An efficient system for the production of clonal plantlets of the medicinally important aromatic plant: Salvia africana-lutea L.

Makunga, Nokwanda P.; Staden, J. Van

doi:10.1007/s11240-007-9305-5

Cited by 55 publications

(42 citation statements)

References 26 publications

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“…Interestingly, the regenerated plantlets flowered in vitro after 3 months of subculture (Figure 1(H)). In vitro flowering was also reported previously in other plants [20,21]. In report of Makunga and Staden [20], in vitro flowering in Salvia Africana-lutea became apparent seasonally throughout the year.…”

Section: −1supporting

confidence: 78%

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An Efficient Method for Plant Regeneration from Calli of Swertia mussotii, an Endangered Medicinal Herb

He¹,

Xu²,

Yang³

et al. 2012

AJPS

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Swertia mussotii Franch is an endangered medicinal plant in the Qinghai-Tibet Plateau. Its regeneration from callus culture is very difficult. In this study, an efficient method for plant regeneration was developed from its calli. The calli derived from young stem explants of S. mussotii were cultured at two types of temperature treatments to test their efficiency of shoot regeneration. When the calli were cultured at variable temperature (VT, 20˚C during the day and 10˚C at night) treatment, the adventitious shoots were formed at each combination tested. However, that did not occur when the calli were cultured at constant temperature (CT, 25˚C) treatment. The best response (53.7%) of plantlet regeneration was obtained on Murashige and Skoog (MS) medium containing 3 mg·l −1 6-benzylaminopurine with 0.5 mg·l −1 α-naphthaleneacetic acid in the VT treatment. The regenerated plantlets were rooted on half-strength MS medium without growth regulators. They flowered in the following subculture. The results indicate that the treatment of day-night temperature difference is a critical factor to callus differentiation in S. mussotii. This protocol can be used for conservation as well as mass propagation of this medicinal plant.

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Section: −1supporting

confidence: 78%

“…In vitro flowering was also reported previously in other plants [20,21]. In report of Makunga and Staden [20], in vitro flowering in Salvia Africana-lutea became apparent seasonally throughout the year. In Psygmorchis pusilla, a positive relationship was observed between long days and floral spike formation [21].…”

Section: −1supporting

confidence: 78%

An Efficient Method for Plant Regeneration from Calli of Swertia mussotii, an Endangered Medicinal Herb

He¹,

Xu²,

Yang³

et al. 2012

AJPS

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“…The comparative effect of different PGRs in stimulating shoot multiplication response showed more effectiveness of BAP with NAA than BAP with IAA and it is the fact that total hormonal response has been gathered from initial culture to subsequent cultures. The ratio of auxin and cytokinin 1: 2-1:3 was more effective in organogenic response and adventitious shoot induction was reported in several studies (Makunga and Staden, 2008;Sen et al, 2009). The highest response of callus initiation of 60% was found in media supplemented with KI (6.97, 13.9 µM) and IAA (2.85, 5.71 µM) after 4 weeks of culture.…”

Section: Discussionsupporting

confidence: 54%

“…According to Coenen and Lomax (1997), auxins are known to exhibit synergistic, antagonistic and additive interactions with cytokinins at multiple levels depending on the plant species and tissue types in regulating responses. Similarly, Mohebalipour et al (2012) observed that addition of BAP and IAA to MS medium is more effective than other hormones which significantly increased the number of shoot induction per explants in most of the genotypes of M. officinalis L. The plant regeneration capacity of 2000 to 3000 plants from one shoot after long subculture of Salvia africana-lutea L. was reported (Makunga and Staden, 2008) where BA and IAA were used for subcultures. Plant regeneration ability in the culture medium is usually enhanced by the addition of auxins and cytokinins which is very common (Varshney et al, 1996;Fatima et al, 2009).…”

Section: Discussionmentioning

confidence: 91%

Effect of plant growth regulators on regeneration of the endangered medicinal plant Calligonum comosum L. Henry in the Kingdom of Bahrain

Sadeq¹,

Pathak²,

Salih³

et al. 2014

Afr. J. Biotechnol.

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The use of in vitro techniques for conserving plant biodiversity and protecting rare and endangered multipurpose plant species is considered as one of the most important ex-situ conservation policies. Development of an efficient in vitro regeneration protocol of Calligonum comosum is important and that has achieved to protect the endangered multipurpose medicinally important desert plant in the Kingdom of Bahrain. Nodal segments were used as explants source and the effect of various plant growth regulators (PGRs) were studied for responses and to regenerate the whole plants in modified Murashige and Skoog (MS) media through direct and indirect organogenesis via callus induction. 50% explants of C. comosum responded to initiate shoot in presence of 4.44 µM BAP with 2.68 µM NAA after four weeks of culture while 40% shoot initiation response was the highest value in presence of 9.29 µM KI with 5.37 µM NAA after 4 weeks of culture among the treatments of KI with NAA. The highest callus induction rate of 100% was found in media containing 9.29 µM KI and 5.37 µM NAA after four weeks. Multiple initial shoots those originated from nodal segments develop calli and showed organogenic differentiation of shoots in presence of BAP and IAA. The highest shoot multiplication frequency of 15 was observed while the shoots initiated in media contained 4.44 µM BAP with 2.85 µM IAA and were transferred to 8.56 µM IAA with 2.22 µM BAP. Shoot multiplication and shoot regeneration capacity was compared in different media and the highest performance of 234 shoots /explants after second multiplication was observed while shoots initiated in presence of 13.3 µM BAP and 5.71 µM IAA. As a precautionary approach to conserve the endangered medicinal plant species in the Kingdom of Bahrain, the application of in vitro culture is considered as an important alternative method in ex situ conservation strategy in the present study.

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“…Activated charcoal or carbon (AC) had been reported to be a useful supplement in the plant tissue culture medium for several purposes such as improving rooting [35], shoot elongation [36], preventing necrosis [37], and preventing browning [38]. The fine network of pores and large surface area of AC serves as a useful scavenger to adsorb inhibitory phenolic compounds.…”

Section: Direct Organogenesismentioning

confidence: 99%

Sterilization of Hibiscus rosa-sinensis L. Vegetative Explants Sourced from Plants Grown in Open Environment and Influences of Organic Ingredients on In Vitro Direct Regeneration

Tiong¹,

Ong

Namasivayam³

et al. 2012

AJPS

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This paper reports on the effects of organic ingredients in facilitating direct shoot regeneration from nodal explants of Hibiscus rosa-sinensis L. This paper also compares the sterilization conditions for 3 types of explants (node, internode, and shoot tip) harvested from an open field. The optimized sterilization conditions for the explants were 40% Clorox-20 min exposure, 10% Clorox-15 min exposure, and 5% Clorox-40 min exposure for the node, internode and shoot tip, respectively. In the direct shoot regeneration using the nodal explants, we found MS medium containing 40 g/L sucrose, 0.3% (w/v) activated charcoal, and supplementations with myo-inositol, thiamine and nicotinic acid were suitable. The in vitro shoot survival rate was 30% with a mean leaf numbers of 2.68 produced, and a mean leaf length of 1.71 cm achieved after 5 weeks of culture on the modified medium.

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An efficient system for the production of clonal plantlets of the medicinally important aromatic plant: Salvia africana-lutea L.

Cited by 55 publications

References 26 publications

An Efficient Method for Plant Regeneration from Calli of <i>Swertia mussotii</i>, an Endangered Medicinal Herb

An Efficient Method for Plant Regeneration from Calli of <i>Swertia mussotii</i>, an Endangered Medicinal Herb

Effect of plant growth regulators on regeneration of the endangered medicinal plant Calligonum comosum L. Henry in the Kingdom of Bahrain

Sterilization of <i>Hibiscus rosa-sinensis</i> L. Vegetative Explants Sourced from Plants Grown in Open Environment and Influences of Organic Ingredients on <i>In Vitro</i> Direct Regeneration

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An efficient system for the production of clonal plantlets of the medicinally important aromatic plant: Salvia africana-lutea L.

Cited by 55 publications

References 26 publications

An Efficient Method for Plant Regeneration from Calli of &lt;i&gt;Swertia mussotii&lt;/i&gt;, an Endangered Medicinal Herb

An Efficient Method for Plant Regeneration from Calli of &lt;i&gt;Swertia mussotii&lt;/i&gt;, an Endangered Medicinal Herb

Effect of plant growth regulators on regeneration of the endangered medicinal plant Calligonum comosum L. Henry in the Kingdom of Bahrain

Sterilization of &lt;i&gt;Hibiscus rosa-sinensis&lt;/i&gt; L. Vegetative Explants Sourced from Plants Grown in Open Environment and Influences of Organic Ingredients on &lt;i&gt;In Vitro&lt;/i&gt; Direct Regeneration

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An Efficient Method for Plant Regeneration from Calli of <i>Swertia mussotii</i>, an Endangered Medicinal Herb

An Efficient Method for Plant Regeneration from Calli of <i>Swertia mussotii</i>, an Endangered Medicinal Herb

Sterilization of <i>Hibiscus rosa-sinensis</i> L. Vegetative Explants Sourced from Plants Grown in Open Environment and Influences of Organic Ingredients on <i>In Vitro</i> Direct Regeneration