handling of foster pups.…”

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Comparative Study of Doses of Exogenous Progesterone Administration Needed to Delay Parturition in Jcl:MCH(ICR) Mice

et al. 2010

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Abstract:The effects of progesterone (P4) used in physiological studies and in delayed parturition in reproductive engineering were examined. A dose of 0.25, 0.5, 1, or 2 mg of P4 was repeatedly administered to Jcl:MCH(ICR) mice on days 17 and 18 of gestation, and plasma concentrations of P4 were investigated. The P4 concentrations in mothers and fetuses after administration of exogenous P4 were no differences between doses of 1 and 2 mg. Jcl:MCH(ICR) mothers administered a P4 dose of 1 mg did not give birth. Therefore, we consider 2 mg of P4 is an overdose and that it is evident that a dose of 1 mg P4 is sufficient to induce delayed parturition.

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An Efficient Reproductive Method for Irs2-/- Mice with C57BL/6JJcl Genetic Background

Cited by 4 publications

References 13 publications

Study on Establishment of Congenic Strains and Screening of Characteristics in IRS-2 Deficient Mice to Support Translational Research on Type 2 Diabetes

Study on Establishment of Congenic Strains and Screening of Characteristics in IRS-2 Deficient Mice to Support Translational Research on Type 2 Diabetes

Application of a New Convenience Gender Sorting Method for Mouse Spermatozoa to Mouse Reproductive Engineering Technology

Comparative Study of Doses of Exogenous Progesterone Administration Needed to Delay Parturition in Jcl:MCH(ICR) Mice

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