An Efficient System for Regenerating Taraxacum Pieninicum Pawł. from Seedling Explants

Abstract

An efficient micropropagation system for Taraxacum pieninicum using seedling explants germinated in vitro is described. Shoot tips and fragments of cotyledons, hypocotyls and roots were isolated from several-day-old seedlings. The highest response, 100% frequency with 12.3 axillary shoots/explant, was from shoot tips on medium supplemented with 0.5 mg L -1 BA and 0.05 mg L -1 NAA. In subsequent subcultures the number of shoots was significantly higher on all explants cultured on medium containing 0.25 and 0.5 mg L -1 BA, and the multiplication rate was highest (20 shoots/explant) in the 4th passage. Shoots rooted on MS and 1/2 MS medium; the highest rooting frequency was 90% and the highest number of roots 2.7/shoot. Rooted plants showed 96.2% survival in sterile soil:sand, and 100% survival in hydroponic culture. Regenerated plants flowered in the second year after acclimatization and yielded viable seeds. This protocol for obtaining complete plants through micropropagation may prove useful for conservation of the genetic resources of this and other endangered species.K Ke ey y w wo or rd ds s: : Taraxacum pieninicum, endangered species, in vitro culture, multiple shoots, benzylaminopurine, hydroponic culture, fertile plants, conservation.

Efficient long-term conservation of Taraxacum pieninicum synthetic seeds in slow growth conditions

“…The pH of the medium was adjusted to 5.8 before autoclaving. Cultures were incubated in continuous white fluorescent light (80 μmol·m −2 ·s −1 ) at 26 ± 1 °C (optimal growth conditions described by Trejgell et al 2013). …”

“…In the previous studies the protocol for efficient in vitro multiplication and procedure for shoot tips storage of this species at 10 °C were developed (Trejgell et al 2013;Kamińska et al 2016) However, despite the low storage temperature, shoot growth has not been sufficiently inhibited, which has negatively affected the quality of the stored material. The aim of the current study was to inhibit T. pieninicum shoots growth by lowering the temperature to 4 °C and to limit damage of meristem through alginate-encapsulation of the shoot tips.…”

Micropropagation of Inula germanica L. from the Seedlings Explants

“…Shoots have been rooted without auxin in many Asteraceae species. High rooting response was achieved for species such as Echinacea purpurea (Korach et al, 2002), Cirsium pannonicum (Trejgell et al, 2012), Arnica montana (Surmacz-Magdziak and Sugier, 2012), Artemisia 3.4 ± 0.7 a B 67.9 ± 5.0 4.9 ± 0.9 a B Hypocotyl 83.3 ± 6.7 6.2 ± 1.1 b A 84.6 ± 11.4 2.2 ± 0.3 b B 96.0 ± 3.2 3.7 ± 0.5 a C 100.0 ± 0.0 3.0 ± 0.5 a BC Cotyledon 50.0 ± 10.0 10.1 ± 0.8 a A 100.0 ± 0.0 6.6 ± 0.9 a B 98.9 ± 0.2 4.2 ± 0.4 a C 100.0 ± 0.0 4.2 ± 0.8 a C *Means ± standard error, means with small different letter within columns and capital letters within the rows indicate significant differences by ANOVA followed by Kruskal-Wallis test at p<0.05 (Rasool et al, 2013), and Taraxacum pieninicum (Trejgell et al, 2013). Addition of IBA to medium significantly increased the percentage of rooted shoots of I. germanica ( Fig.…”

“…Moreover, efficient and rapid clonal regeneration method has been shown to be a great tool in the protection of rare and threatened species of the Asteraceae family e.g. Centaurea ultreiae (Mallón et al, 2011), Carlina onopordipholia (Trejgell et al, 2011), Arnica montana (Surmacz-Magdziak and Sugier, 2012), or Taraxacum pieninicum (Trejgell et al, 2013) and others (Amin et al, 2013).…”

Chromosome Numbers in 10 Taraxacum Species From Poland

“…It grows well in cultivation, a fortunate feature should it become extinct in nature. Also, recently an efficient system of in vitro micropropagation has been developed for it (Trejgell et al, 2013).…”