An efficient strategy for large‐scale preparation of low polarity gingerols directly from ginger crude extract by high‐speed countercurrent chromatography with different rotation mode

Abstract

This study presents an efficient strategy for large‐scale preparation of low polarity gingerols directly from ginger crude extract by high‐speed countercurrent chromatography with different rotation mode. The ultrasonic‐assisted extraction conditions were optimized by response surface methodology and the results showed the major low polarity gingerols could be well enriched under the optimized extraction conditions. Then the crude extract without any pretreatment was directly separated by high‐speed countercurrent chromatography with different rotation mode using n‐hexane/ethyl acetate/methanol/water (6:4:6:4, v/v/v/v) as the solvent system. In about 400 min, five major gingerols including 150 mg of [6]‐gingerol, 50 mg of [8]‐gingerol, 20 mg of [6]‐shogaol, 43 mg of [6]‐dehydrogingerdione, and 40 mg of [10]‐gingerol were obtained from 1.2 g of crude extract in a single run with repeated injection. Their structures were identified by 1H‐NMR spectroscopy.

Target‐guided isolation and purification of cyclooxygenase‐2 inhibitors from Meconopsis integrifolia (Maxim.) Franch. by high‐speed counter‐current chromatography combined with ultrafiltration liquid chromatography

“…Within this framework, “ Y ” signifies the projected outcome, while β 0 is the model's intercept. The coefficients β 1 , β 2 , and β 3 pertain to the linear elements, β 11 , β 22 , and β 33 are linked with quadratic elements, and β 12 , β 13 , and β 23 are indicative of the interactive elements concerning the independent variables denoted as X 1 , X 2 , and X 3 in the evaluation [19].…”

“…Response surface methodology (RSM) is acknowledged as a powerful method for fine-tuning the extraction procedures of active substances [18]. Leveraging precise mathematical and statistical techniques allows for the streamlined delineation of optimal conditions for extraction [19]. Moreover, it is extensively utilized to pinpoint the ideal conditions for operations [20][21][22][23].…”

Target-Guided Isolation and Purification of Antioxidants from Urtica laetevirens Maxim. by HSCCC Combined with Online DPPH-HPLC Analysis

“…Within this framework, "Y" signifies the projected outcome, while β 0 is the model's intercept. The coefficients β 1 , β 2 , β 3 pertain to the linear elements, β 11 , β 22 , β 33 are linked with quadratic elements, and β 12 , β 13 , β 23 are indicative of the interactive elements concerning the independent variables denoted as X 1 , X 2 , and X 3 in the evaluation [12].…”

“…Response surface methodology (RSM) is acknowledged as a powerful instrument in fine-tuning the extraction procedures for active substances [11]. Leveraging precise mathematical and statistical techniques, it allows for the streamlined delineation of optimal conditions for extraction [12]. Moreover, it is extensively utilized to pinpoint the ideal conditions for operation [13][14][15][16].…”