An electroactive hybrid biointerface for enhancing neuronal differentiation and axonal outgrowth on bio-subretinal chip

Yang, Jiawei; Chen, Chong-You; Yu, Zih-Yu; Chung, Johnson; Liu, Xiao; Wu, Chung-Yu; Chen, Guan-Yu

doi:10.1016/j.mtbio.2022.100253

Cited by 8 publications

(4 citation statements)

References 67 publications

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“…In addition, Yang et al developed functional annealed graphene oxide-collagen (aGO-Col) that included electroactive 3D crumpled structures [56]. They showed that aGO-Col composites enhanced the differentiation efficiency of PC12 cells in the presence of NGF.…”

Section: Electrical Stimulationmentioning

confidence: 99%

Physical Stimulation Methods Developed for In Vitro Neuronal Differentiation Studies of PC12 Cells: A Comprehensive Review

Tominami,

Kudo,

Noguchi

et al. 2024

IJMS

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PC12 cells, which are derived from rat adrenal pheochromocytoma cells, are widely used for the study of neuronal differentiation. NGF induces neuronal differentiation in PC12 cells by activating intracellular pathways via the TrkA receptor, which results in elongated neurites and neuron-like characteristics. Moreover, the differentiation requires both the ERK1/2 and p38 MAPK pathways. In addition to NGF, BMPs can also induce neuronal differentiation in PC12 cells. BMPs are part of the TGF-β cytokine superfamily and activate signaling pathways such as p38 MAPK and Smad. However, the brief lifespan of NGF and BMPs may limit their effectiveness in living organisms. Although PC12 cells are used to study the effects of various physical stimuli on neuronal differentiation, the development of new methods and an understanding of the molecular mechanisms are ongoing. In this comprehensive review, we discuss the induction of neuronal differentiation in PC12 cells without relying on NGF, which is already established for electrical, electromagnetic, and thermal stimulation but poses a challenge for mechanical, ultrasound, and light stimulation. Furthermore, the mechanisms underlying neuronal differentiation induced by physical stimuli remain largely unknown. Elucidating these mechanisms holds promise for developing new methods for neural regeneration and advancing neuroregenerative medical technologies using neural stem cells.

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Section: Electrical Stimulationmentioning

confidence: 99%

Physical Stimulation Methods Developed for In Vitro Neuronal Differentiation Studies of PC12 Cells: A Comprehensive Review

Tominami,

Kudo,

Noguchi

et al. 2024

IJMS

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“…There are two categories of electrode materials proposed for retinal prostheses: inorganics-such as metals, silicones, and carbon-based materials, and organics-such as polyimide and polydimethylsiloxane (PDMS) [119]. The literature consistently demonstrated that smaller-sized electrodes optimize electrical signaling due to comparable dimensionality to their neural targets; however, the innate physical limitations with current electrodes require further inquiry [120].…”

Section: Materials Sciencementioning

confidence: 99%

Retinal Prostheses: Engineering and Clinical Perspectives for Vision Restoration

Mina

Sahyoun

et al. 2023

Sensors

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A retinal prosthesis, also known as a bionic eye, is a device that can be implanted to partially restore vision in patients with retinal diseases that have resulted in the loss of photoreceptors (e.g., age-related macular degeneration and retinitis pigmentosa). Recently, there have been major breakthroughs in retinal prosthesis technology, with the creation of numerous types of implants, including epiretinal, subretinal, and suprachoroidal sensors. These devices can stimulate the remaining cells in the retina with electric signals to create a visual sensation. A literature review of the pre-clinical and clinical studies published between 2017 and 2023 is conducted. This narrative review delves into the retinal anatomy, physiology, pathology, and principles underlying electronic retinal prostheses. Engineering aspects are explored, including electrode–retina alignment, electrode size and material, charge density, resolution limits, spatial selectivity, and bidirectional closed-loop systems. This article also discusses clinical aspects, focusing on safety, adverse events, visual function, outcomes, and the importance of rehabilitation programs. Moreover, there is ongoing debate over whether implantable retinal devices still offer a promising approach for the treatment of retinal diseases, considering the recent emergence of cell-based and gene-based therapies as well as optogenetics. This review compares retinal prostheses with these alternative therapies, providing a balanced perspective on their advantages and limitations. The recent advancements in retinal prosthesis technology are also outlined, emphasizing progress in engineering and the outlook of retinal prostheses. While acknowledging the challenges and complexities of the technology, this article highlights the significant potential of retinal prostheses for vision restoration in individuals with retinal diseases and calls for continued research and development to refine and enhance their performance, ultimately improving patient outcomes and quality of life.

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“…Several reports have indicated impaired metabolism and dysregulated gene expression in patient-specific iPSC-derived RPEs from AMD patients, as well as the feasibility of using iPSC-derived RPEs to investigate AMD pathogenic mechanisms [43][44][45][46][47][48] (Figure 5). In addition to primary human adult RPEs and iPSCderived RPEs, some advanced in vitro cell models, such as human stem cell-derived 3D organoid systems [49][50][51][52], subretinal chips [51][52][53], and bioprinting platforms [49], have also been used to improve the efficiency and therapeutic translatability of preclinical studies and drug screening. The multi-modal chip also sets up a novel platform of bio-subretinal prostheses that are implantable and may have the potential to restore vision [53].…”

Section: Application Of Ipsc-derived Rpes In Disease Modeling and Cel...mentioning

confidence: 99%

“…In addition to primary human adult RPEs and iPSCderived RPEs, some advanced in vitro cell models, such as human stem cell-derived 3D organoid systems [49][50][51][52], subretinal chips [51][52][53], and bioprinting platforms [49], have also been used to improve the efficiency and therapeutic translatability of preclinical studies and drug screening. The multi-modal chip also sets up a novel platform of bio-subretinal prostheses that are implantable and may have the potential to restore vision [53]. The development of iPSC technologies made in vitro studies of pluripotent differentiation and tissue transplantations possible.…”

Section: Application Of Ipsc-derived Rpes In Disease Modeling and Cel...mentioning

confidence: 99%

Pluripotent Stem Cells in Clinical Cell Transplantation: Focusing on Induced Pluripotent Stem Cell-Derived RPE Cell Therapy in Age-Related Macular Degeneration

Yang

Hsiao

Chang

et al. 2022

IJMS

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Human pluripotent stem cells (PSCs), including both embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), represent valuable cell sources to replace diseased or injured tissues in regenerative medicine. iPSCs exhibit the potential for indefinite self-renewal and differentiation into various cell types and can be reprogrammed from somatic tissue that can be easily obtained, paving the way for cell therapy, regenerative medicine, and personalized medicine. Cell therapies using various iPSC-derived cell types are now evolving rapidly for the treatment of clinical diseases, including Parkinson’s disease, hematological diseases, cardiomyopathy, osteoarthritis, and retinal diseases. Since the first interventional clinical trial with autologous iPSC-derived retinal pigment epithelial cells (RPEs) for the treatment of age-related macular degeneration (AMD) was accomplished in Japan, several preclinical trials using iPSC suspensions or monolayers have been launched, or are ongoing or completed. The evolution and generation of human leukocyte antigen (HLA)-universal iPSCs may facilitate the clinical application of iPSC-based therapies. Thus, iPSCs hold great promise in the treatment of multiple retinal diseases. The efficacy and adverse effects of iPSC-based retinal therapies should be carefully assessed in ongoing and further clinical trials.

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An electroactive hybrid biointerface for enhancing neuronal differentiation and axonal outgrowth on bio-subretinal chip

Cited by 8 publications

References 67 publications

Physical Stimulation Methods Developed for In Vitro Neuronal Differentiation Studies of PC12 Cells: A Comprehensive Review

Physical Stimulation Methods Developed for In Vitro Neuronal Differentiation Studies of PC12 Cells: A Comprehensive Review

Retinal Prostheses: Engineering and Clinical Perspectives for Vision Restoration

Pluripotent Stem Cells in Clinical Cell Transplantation: Focusing on Induced Pluripotent Stem Cell-Derived RPE Cell Therapy in Age-Related Macular Degeneration

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